Abstract
Wheat-barley hybrids were successfully produced in the following combinations: CS × Betzes, Mv9 krl × Igri, Mv9 krl × Osnova and Asakazekomugi × Manas. The hybrids were multiplied in tissue culture because of the high degree of sterility.
Meiotic chromosome pairing was analysed with the Feulgen technique and genomic in situ hybridization (GISH) in the hybrids and regenerants. Chiasma frequency greatly increased after in vitro culture in regenerants from the hybrids. Wheat-barley chromosome pairing was detected by genomic in situ hybridization in the hybrids and regenerants. The demonstration of wheat-barley chromosome pairing by GISH confirms the possibility of creating recombinants from these crosses.
The regenerants were pollinated with wheat to develop backcross progenies from the CS × Betzes and the Mv9 kr1 × Igri combinations. The selection of disomic additions from the selfed (Mv9 krl × Igri) Mv9 kr12 plants is now in progress.
Five translocated wheat-barley chromosomes were recovered from 51 BC2F2 progeny of the in vitro regenerated CS × Betzes hybrids. Following selfing, homozygotes of four translocations were selected. Identification of barley segments involved in these translocations using SSR markers have been started. The experiment suggests that in vitro culture conditions are conducive to major genome rearrangements in wheat-barley hybrids.
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Molnár-Láng, M., Linc, G., Nagy, E.D. (2001). Production and Molecular Cytogenetic Analysis of Wheat-Barley Hybrids and Translocations. In: Bedö, Z., Láng, L. (eds) Wheat in a Global Environment. Developments in Plant Breeding, vol 9. Springer, Dordrecht. https://doi.org/10.1007/978-94-017-3674-9_96
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DOI: https://doi.org/10.1007/978-94-017-3674-9_96
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