Abstract
Since its discovery (Duysens, 1954) the light-induced absorbance change in the 480–550 nm wavelength region, with its characteristic difference spectrum, has been studied in much detail. Ample evidence has been presented (e.g. Junge, 1977) that this so-called P515 absorbance change is due to an electrochromic band shift of a pigment moiety with a maximum around 515 nm. This has made it a useful intrinsic probe for studying the magnitude and kinetics of formation and decay of the transmembrane electric potential of the inner chloroplast (thylakoids) membranes. There has been found reasonable good agreement on the value of the initial potential generated by saturating single turnover light flashes, measured directly by micro-electrodes and calculated by calibrating the absorbance change caused by salt-induced diffusion potentials (e.g. Vredenberg, 1981). The kinetics of P515 responses in continuous light as well as in single turnover flashes are however at variance with the responses measured with microelectrodes. There is now general agreement, based on observations in intact cells as well as in intact and broken chloroplasts that the P515 absorbance change in single turnover flashes is a multiphasic phenomenon, reflected by a biphasic rise and a decay which consists of at least three different single exponentials.
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References
Duysens LNM (1954) Reversible changes in the absorption spectrum of Chlorella upon irradiation, Science 120, 353–354.
Hong YQ, Junge W (1983) Localized or delocalized protons in phosphorylation. On the accessibility of the thylakoid lumen for ions and buffers, Biochim. Biophys. Acta 722, 197–208.
Junge W (1977) Membrane potentials in photosynthesis, Annu. Rev. Plant Physiol. 28, 503–536.
van Kooten O, Gloudemans AGM and Vredenberg WJ (1983) On the slow component of P515 and the flash induced reduction of cyt b563 in chloroplasts, Photobiochem. Photobiophys. 6(1), 9–14.
Murphy DJ (1982) The importance of non-planar bilayer regions in photosynthetic membranes and their stabilisation by galactolipids, FEBS Letters 150(1), 19–26.
Peters RLA, Bossen M, van Kooten O and Vredenberg WJ (1983) On the correlation between the activity of the ATP-hydrolase and the kinetics of the flash-induced P515 electrochromic bandshift in chloroplasts, J. Bioenerg. Biomembr. (in press).
Schapendonk AHCM, Vredenberg WJ and Tonk WJM (1979) Studies on the kinetics of the 515 nm absorbance changes in chloroplasts, FEBS Letters 100, 325–330.
Schapendonk AHCM (1980) Electrical events associated with primary photo-synthetic reactions in chloroplast membranes, Agricultural Research Reports 905, ISBN 90 220 0756 1, Wageningen.
Schreiber U and Rienits KG (1982) Complimentarity of ATP-induced absorbance changes around 515 nm, Biochim. Biophys. Acta 682, 115–213.
Schuurmans JJ, Peters ALJ, Leeuwerik FJ and Kraayenhof R (1981) On the association of electrical events with the synthesis and hydrolysis of ATP in photosynthetic membranes. In Palmiere F et al., ed. Vectorial reactions in electron and ion transport in mitochondria and bacteria, pp. 359–369, Elsevier, Amsterdam.
Selak MA and Whitmarsh J (1982) Kinetics of the electrogenic step and cytochrome b6 and f redox changes in chloroplasts, FEBS Letters 150, 286–292.
Vredenberg WJ (1981) P515: a monitor of photosynthetic energization in chloroplast membranes, Physiol. Plant. 53, 598–602.
Westerhof HV, Helgerson SL, Theg SM, van Kooten O, Wikstrom MFK, Skulachev VP, Dancshazy Zs (1983) The present state of the chemiosmotic theory, Acta Biol. Acad. Sci. Hung. (in press).
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© 1984 Springer Science+Business Media Dordrecht
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Vredenberg, W.J., Van Kooten, O., Peters, R.L.A. (1984). Electrical Events and P515 Response in Thylakoid Membranes. In: Sybesma, C. (eds) Advances in Photosynthesis Research. Advances in Agricultural Biotechnology, vol 2. Springer, Dordrecht. https://doi.org/10.1007/978-94-017-6368-4_54
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DOI: https://doi.org/10.1007/978-94-017-6368-4_54
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