Skip to main content
SpringerLink
Log in
Book cover

The Algae World pp 555–579Cite as

Culturing Algae

  • Chapter

  • 5673 Accesses

Part of the book series: Cellular Origin, Life in Extreme Habitats and Astrobiology ((COLE,volume 26))

Abstract

Culturing algae is very important not only for their experimental studies but also for industrial production. Therefore, unialgal culture is very important which are free from contamination. In order to obtain unialgal cultures of selected algal species various culture techniques and maintenance is required. Sterilization of all the glassware and disinfectant treatment of the microalgae should be taken care before the start of culture. Common culture media for both micro and macroalgae are prepared and store at 4 °C so that there should be less contamination. The culture can be maintained both in solid and liquid media. But for the preservation of cultures for long time it is preferred to maintain in liquid cultureὑ.

This is a preview of subscription content, log in via an institution.

Chapter
USD   29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD   229.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD   299.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book
USD   299.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Learn about institutional subscriptions

References

  • Aiba S, Ogawa T (1977) Assessment of growth yield of a blue green alga: Spirulina platensis in axenic and continuous culture. J Gen Microbiol 102:179–182

    Article  Google Scholar 

  • Allen MM (1968) Simple conditions for growth of unicellular blue-green algae on plates. J Phycol 4:1–4

    Article  CAS  Google Scholar 

  • Allen MM, Stanier RY (1968) Growth and division of some unicellular blue green algae. J Gen Microbiol 51:270–277

    Google Scholar 

  • Andersen RA (ed) (2005) Algal culturing techniques. Academic, Burlington, 578pp

    Google Scholar 

  • Andersen RA, Kawachi M (2005) Traditional microalgae isolation techniques. In: Andersen RA (ed) Algal culturing techniques. Elsevier Academic Press, San Diego, pp 83–101

    Google Scholar 

  • Beijerinck MW (1890) Culturversuche mit Zoochlorellen, Lichengonidien und anderen niederen Algen. Bot Ztg 48:725–739

    Google Scholar 

  • Bischoff HW, Bold HC (1963) Phycological studies IV. Some soil algae from enchanted rock and related algal species, vol 6318. University of Texas, Austin, pp 1–95

    Google Scholar 

  • Bold HC (1942) The cultivation of algae. Bot Rev 8:69–138

    Article  Google Scholar 

  • Bold HC (1949) The morphology of Chlamydomonas chlamydobama sp. nov. Bull Torrey Bot Club 76:101–108

    Article  Google Scholar 

  • Carlozzi P, Pushparaj B, DeglInnocenti A, Capperucci A (2006) Growth characteristics of Rhodopseudomonas palustris cultured outdoors, in an underwater tubular photobioreactor, and investigation of photosynthetic efficiency. Appl Microbiol Biotechnol 73:789–795

    Article  CAS  PubMed  Google Scholar 

  • Carvalho AP, Meireles LA, Malcata FX (2006) Microalgal reactors: a review of enclosed system designs and performances. Biotechnol Prog 22:1490–1506

    Article  CAS  PubMed  Google Scholar 

  • Chen W, Yue L (2005) Isolation and determination of cultural characteristics of a new highly CO2 tolerant fresh water microalgae. Ener Cons Manag 46:1868–1876

    Article  Google Scholar 

  • Chu SP (1942) The influence of the mineral composition of the medium on the growth of planktonic algae. Part 1. Methods and culture media. J Ecol 30:284–325

    Article  CAS  Google Scholar 

  • Clark WJ, Sigler WF (1963) Method of concentrating phytoplankton samples using membrane filters. Limnol Oceanogr 8:127–129

    Article  Google Scholar 

  • Cohn SA, Pickett-Heaps JD (1988) The effects of colchicines and dinitrophenol on the in vivo rates of anaphase A and B in the diatom Surirella. Eur J Cell Biol 46:523–530

    CAS  PubMed  Google Scholar 

  • Cohn SA, Farrell JF, Munro JD, Ragland RL, Weitzell RE Jr, Wibisono BL (2003) The effect of temperature and mixed species composition on diatom motility and adhesion. Diatomol Res 18:225–243

    Article  Google Scholar 

  • Converti A, Lodi A, Del Borghi A, Solisio C (2006) Cultivation of Spirulina platensis in a combined airlift-tubular system. Biochem Eng J 32:13–18

    Article  CAS  Google Scholar 

  • Devi SS (2008) Screening, isolation and laboratory scale culture of microalgae for biodiesel production. M.Phil dissertation, Delhi University, Delhi

    Google Scholar 

  • Guillard RRL (1975) Culture of phytoplankton for feeding marine invertebrates. In: Smith WL, Chanley MH (eds) Culture of marine invertebrate animals. Plenum Press, New York, pp 26–60

    Google Scholar 

  • Guillard RRL, Morton SL (2003) Culture methods. In: Hallegraeff GM, Anderson DM, Cembella AD (eds) Manual on harmful marine microalgae. UNESCO, Paris, pp 77–97

    Google Scholar 

  • Guillard RRL, Ryther JH (1962) Studies of marine planktonic diatoms. I. Cyclotella nana Hustedt and Detonula confervacea Cleve. Can J Microbiol 8:229–239

    Article  CAS  PubMed  Google Scholar 

  • Guiry M, Cunningham E (1984) Photoperiodic and temperature responses in the reproduction of the north-eastern Atlantic Gigartina acicularis (Rhodophyta: Gigartinales). Phycologia 23:357–367

    Article  Google Scholar 

  • Hall DO, Fernandéz FGA, Guerrero EC, Rao KK, Molina Grima E (2003) Outdoor helical tubular photobioreactors for microalgal production: modelling of fluid-dynamics and mass transfer and assessment of biomass productivity. Biotechnol Bioeng 82:62–73

    Article  CAS  PubMed  Google Scholar 

  • Haugen EM, Cucci TL, Yentsch CM, Shapiro LP (1987) Effects of flow cytometric analysis on morphology and viability of fragile phytoplankton. Appl Environ Microbiol 53:2677–2679

    PubMed Central  CAS  PubMed  Google Scholar 

  • Heaney SI, Jaworski GHM (1977) A simple separation technique for purifying microalgae. Eur J Phycol 12:171–174

    Article  Google Scholar 

  • Janssen M, Tramper J, Mur LR, Wijfells RH (2002) Enclosed outdoor photobioreactors: light regime, photosynthetic efficiency, scale-up, and future prospects. Biotechnol Bioeng 81:193–210

    Article  Google Scholar 

  • Jones AK, Muriel ER, Susan CE (1973) The use of antibiotics to obtain axenic cultures of algae. Br Phycol J 8:185–196

    Article  Google Scholar 

  • Kawai H, Motomura T, Okuda K (2005) Isolation and purification techniques for macroalgae. In: Andersen RA (ed) Algal culturing techniques. Elsevier Academic Press, San Diego, pp 133–144

    Google Scholar 

  • Krauss RW (1962) Mass culture of algae for food and other organic compounds. Am J Bot 49:425–435

    Article  Google Scholar 

  • Li WKW (2002) Macroecological pattern of phytoplankton in the northwestern North Atlantic Ocean. Nature 419:154–157

    Article  CAS  PubMed  Google Scholar 

  • Markham JW, Hasmeier (1982) Observations on the effects of Germanium dioxide on the growth of macroalgae and diatoms. Phycologia 21:125–130

    Article  CAS  Google Scholar 

  • Masanobu K, Mary Héléne N (2005) Sterilization and sterile technique. In: Andersen RA (ed) Algal culturing techniques. Elsevier Academic Press, San Diego, pp 65-81

    Google Scholar 

  • Morel FMM, Rueter JG, Anderson DM, Guillard RRL (1979) Aquil: a chemically defined phytoplankton culture medium for trace metal studies. J Phycol 15:135–141

    Article  CAS  Google Scholar 

  • Perner-Nochta I, Lucumi A, Posten C (2007) Photoautotrophic cell and tissue culture in a tubular photobioreactor. Eng Life Sci 7:127–135

    Article  CAS  Google Scholar 

  • Polne-Fuller M, Gibor A (1984) Developmental studies in Porphyra. I. Blade differentiation in Porphyra perforata as expressed by morphology, enzymatic digestion and protoplast regeneration. J Phycol 20:609–619

    Article  Google Scholar 

  • Price NM, Harrison GI, Hering JG, Hudson RJ, Nirel PMV, Palenik B, Morel FMM (1989) Preparation and chemistry of the artificial algal culture medium aquil. Biol Oceanogr 6:443–461

    Google Scholar 

  • Provasoli L (1968) Media and prospects for the cultivation of marine algae. In: Watanabe A, Hattori A, (eds) Cultures and collections of algae. Proceedings of the U.S.–Japan Conference, Hakone, Japan, September 1966. Japanese Society of Plant Physiology, pp 63–75

    Google Scholar 

  • Provasoli L, Pintner IJ (1960) Artificial media for fresh water algae: problems and suggestions. In: Tyron CA Jr, Hartman RT (eds) The Ecology of algae. Special publication 2. Pymatuning laboratory of Field Biology, University of Pittsburgh, Pittsburgh,Pennsylvania, pp 84–96

    Google Scholar 

  • Richmond A (2003) Handbook of microalgal culture: biotechnology and applied phycology. J Phycol 40:1001–1002

    Google Scholar 

  • Richmond A, Cheng-Wu Z, Zarmi Y (2003) Efficient use of strong light for high photosynthetic productivity: interrelationships between the optical path, the optimal population density and cell-growth inhibition. Biomol Eng 20:229–239

    Article  CAS  PubMed  Google Scholar 

  • Rippka R, Deruelles J, Waterbury JB, Herdman M, Stainer RY (1979) Generic assignments, strain histories and properties of pure cultures of cyanobacteria. J Gen Microbiol 111:1–61

    Google Scholar 

  • Schlösser UG (1994) SAG-Sammlung von Algenkulturen at the University of Göttingen Catalogue of Strains 1994. Bot Acta 107:111–186

    Article  Google Scholar 

  • Sensen CW, Heimann K, Melkonian M (1993) The production of clonal and axenic cultures of microalgae using fluorescence-activated cell sorting. Eur J Phycol 28:93–97

    Article  Google Scholar 

  • Spolaore P, Joannis-Cassan C, Duran E, Isambert A (2006) Commercial applications of microalgae. J Biosci Bioeng 101:87–96

    Article  CAS  PubMed  Google Scholar 

  • Suh IS, Lee SB (2001) Cultivation of a cyanobacterium in an internally radiating air-lift photobioreactor. J Appl Phycol 13:381–388

    Article  Google Scholar 

  • Tatewaki M (1966) Formation of a crustaceous sporophyte with unilocular sporangia in Scytosiphon lomentaria. Phycologia 6:62–66

    Article  Google Scholar 

  • Von Stosch H (1963) Wirkungen von jod un arsenit aufmeeresalgen in kultur. In: De Virville D, Feldmann J (eds) Proceedings of the fourth international seaweed symposium. Pergamon Press, Oxford, pp 142–150

    Google Scholar 

  • Vunjak-Novakovic G, Kim Y, Wu X, Berzin I, Merchhuk JC (2005) Air-lift bioreactors for algal growth on flue gas: mathematical modelling and pilot-plant studies. Ind Eng Chem Res 44:6154–6163

    Article  CAS  Google Scholar 

  • Watanabe MM, Nakagawa M, Katagiri M, Aizawa KI, Hiroki M, Nozaki H (1998) Purification of freshwater picoplanktonic cyanobacteria by pour-plating in ultra-low-gelling-temperature agarose. Phycol Res 46:71–75

    Article  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Marine Biotechnology Laboratory, Department of Botany, University of Delhi, Delhi, 110007, India

    Salam Sonia Devi & Dinabandhu Sahoo

Authors
  1. Salam Sonia Devi
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Dinabandhu Sahoo
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Dinabandhu Sahoo .

Editor information

Editors and Affiliations

  1. Marine Biotechnology Laboratory, Department of Botany, University of Delhi, Delhi, India

    Dinabandhu Sahoo

  2. The Hebrew Univ Jerusalem, Jerusalem, Israel

    Joseph Seckbach

Rights and permissions

Reprints and permissions

Copyright information

© 2015 Springer Science+Business Media Dordrecht

About this chapter

Cite this chapter

Devi, S.S., Sahoo, D. (2015). Culturing Algae. In: Sahoo, D., Seckbach, J. (eds) The Algae World. Cellular Origin, Life in Extreme Habitats and Astrobiology, vol 26. Springer, Dordrecht. https://doi.org/10.1007/978-94-017-7321-8_22

Download citation

Publish with us

Policies and ethics

Search

Navigation