Abstract
The increasing applications of nanotechnology in everyday life require consideration of their interactions with living cells. There are different physical and chemical properties affecting the interactions of nanoparticles with cells, such as NP size, nature of functionalization or stabilizing groups, concentration, and the environment in which nanoparticles are interacting with cells. In this review, we summarize some of our previous studies done on the interactions of gold nanoparticles with supported lipid bilayers (SLB; models for cell membranes). These studies have been done via Quartz Crystal Microbalance with Dissipation (QCM-D) and they include NPs ranging in size from 2 to 40 nm at several concentrations. Their interactions with a SLB composed of l-α-phosphatidylcholine were characterized. In order to better understand how NPs behave in the environment, these interactions were also studied in the presence of different types of natural organic matter (NOM), including Aldrich humic acid, Suwannee River humic acid standard, Suwannee River fulvic acid standard, and Elliot soil humic acid. Here we review our previous findings while focusing on an example of the effect of concentration on NP-SLB interaction.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Barreto JA, O’Malley W, Kubeil M, Graham B, Stephan H, Spiccia L (2011) Nanomaterials: applications in cancer imaging and therapy. Adv Mater (Deerfield Beach, Fla) 23(12):H18–H40. doi:10.1002/adma.201100140
Wang S, Lu W, Tovmachenko O, Rai US, Yu H, Ray PC (2008) Challenge in understanding size and shape dependent toxicity of gold nanomaterials in human skin keratinocytes. Chem Phys Lett 463(1–3):145–149. doi:10.1016/j.cplett.2008.08.039
Yen H-J, Hsu S-H, Tsai C-L (2009) Cytotoxicity and immunological response of gold and silver nanoparticles of different sizes. Small (Weinheim an Der Bergstrasse, Germany) 5(13):1553–1561. doi:10.1002/smll.200900126
Nel AE, Mädler L, Velegol D, Xia T, Hoek EMV, Somasundaran P, Thompson M (2009) Understanding biophysicochemical interactions at the nano-bio interface. Nat Mater 8(7):543–557. doi:10.1038/nmat2442
Tarantola M, Pietuch A, Schneider D, Rother J, Sunnick E, Rosman C, Janshoff A (2011) Toxicity of gold-nanoparticles: synergistic effects of shape and surface functionalization on micromotility of epithelial cells. Nanotoxicology 5(2):254–268. doi:10.3109/17435390.2010.528847
Hou W-C, Moghadam BY, Corredor C, Westerhoff P, Posner JD (2012) Distribution of functionalized gold nanoparticles between water and lipid bilayers as model cell membranes. Environ Sci Technol 46(3):1869–1876. doi:10.1021/es203661k
Pan Y, Neuss S, Leifert A, Fischler M, Wen F, Simon U, Jahnen-Dechent W (2007) Size-dependent cytotoxicity of gold nanoparticles. Small (Weinheim an Der Bergstrasse, Germany) 3(11):1941–1949. doi:10.1002/smll.200700378
Mironava T, Hadjiargyrou M, Simon M, Jurukovski V, Rafailovich MH (2010) Gold nanoparticles cellular toxicity and recovery: effect of size, concentration and exposure time. Nanotoxicology 4(1):120–137. doi:10.3109/17435390903471463
Zhang Y, Chen Y, Westerhoff P, Crittenden J (2009) Impact of natural organic matter and divalent cations on the stability of aqueous nanoparticles. Water Res 43(17):4249–4257. doi:10.1016/j.watres.2009.06.005
Goodman CM, McCusker CD, Yilmaz T, Rotello VM (2004) Toxicity of gold nanoparticles functionalized with cationic and anionic side chains. Bioconjug Chem 15(4):897–900. doi:10.1021/bc049951i
Stankus DP, Lohse SE, Hutchison JE, Nason J (2011) Interactions between natural organic matter and gold nanoparticles stabilized with different organic capping agents. Environ Sci Technol 45(8):3238–3244. doi:10.1021/es102603p
Sutton R, Sposito G (2005) Molecular structure in soil humic substances: the new view. Crit Rev 510:9009–9015
Hardy GJ, Nayak R, Zauscher S (2013) Model cell membranes: techniques to form complex biomimetic supported lipid bilayers via vesicle fusion. Curr Opin Colloid Interface Sci 18(5):448–458. doi:10.1016/j.cocis.2013.06.004
McCubbin GA, Praporski S, Piantavigna S, Knappe D, Hoffmann R, Bowie JH, Martin LL (2011) QCM-D fingerprinting of membrane-active peptides. Eur Biophys J : EBJ 40(4):437–446. doi:10.1007/s00249-010-0652-5
Barenholz Y, Gibbes D, Litman BJ, Goll J, Thompson TE, Carlson RD (1977) A simple method for the preparation of homogeneous phospholipid vesicles. Biochemistry 16(12):2806–2810, Retrieved from http://www.ncbi.nlm.nih.gov/pubmed/889789
Keller CA, Glasmästar K, Zhdanov VP, Kasemo B (2000) Formation of supported membranes from vesicles. Phys Rev Lett 84(23):5443–5446, Retrieved from http://www.ncbi.nlm.nih.gov/pubmed/10990964
Cremer PS, Boxer SG (1999) Formation and spreading of lipid bilayers on planar glass supports. J Phys Chem 291:2554–2559
Mechler A, Praporski S, Atmuri K, Boland M, Separovic F, Martin LL (2007) Specific and selective peptide-membrane interactions revealed using quartz crystal microbalance. Biophys J 93(11):3907–3916. doi:10.1529/biophysj.107.116525
Richter RP, Brisson AR (2005) Following the formation of supported lipid bilayers on mica: a study combining AFM, QCM-D, and ellipsometry. Biophys J 88(5):3422–3433. doi:10.1529/biophysj.104.053728
Dixon MC (2008) Quartz crystal microbalance with dissipation monitoring: enabling real-time characterization of biological materials and their interactions. J Biomol Tech 19:151–158
Furman O, Usenko S, Lau BLT (2013) Relative importance of the humic and fulvic fractions of natural organic matter in the aggregation and deposition of silver nanoparticles. Environ Sci Technol 47(3):1349–1356. doi:10.1021/es303275g
Vogt BD, Lin EK, Wu W, White CC (2004) Effect of film thickness on the validity of the Sauerbrey equation for hydrated polyelectrolyte films. J Phys Chem B 108:12685–12690
Gottschalk F, Nowack B (2011) The release of engineered nanomaterials to the environment. J Environ Monit: JEM 13(5):1145–1155. doi:10.1039/c0em00547a
Lewinski N, Colvin V, Drezek R (2008) Cytotoxicity of nanoparticles. Small (Weinheim an Der Bergstrasse, Germany) 4(1):26–49. doi:10.1002/smll.200700595
Thomas CR, George S, Horst AM, Ji Z, Miller RJ, Peralta-Videa JR, Zink JI (2011) Nanomaterials in the environment: from materials to high-throughput screening to organisms. ACS Nano 5(1):13–20. doi:10.1021/nn1034857
Soenen SJ, Manshian B, Montenegro JM, Amin F, Meermann B, Thiron T, Cornelissen M, Vanhaecke F, Doak S, Parak WJ, De Smedt S, Braeckmans K (2012) Cytotoxic effects of gold nanoparticles: A multiparametric study. ACS Nano 7:5767–5783
Lesniak A, Salvati A, Santos-Martinez MJ, Radomski MW, Dawson K, Åberg C (2013) Nanoparticle adhesion to the cell membrane and its effect on nanoparticle uptake efficiency. J Am Chem Soc 135(4):1438–1444. doi:10.1021/ja309812z
Acknowledgements
This work was supported in part by the National Science Foundation (CBET 0966496). For help with concentration experiments, the authors thank Jeniece Macedonio for her contribution.
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2015 Springer Science+Business Media Dordrecht
About this paper
Cite this paper
Kamaloo, E., Bailey, C., Camesano, T.A. (2015). Effect of Concentration on the Interactions of Gold Nanoparticles with Model Cell Membranes: A QCM-D Study. In: Camesano, T. (eds) Nanotechnology to Aid Chemical and Biological Defense. NATO Science for Peace and Security Series A: Chemistry and Biology. Springer, Dordrecht. https://doi.org/10.1007/978-94-017-7218-1_5
Download citation
DOI: https://doi.org/10.1007/978-94-017-7218-1_5
Publisher Name: Springer, Dordrecht
Print ISBN: 978-94-017-7217-4
Online ISBN: 978-94-017-7218-1
eBook Packages: Chemistry and Materials ScienceChemistry and Material Science (R0)