Abstract
Green photosynthetic sulfur bacteria have an extensive antenna system consisting of 1000 – 2000 bacteriochlorophyll (BChl) molecules per reaction center. Most of these are BChl c, d or e, contained in the so-called chlorosomes. BChl a is situated in or close to the cytoplasmic membrane, which also contains the reaction center (Olson, 1978). Incubation of the membrane with Triton X-100 produces a pigment-protein complex (PP), that contains about 80 BChl a. per reaction center. Subsequent treatment with guanidine-HCl removes two molecules of the light-harvesting BChl a protein, each containing 21 BChl a and produces the RCPP complex (about 35 BChl a; Swarthoff, Amesz, 1979). Fluorescence emission and excitation and linear dichroism (LD) spectra indicate that the RCPP complex still contains a significant amount of BChl a protein in a bound form, in addition to a ‘core complex’, that is associated with the reaction center (Kramer et al., 1982; Swarthoff et al., 1980). In this paper we describe the isolation of this core complex and some of its properties.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
Kramer HJM, Kingma H, Swarthoff T and Amesz J (1982) Prompt and delayed fluorescence in pigment-protein complexes of a green photosynthetic bacterium, Biochim. Biophys. Acta 681, 359–364.
Olson JM (1978) Bacteriochlorophyll a-proteins from green bacteria. In Clayton RK and Sistrom WR, eds. The Photosynthetic Bacteria, pp. 161–178. New York-London: Plenum Press.
Philipson KD and Sauer K (1973) Exciton interaction in a bacteriochlorophyll-protein from Chloropseudomonas ethylica. Absorption and circular dichroism at 77 degrees K, Biochemistry 11, 1880–1885.
Swarthoff T and Amesz J (1979) Photochemically active pigment-protein complexes from the green photosynthetic bacterium Prosthecochloris aestuarii, Biochim. Biophys. Acta 548, 427–432.
Swarthoff T, de Grooth BG, Meiburg RF, Rijgersberg CP and Amesz J (1980) Orientation of pigments and pigment-protein complexes in the green photosynthetic bacterium Prosthecochloris aestuarii, Biochim. Biophys. Acta 593, 51–59.
Swarthoff T, Amesz J, Kramer HJM and Rijgersberg CP (1981) The reaction center and antenna pigments of green photosynthetic bacteria, Israel J. Chem. 21, 332–337.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1984 Springer Science+Business Media Dordrecht
About this chapter
Cite this chapter
Vasmel, H., Swarthoff, T., Kramer, H.J.M., Amesz, J. (1984). A Light-Harvesting Pigment-Protein Complex Associated with the Reaction Center of Green Bacteria. In: Sybesma, C. (eds) Advances in Photosynthesis Research. Advances in Agricultural Biotechnology, vol 2. Springer, Dordrecht. https://doi.org/10.1007/978-94-017-6368-4_42
Download citation
DOI: https://doi.org/10.1007/978-94-017-6368-4_42
Publisher Name: Springer, Dordrecht
Print ISBN: 978-90-247-2943-2
Online ISBN: 978-94-017-6368-4
eBook Packages: Springer Book Archive