Summary
Iso-immune precipitins have been produced using a water-oil-water (WOW) type of emulsion adjuvant incorporating whole pig serum. The reactions are demonstrated by double diffusion (Ouchterlony) tests on microscope slides.
The characteristics of the antibodies and antigens involved have been analysed using immuno-electrophoresis (I.E.), specific staining techniques, exclusion chromatography (molecular sieving) and ion exchange fractionation techniques. Antigens have been demonstrated with α, β and γ mobilities, and to date they all fractionate in the middle (7S) peak” of Sephadex G200. The γ characteristic fractionates as IgG on anion exchange chromatography and demonstrate IgG identity in other ways.
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References
Bailleux, R. E., Bernier, G. M., Tominaga, K. and Putnam, F. W., 1964. Gamma globulin antigenic types defined by heavy chain determinants, Science, 145, 168.
Baumstark, J. S., Laffin, R. J. and Bardawil, W. A., 1964. A preparative method for the separation of 7S gamma globulin from human serum, Arch. Biochem. Biophys., 108,3, 514–521.
Dray, S., Dubiski, S., Kelus, A., Lennox, E. S. and Oudin, J., 1962. A notation for allotypes, Nature, 195, 785.
Feinstein, A., 1963. Character and allotype and immune globulin in rabbit colostrum, Nature, 199, 1197.
Feinstein, A., Gell, P. G. H. and Kelus, A. S., 1963. Immimochemical analysis of rabbit gamma globulin allotypes, Nature, 200, 653.
Flodin, P., 1965 Methodological aspects of gel filtration with special reference to desalting operations, J. Chromat., 5, 103.
Fudenberg, H. H., Heremans, J. F. and Franklin, E. C, 1963. A hypothesis for the genetic control of synthesis of the gamma-globulin, Ann. Inst. Past., 104, 155.
Grey, H. M. and Kunkel, H. G, 1964. H chain subgroups of myeloma proteins and normal 7S gamma-globulin, J. Exp. Med., 120, 253.
Herbert, W. J., 1965. Multiple emulsions — A new form of mineral oil antigen adjuvant, Lancet, ii, 771.
Hirschfeld, J., I1960. Immuno-electrophoresis — Procedure and application to the study of group specific variation in sera, Science Tools,1, 18–25.
Kelus, A. S. and Gell, P. G. H., 1965. An allotypic determinant specific to rabbit macroglobulin, Nature, 206, 313.
Killander, J. and Flodin, P., 1962, The fractionation of serum proteins by gel filtration, Vox. Sang., 1, 113.
Levy, H. B. and Sober, H. A., 1960. A simple chromatographic method for preparation of gamma globulin, Proc. Soc. Expl. Biol. Med., 103, 250.
Martensson, L., 1966. Genes and immunoglobulin — Editorial, Vox. Sang., 11. 521–545.
Porath, J. and Bennich, H., 1962. Recycling chromatography, Arch. Biochem. Biophys. (Suppl.),1, 152–156.
Rasmusen, B. A., 1965. Iso-antigens of gamma globulins in pigs, Science, 148, 1742.
Sell, S., 1966. Immunoglobulin M allotypes of the rabbit: identification of a second specificity, Science, 153, 641.
Sober, H. A., Gutter, F. J., Wyckoff, M. and Peterson, E. A., 1956. Chromatography of proteins. II. Fractionation of serum proteins on anion-exchange cellulose, J. Amer. Chem. Soc, 78.,756.
Terry, W. D. and Fahey, J. L., 1964. Subclasses of human gamma 2 globulin based on differences in the heavy polypeptide chains, Science, 146, 400.
Todd, C. W., 1963. Allotypes in rabbit 19S protein, Biochem. Biophys. Res. Comm., 11, 170.
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© 1970 Springer Science+Business Media Dordrecht
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Lang, B.G. (1970). Globulin Allotyping in Pigs Using Iso-precipitins. In: XIth European Conference on Animal Blood Groups and Biochemical Polymorphism. Springer, Dordrecht. https://doi.org/10.1007/978-94-017-5226-8_45
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DOI: https://doi.org/10.1007/978-94-017-5226-8_45
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