Abstract
When the hybridoma technique (Köhler, Milstein 1975) is employed, purification of the antigen prior to immunization is not necessary to obtain monospecific antibodies, so called monoclonal antibodies. In the present paper a method is described which produces in high yield monoclonal antibodies to chlorophyll a-protein 1 (Chl a -P1) a component of photosystem I (PSI) vesicles (Møller et al. 1981). Exploitation of this method offers an additional tool in our quest of identifying the function of the barley thylakoid polypeptides (see von Wettstein, these proceedings).
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
Burnette WN (1981) “Western blotting”: Electrophoretic transfer of proteins from sodium dodecyl sulfate-polyacrylamide gels to unmodified nitrocellulose and radiographie detection with antibody and radioiodinated Protein A, Anal. Biochem. 112, 195–203.
Köhler G and Milstein C (1975) Continuous cultures of fused cells secreting antibody of predefined specificity, Nature 256, 495–497.
Machold O, Simpson J and Høyer-Hansen G (1977) Correlation between the freeze fracture appearance and polypeptide composition of thylakoid membranes in barley, Carlsberg Res. Commun. 42, 499–516.
Møller, BL, Høyer-Hansen G and Hiller RG (1981) Functional identification of barley thylakoid polypeptides resolved by SDS-polyacrylamide gel electrophoresis. In Akoyunoglou G, ed. Photosynthesis III Structure and molecular organization of the photosynthetic apparatus, pp 245–256. Philadelphia: Balaban Int. Sci. Ser.
Rott R and Nelson N (1981) Purification and immunological properties of proton-ATPase complexes from yeast and rat liver mitochondria, J. Biol. Chem. 256, 9224–9228.
Schreier M, Köhler G, Hengartner H, Berek C, Trucco M, Forni L, Staehelin T Stocker J and Takacs B (1980) Hybridoma techniques, EMBO, SKMB Course 1980, Basel.
Stähli C, Staehelin T, Miggiano V, Schmidt J and Härina P (1980) High frequences of antigen-specific hybridomas: Dependence on immunization parameters and Prediction by Spleen cell analysis, J. Immunol. Meth. 32, 297–304.
Towbin H, Staehelin T and Gordon J (1979) Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: Procedure and some applications, Proc. Nat. Acad. Sci. USA 76, 4350–4354.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1984 Springer Science+Business Media Dordrecht
About this chapter
Cite this chapter
Høyer-Hansen, G. (1984). Monoclonal Antibodies to Chlorophyll α-protein 1 in Barley. In: Sybesma, C. (eds) Advances in Photosynthesis Research. Advances in Agricultural Biotechnology, vol 3. Springer, Dordrecht. https://doi.org/10.1007/978-94-017-4973-2_37
Download citation
DOI: https://doi.org/10.1007/978-94-017-4973-2_37
Publisher Name: Springer, Dordrecht
Print ISBN: 978-90-247-2944-9
Online ISBN: 978-94-017-4973-2
eBook Packages: Springer Book Archive