Abstract
A linkage map of the Ixodes scapularis genome was constructed based upon segregation amongst 127 loci. These included 84 random amplified polymorphic DNA (RAPD) markers, 32 Sequence-Tagged RAPD (STAR) markers, 5 cDNAs, and 5 microsatellites in 232 Fl intercross progeny from a single, field-collected Pl female. A preliminary linkage map of 616 cM was generated across 14 linkage groups with one marker every 10.8 cM. Assuming a genome size of ~109 bp, the relationship of physical to genetic distance is ~300 kb/cM in the I. scapularis genome.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
Antolin, M.F., Bosio, C.F.. Cotton. J.. Sweeney, W., Strand, M.R. and Black, W.C.t. 1996. Intensive linkage mapping in a wasp (Bracon hebetor) and a mosquito (Aedes aegypti) with single-strand conformation polymorphism analysis of random amplified polymorphic DNA markers. Genetics 143: 1727–1738.
Black, W.C. and DuTeau. N.M. 1997. In: The Molecular Biology of Insect Disease Vectors: A Methods Manual, pp. 361–373. Chapman Hall, New York.
Bosio, C.F., Fulton, R.E., Salasek, M.L., Beaty. B.J. and Black. W.C.t. 2000. Quantitative trait loci that control vector competence for dengue-2 virus in the mosquito Aedes aegypti. Genetics 156: 687–698.
Brown, S.E., Severson, D.W., Smith, L.A. and Knudson, D.L. 2001. Integration of the Aedes aegypti mosquito genetic linkage and physical maps. Genetics 157: 1299–1305.
Dietrich, W., Katz, H., Lincoln, S.E., Shin, H.S., Friedman, J.. Dracopoli, N.C. and Lander, E.S. 1992. A genetic map of the mouse suitable for typing intraspecific crosses. Genetics 131: 423–447.
Dimopoulos, G., Zheng, L., Kumar, V., della Torre, A.. Kafatos, F.C. and Louis, C. 1996. Integrated genetic map of Anopheles gambiae: use of RAPD polymorphisms for genetic, cytogenetic and STS landmarks. Genetics 143: 953–960.
Fagerberg, A.J., Fulton, R.E. and Black, W.C. 2001. Microsatellite loci are not abundant in all arthropod genomes: analyses in the hard tick, (codes scapularis and the yellow fever mosquito, Aedes aegypti. Insect Mol. Biol. 10: 225–236.
Fulton, R.E., Salasek, M.L., DuTeau, N.M. and Black. W.C.t. 2001. SSCP analysis of cDNA markers provides a dense linkage map of the Aedes aegypti genome. Genetics 158: 715–726.
Hayashi, K. 1991. PCR-SSCP: a simple and sensitive method for detection of mutations in the genomic DNA. PCR Meth. Appl. 1: 34–38.
Hiss, R.H., Norris, D.E., Dietrich, C.H.. Whitcomb, R.F., West, D.F., Bosio, C.F., Kambhampati, S., Piesman, J., Antolin, M.F. and Black. W.C.t. 1994. Molecular taxonomy using single-strand conformation polymorphism (SSCP) analysis of mitochondrial ribosomal DNA genes. Insect Mol. Biol. 3: 171–182.
Hudson, T.J., Stein, L.D., Gerety, S.S., Ma. J.. Castle, A.B., Silva. J., Slonim. D.K., Baptista, R., Kruglyak, L., Xu, S.H. et al. 1995. An STS-based map of the human genome. Science 270: 1945–1954.
Hunt, G.J. and Page Jr., R.E. 1995. Linkage map of the honey bee. Apis mellifera. based on RAPD markers. Genetics 139: 1371–1382.
Kosambi, D.D. 1944. The estimation of map distances from recombination values. Ann. Eugen. 12: 172–175.
Oliver Jr., J.H. 1977. Cytogenetics of mites and ticks. Annu. Rev. Entomol. 22: 407–429.
Orita, M., Suzuki, Y., Sekiya, T. and Hayashi, K. 1989. Rapid and sensitive detection of point mutations and DNA polymorphisms using the polymerase chain reaction. Genomics 5: 874–879.
Palmer, M.J., Bantle, J.A., Guo, X. and Fargo, W.S. 1994. Genome size and organization in the ixodid tickAmblyomma americanum (L.). Insect Mol. Biol. 3: 57–62.
Sonenshine, D.E. 1991. Biology of Ticks. Vol. I. New York.
Spielman, A. 1976. Human babesiosis on Nantucket Island: transmission by nymphal Ixodes ticks. Am. J. Trop. Med. Hyg. 25: 784–787.
Spielman, A., Wilson, M.L., Levine, J.F. and Piesman, J. 1985. Ecology of (rodes damminiborne human babesiosis and Lyme disease. Annu. Rev. Entomol. 30: 439–460.
Stam, P. and van Oojin, J.W. 1995. CPRO-DLO, Wageningen. The Netherlands.
Telford III, S.R., Dawson, J.E., Katavolos, P., Warner, C.K., Kolbert, C.P. and Persing, D.H. 1996. Perpetuation of the agent of human granulocytic ehrlichiosis in a deer tick-rodent cycle. Proc. Natl. Acad. Sci. USA 93: 6209–6214.
Ullmann. A.J., Piesman, J., Dolan, M.C. and Iv, W.C. 2003. A preliminary linkage map of the hard tick, Ixodes scapularis. Insect Mol. Biol. 12: 201–210.
van Ooijen, J.W. 1994. DrawMap: a computer program for drawing genetic linkage maps. J. Hered. 85: 66.
Vidal-Puig, A. and Moller, D.E. 1994. Comparative sensitivity of alternative single-strand conformation polymorphism (SSCP) methods. Biotechniques 17: 490–492, 494, 496.
Williams, J.G., Kubelik, A.R., Livak, K.J., Rafalski, J.A. and Tingey, S.V. 1990. DNA polymorphisms amplified by arbitrary primers are useful as genetic markers. Nucl. Acids Res. 18: 6531–6535.
Yasukochi, Y. 1998. A dense genetic map of the silkworm. Bombyx mori, covering all chromosomes based on 1018 molecular markers. Genetics 150: 1513–1525.
Zheng, L., Benedict. M.Q., Cornel. A.J., Collins, F.H. and Kafatos, F.C. 1996. An integrated genetic map of the African human malaria vector mosquito, Anopheles gambiae. Genetics 143: 941–952.
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2003 Springer Science+Business Media Dordrecht
About this chapter
Cite this chapter
Ullmann, A.J., Piesman, J., Dolan, M.C., Black, W.C. (2003). A preliminary linkage map of the tick, Ixodes scapularis . In: Jongejan, F., Kaufman, W.R. (eds) Ticks and Tick-Borne Pathogens. Springer, Dordrecht. https://doi.org/10.1007/978-94-017-3526-1_8
Download citation
DOI: https://doi.org/10.1007/978-94-017-3526-1_8
Publisher Name: Springer, Dordrecht
Print ISBN: 978-90-481-6355-7
Online ISBN: 978-94-017-3526-1
eBook Packages: Springer Book Archive