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Thin Cell Layer Concept

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Thin Cell Layer Culture System: Regeneration and Transformation Applications

Abstract

The understanding of the mechanisms which control plant growth and development is of fundamental and applied interests. In order to analyze these mechanisms, we need to be able to program in vitro a given pattern of differentiation before trying to answer to the question of how it is programmed in vitro i.e. in the plant body.

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References

  • Ahn, I.O., Bui Van Le., Gendy, C., and K. Tran Thanh Van (1996) Direct somatic embryogenesis through thin cell layer culture in Panax ginseng, Plant Cell Dss.Org Cult 45, 237–243.

    CAS  Google Scholar 

  • Altamura, M.M, Torrigiani, P., Capittanni, F., Scaramagli, S. and Bagni, N. (1991) De novo root formation in tobacco thin layers is affected by inhibition of polyamine biosynthesis, J Exp. Bot 42, 1575–1582.

    CAS  Google Scholar 

  • Ammirato, P.H.V. (1987) Speeding transgenic plants, Biotechnology 5, 1015.

    Google Scholar 

  • Appelbaum, A., Canellakis, Z.N., Applewhite, B., Kaur-Sawhney, R. and Galston, A.W. (1988) Binding of spermidine to a unique protein in thin cell layer tobacco tissue culture, Plant Physiol 88, 996–998.

    Article  Google Scholar 

  • Becher, T., Haberland. and Koop, H.U, (1992) Callus formation and plant regeneration in standard and microexplants from seedling of barley (Hordeum vulgare L.), Plant Cell Rep 11, 39–43.

    Google Scholar 

  • Biondi, S., Scaramagli, S., Capitani, F., Altamura, M.M. and Torrigiani, R. (2001) Methyl jasmonate upregulates biosynthetic gene expression, oxidation and conjugation of polyamines and inhibits shoot formation in tobacco thin layers, J Exp. Bot 52, 231–242.

    Article  PubMed  CAS  Google Scholar 

  • Blake, J. (1983) Tissue culture propagation of coconut, date and oil palm, In: Dodds, J.H. (Ed.) Tissue culture of tree, Croom Helm, Lodon, pp. 29–50.

    Chapter  Google Scholar 

  • Bui, V. L., Nghieng Thao, D. M., Gendy, C., Vidal, J. and Tran Thanh Van, K. (1997) Somatic embryogenesis on thin cell layers of a C4 species, Digitaria sanguinalis (L.) Scop, Plant Cell Tiss. Org . Cult 49, 201–208.

    Article  Google Scholar 

  • Bui, V. L., Nghieng Thao, D. M., Jeanneau, M., Sadik, S., Shanjun, T., Vidal, J. and Tran Thanh Van, K. (1998a) Rapid plant regeneration of a C4 dicot species: Amaranthus edulis, Plant Sei 132, 45–54.

    Article  Google Scholar 

  • Bui, V. L., Nghieng Thao, D.M., Jeanneau, M., Sadik, S., Shanjun, T., Vidal, J. and Tran Thanh Van, K. (1998b) Rapid regeneration of whole plants in large crabgrass (Digitaria sanguinalis L.) using thin cell layer culture, Plant Cell Rep 18, 166–172.

    Article  Google Scholar 

  • Bui, V L., Nghieng Thao, D. M., Jeanneau, M., Sadik, S., Shanjun, T., Vidal, J. and Tran Thanh Van, K. (1998c) Transformation of a C4 monocot, the Digitaria sanguinalis (Large crabgrass) using somatic embryogenesis induced on thin cell layers. 9th Irrt Cong Plant Tiss Cult. Jerusalem, Israel, 14–19 June. Abst pp. 1911

    Google Scholar 

  • Bui, V. L., Nhut, D. T and Tran Thanh Van, K. (1999a) Plant production via shoot regeneration from thin cell layer pseudo-bulblet explants of Lilium longiflorum in vitro, C. R. Acad. Sci. Paris 322, 303–310.

    Article  Google Scholar 

  • Bui, V.L., Ha, N.T., Anil Hong, L.T. and Tran Thanh Van, K. (1999b) High frequency shoot regeneration from trifoliate orange (Poncitrus trifoliate L. Raf) using thin cell layer method, Comptes Rendus de l’Académie des Sciences 322, 1105–1111.

    Google Scholar 

  • Bui, V.L., Hang Phuong, N.T., Anh Hong, L.T. and Tran Thanh van, K. (1999e) High frequency shoot regeneration from Rhynchostylis gigantea (orchidaceae) using thin cell layers, Plant Growth Reg 28, 179–185.

    Article  Google Scholar 

  • Campbell, K.H.S., McWhir, J., Ritchie, W.A. and Wilmut, I. (1996) Sheep cloned by nuclear transfer from a cultured cell line, Nature 380, 64–66.

    Article  PubMed  CAS  Google Scholar 

  • Carimi, F., De-Pasquale, F. and Crescimanno, F.G. (1999) Somatic embryogenesis and plant regeneration from pistil thin cell layers of Citms, Plant Cell Rep 18, 935–940.

    Article  CAS  Google Scholar 

  • Charest, P.J., Holbrook, L.A., Gabard, J., Iyer, V.N. and Miki, B.L. (1988) Agrobacterium-mediatedtransformation of thin cell layer explants from Brassica napus L., Theor Appl Genet 75, 438–445.

    Google Scholar 

  • Chlyah, A. (1974) Inter-tissue correlations in organ fragments, organogenetic capacity of tissue excised from stem segments of Torenia fournieri L. cultured separately in vitro, Plant Physiol 54, 341–348.

    Article  PubMed  CAS  Google Scholar 

  • Compton, M.E. and Veilleux, R.E. (1992) Thin cell layer morphogenesis, Hort. Rev 14, 239–264

    Google Scholar 

  • Conger. B.V., Novak, F.J., Afza, R. and Erdelsky, K. (1987) Somatic embryogenesis from cultured leaf segments of Zea mays, Plant Cell Rep 6, 345–347.

    Article  Google Scholar 

  • Creemers-Molenaars, J., Harkkert, J.C., Van Staveren, M.J. and Gilissen, L.J.W. (1994) Histology of the morphogenic response in thin cell layer explants from vegetative tobacco plants, Ann Bot 73, 547–555.

    Article  Google Scholar 

  • Cruz-de-Carvalho-Maria, I-I., Bui, V.L., Zuily-Fodil, Y., Pham Thi, A.T. and Tran Thanh Van, K. (2000) Efficient whole plant regeneration of common bean (Phaseolus vulgaris L.) using thin cell layer culture and silver nitrate, Plant Sci 159, 223–232.

    Article  Google Scholar 

  • Das, A.B., Rout, G.R. and Das, P. (1995) In vitro somatic embryogenesis from callus culture of the timber yielding tree Hardwickia binata Roxb, Plant Cell Rep 15, 147–149.

    CAS  Google Scholar 

  • De Vries, S.C., Booiji, H., Janssens, R., Vogels, R., Saris, L., Terzi, M. and Van Kammen, A. (1988) Carrot somatic embryogenesis depends on the phytohormone controlled presence of correctly glycosylated extracellular proteins. Gene Dev 2, 462–476.

    Article  Google Scholar 

  • Detrez, C., Tetu, T. Sangwan, R.S. and Sangwan-Norrel, B.S. (1988) Direct organogenesis from petiole and thin cell layer explants in sugar beet cultured in vitro, JExp Bot 39, 917–926.

    CAS  Google Scholar 

  • Dien, N.T. and Tran Thanh Van, K. (1974) Differenciation in vitro et de novo d’organes floraux directement partir des couches minces de cellules de type epidermique de Nicotiana tabacum. Etude au niveau cellulaire, Can. J. Bot 52, 2319–2322.

    Article  Google Scholar 

  • Eberhard, S., Doubrava, N., Marf, V, Mohnen, D., Southwick, A. and Albersheim, P. (1989) Pectic cell wall fragments regulate tobacco thin cell layer explant morphogenesis, The Plant Cell 1, 747–755.

    PubMed  CAS  Google Scholar 

  • Fu, Y.F., Han, Y.Z., Zhao, D.G. and Meng, F.J. (2000) Zearalenone and flower bud formation in thin cell layers of Nicotiana tabacum L., Plant Growth Reg 30, 271–274.

    Article  CAS  Google Scholar 

  • Gendy, C., Sene, M., Bui V.L., Vidal, J. and Tran Thanh Van, K. (1996) Somatic embryogenesis and plant regeneration in Sorghum bicolor (L.) Moench, Plant Cell Rep 15, 900–904.

    Article  CAS  Google Scholar 

  • Gill, R., Gerrath, J. and Saxena, P.K. (1992) High frequency direct embryogenesis in thin cell layer cultures of hybrid seed geranium (Pelargonium), Can. J. Bot 71, 408–413.

    Article  Google Scholar 

  • Goh, C.J., Lakshmanan, P. and Loh, C.S. (1994) High frequency direct shoot bud regeneration from excised leaves of mangosteen (Garcinia mangostana L.), Plant Sci 101, 173–180.

    Article  CAS  Google Scholar 

  • Goh, C.J., Nathan, M.J. and Kumar, P.P. (1995) Direct organogenesis and induction of morphogenic callus through thin section culture of Heliconia psittacorum, Sci Hort 62, 113–120.

    Article  Google Scholar 

  • Gupta, P.K., Kendurka, S.V., Kulkarni, V.N., Shigurka, M.V. and Mascarenhas, A.F. (1984) Somatic embryogenesis and plants from zygotic embryos of coconut (Cocos nucifera L.) in vitro, Plant Cell Rep 3, 222–225.

    Article  Google Scholar 

  • Hicks, G.S. (1981) Patterns of organ development in plant tissue culture and the problem of organ determination, Bot. Rev 46, 1–23.

    Article  Google Scholar 

  • Jeanneau, M., Bui, V.L., Do My, N.T., Vidal, J. and Tran Thanh Van, K. (2000) Transformation techniques of Amaranthus using thin cell layers, Third International Workshop in Plant Biotechnology, Hanoi 19–25, pp. 16.

    Google Scholar 

  • Jullien, F. and Wyndaele, R. (1992) Precocious in vitro flowering of soybean cotyledonary node, J. Plant Physiol 140, 251–253.

    Article  CAS  Google Scholar 

  • Juillien, F. and Tran Thanh Van, K. (1994) Micropropagation and embiyoid formation from young leaves of Bambusa glaucescens “Golden Goddess”, Plant Sci 98, 199–207.

    Article  Google Scholar 

  • Kay, L.E., and Basile, D.V. (1987) Specific peroxidase isoenzymes are correlated with organogenesis, Plant Physiol 84, 99–105.

    Article  PubMed  CAS  Google Scholar 

  • Krismaszewska, K. and Keller, W.A. (1985) High frequency plant regeneration from thin cell layer explants of Brassica napus, Plant Cell Tiss. Org. Cult 4, 183–197.

    Article  Google Scholar 

  • Lakshmanan, P., Loh, C.S. and Goh, C.J. (1995) An in vitro method for rapid regeneration of a monopodial orchid hybrid Aranda Deborah using thin section culture, Plant Cell Rep 14, 510–514.

    Article  CAS  Google Scholar 

  • Le Kiem Ngoc-Tran Thanh Van. (1965) Vernalisation du Genus urbanum L. Masson et Cie Paris, pp. 593. Lee-Stadlemann, O.Y., Lee, S., Hackett, W.P. and Read, P.E. (1989) The formation of adventitious buds in vitro on micro-sections of hybrid Populus leaf mid veins, Plant Sci 61, 263–272.

    Google Scholar 

  • Lerouge, P., Roche, P., Faucher, C., Maillet, F., Truchet, G., Prome, J.C. and Denarie, J. (1990) Symbiotic host-specificity of Rhizobium meliloti is determined by a sulphated and acylated glucosamine oligosaccharide signal, Nature 344, 781–784.

    Article  PubMed  CAS  Google Scholar 

  • Marfa, V, Gollin, D., Eberhard, S., Mohnen, D., Darvill, A. and Albersheim, P. (1991) Oligogalacturonides are able to induce flowers to form on tobacco explants, Plant J 1, 217–225.

    Google Scholar 

  • Meeks-Wagner, D., Dennis, E., Tran Thanh Van, K. and Peacock, W. (1989) Tobacco genes expressed during in vitro floral initiation and their expression during normal plant development, Plant Cell 1, 25–35.

    PubMed  CAS  Google Scholar 

  • Meimeth, T., Tran Thanh van, K., Marcotte, J.L., Trinh, H.T. and Clarke, A.E. (1982) Distribution of lectin in tissues, derived callus and roots of Psophocarpus tetragonolobus (Winged Bean), Plant Physiol 70, 579–584.

    Article  PubMed  CAS  Google Scholar 

  • Mo, L.H., Egertsdotter, U. and Arnold, S.V. (1996) Secretion of specific extracellular proteins by somatic embryos of Picea abies depends on embryo morphology, Ann Bot 77, 143–152.

    Article  CAS  Google Scholar 

  • Mohnen, D., Eberhard, S., Marfa, V, Doubrava, N., Toubart, P., Gollin, D.J., Gruber, T.A., Nun, W., Albersheim, P. and Darwill, A. (1990) The control of root, vegetative shoot. and flower morphogenesis in tobacco thin cell layer explants (TCLs), Development 108, 191–201.

    PubMed  CAS  Google Scholar 

  • Monteuuis, O. (1991) Rejuvenation of a 100-year Sequoiadendron giganteum through in vitro men stem culture. I. Organogenic and morphological arguments, Physiol. Plant 81, 111–115.

    Article  Google Scholar 

  • Mulin, M. and Tran Thanh Van, K. (1989) Obtention of in vitro flowers from thin epidermal cell layers of Petunia hybrida (Hors.), Plant Sei 62, 113–121.

    Article  Google Scholar 

  • Nassogne, G, Havelange, A., Marcotte, J.C., Bernier, G. and Tran Thanh Van, K. (1985) Changes in mitotic activities and cell shapes dunng flower and root neoformation in thin cell layer from tobacco influorescence, Arch. Int. Phys. Bioch 93, 14–16.

    Google Scholar 

  • Neale, A.D., Wahleithner, J.A., Lund, M., Bonnet, H.T., Kelly, A., Meeks-Wagner, R., Peacock, W.J. and Dennis, E.S. (1990) Chitinase, f3–1,3-glucanase, osmotin and extensin are expressed in tobacco explants during flower formation, Plant Cell 2, 673–684.

    PubMed  CAS  Google Scholar 

  • Nhut, D.T. (1998) Micropropagation of lily (Lilium longiflorum) via in vitro stem node and pseudo-bulblet culture, Plant Cell Rep 117, 913–916.

    Article  Google Scholar 

  • Nhut, D.T., Bui, V.L., Tanaka, M. and Tran Thanh Van, K. (2001a) Shoot induction and plant regeneration from receptacle tissue of Lilium longiflorum, Sci Hort 87, 131–138.

    Article  Google Scholar 

  • Nhut, D.T., Bui, V.L., Fukar, S., Tanaka, M. and Tran Thanh Van, K. (2001b) Effects of activated charcoal, explant size, explant position and sucrose concentration on plant and shoot regeneration of Lilium longiflorum via young stem culture, Plant Growth Reg 33, 59–65.

    Article  Google Scholar 

  • Nhut, D.T., Bui. V.L. and Tran Thanh Van, K. (2001e) Manipulation of the morphogenetic pathways of Lilium longiflorum transverse thin cell layer explants by auxin and cytokinin, In Vitro Cell Dev. Bio 37, 44–49.

    Google Scholar 

  • Nhut, D.T., Bui, V.L. and Tran Thanh Van, K. (2000) Somatic embryogenesis and direct shoot regeneration of rice (Oryza sativa L.) using thin cell layer culture of apical meristematic tissue,.1 Plant Physiol 157, 559–565.

    CAS  Google Scholar 

  • Okole, B.N. and Schulz, F.A. (1996) Micro-sections of banana and plantains (Musa spp) morphogenesis and regeneration of callus and shoot buds, Plant Sci 116, 185–195.

    Article  CAS  Google Scholar 

  • Pelissier, B., Bouchefra, O., Pepin, R. and Freyssinet, G. (1990) Production of isolated somatic embryos from sunflower thin cell layers, Plant Cell Rep 9, 47–50.

    Article  Google Scholar 

  • Pua, E.C., Trinh, T.H. and Chua, N.H. (1989) High frequency plant regeneration from stem explants of Brassica alboglabra Bailey in vitro, Plant Cell Tiss. Org . Cult 17, 143–152.

    Article  Google Scholar 

  • Pua, E.C., Mehra-Palta, A., Nagy, F. and Chua, N.H. (1987) Transgenic plants of Brassica napes L. Bio/Technology 5, 815–817.

    Article  Google Scholar 

  • Richard, L., Ano, M., Hoebeke, J., Meek-Wagner, D.R. and Tran Thanh Van, K. (1991) Immunological evidence of thaumatin like proteins during in vitro and in vivo tobacco floral differentiation, Plant Physiol 98, 337–342.

    Article  Google Scholar 

  • Schricke, H., Joulain, D. and Tran Thanh Van, K. (1988) Iris pallida: une source d’irons en culture in vitro, in Proc, Symp. European IAPTC. Bioproduction de metabolites par culture de cellules vegetales. Paris, France.

    Google Scholar 

  • Spiro, M.D., Ridley, B.L., Eberhard, S., Kales, K.A., Mathieu, Y., 0-Neill, M.A., Mohnen, D., Guem, J., Darvill, A. and Albersheim, P. (1998) Biological activity of reducing-end-derivatized oligogalacturonides in tobacco tissue culture, Plant Physiol 116, 1289–1298.

    Article  PubMed  CAS  Google Scholar 

  • Sugimura, Y. and Salvana, M.J. (1989) Induction and growth of callus derived from rachilla explants of young inflorescences of coconut palm, Can. J. Bot 67, 272–274.

    Article  Google Scholar 

  • Thorpe, T., Tran Thanh Van, K. and Gaspar, T. (1978) Isoperoxidases in epidermal layers of tobacco and changes during organ formation in vitro, Physiol. Plant 44, 388–394.

    Article  CAS  Google Scholar 

  • Tiburcio, A.F., Kaur-Sawhney, R. and Galston, A. (1988) Polyamine biosynthesis during vegetative and floral bud differentiation in thin layer tobacco tissue culture, Plant Cell Physiol 29, 1241–1249.

    CAS  Google Scholar 

  • Tiburcio, A.F., Gendy, C.A. and Tran Thanh Van, K. (1989) Morphogenesis in tobacco subepidermalcells: putrescine as marker of root differentiation, Plant Cell Tiss Org Cult 19, 43–54.

    Article  CAS  Google Scholar 

  • Tran Thanh Van, M. (1973a) In vitro control of de novo flower, bud, root and callus differentiation from excised epidermal tissues, Nature 246, 44–45.

    Google Scholar 

  • Tran Thanh Van, M. (1973b) Direct flower neoformation from superficial tissue of small explant of Nicotiana tabacum, Planta 115, 87–92.

    Article  Google Scholar 

  • Tran Thanh Van, K., Thi Dien, N., and Chlyah, A. (1974) Regulation of organogenesis in small explants of superficial tissue of Nicotiana tabacum L, Planta 119, 145–149.

    Google Scholar 

  • Tran Thanh Van, M. (1974b) Methods of acceleration of growth and flowering in a few species of orchids, Am. Orchid. Soc. Bull 43, 699–704.

    Google Scholar 

  • Tran Thanh Van, K. and Trinh, T.H. (1978) Plant propagation: non identical and identical copies, In: Hughes, K.W., Henke, R. and Constantin, M. (Eds.) Propagation of higher plants through tissue culture, Proc. Int. Sympo. Held at Univ. Teness, Knoxville, pp. 135–158.

    Google Scholar 

  • Tran Thanh Van, M. (1981) Control of morphogenesis, Ann. Rev. Plant Physiol 32, 291–311.

    Article  Google Scholar 

  • Tran Thanh Van, K., Toubart, P., and Cousson, A., Darvill, A.G, Gollin, D.J., Chelf, P. and Albersheim, P. (1985a) Manipulation of the morphogenetic pathways of tobacco explants by oligosaccharins, Nature 314, 615–617.

    Article  Google Scholar 

  • Tran Thanh Van, K., Yilmaz, A. and Trinh, H.T. (1985b) How to programme in vitro different morphogenetic expression in some conifers, In: J.M. Bonga and D.J. Durzan (Eds.) Cell and Tissue Culture in Forestry, Martinus Nijhoff Publishers, Dordrech.

    Google Scholar 

  • Tran Thanh Van, K., Lie-Schricke, H., Marcotte, J.L. and Trinh, T.H. (1986) Winged bean (Psophocarpus tetragonolobus L. DC), In: Bajaj, Y.P.S. (Ed.) Biotechnology in agriculture and forestry. Crop I Vol 2, Springer-Verlag, Berlin Heidelberg, New York. pp. 556–567.

    Google Scholar 

  • Tran Thanh Van, K., and Mutaftschiev, S. (1990) Signals influencing cell elongation, cell enlargment, cell division and morphogenesis, In: Nijkam, FI.J.J., Van Der Plas, LII.W. and Aartij, J. (Eds.) Progress in Plant Cellular and Molecular Biology, Kluwer Academic, pp. 514–519.

    Google Scholar 

  • Tran Thanh Van, K. (1991) Molecular aspects of flowering. In: Harding, J., Sing, F. and Mol, J.N.M. (Eds.) Genetic and breeding of omamental plants, Springer-Verlag, Berlin, Heidelberg, Newyork, pp. 367–385.

    Google Scholar 

  • Tran Thanh Van, K. and Gendy, C.A. (1993) Relation between come cytological, biochemical, molecular markers and plant morphogenesis, In: Roubelakis-Angelakis, K.A. and Tran Thanh Van, K. (Eds.) Plant morphogenesis and molecular approaches, Plenum press, pp. 39–45.

    Google Scholar 

  • Tran Thanh Van, K. and Gendy, C. (1996) Thin cell layer (TCL) method to programme morphogenetic patterns, Plant Tissue Culture Manual 114, 1–25.

    Google Scholar 

  • Tran Thanh Van, K. and Bui, V.L. (2000) Current status of Thin Cell layer method for the induction of organogenesis or somatic embryogenesis. In S.M. Jain, P.K. Gupta and R.J. Newton (Eds.). Somatic embryogenesis in woody plants 6, 51–92.

    Google Scholar 

  • Trinh, H.T., Lie-Schricke, H. and Tran Thanh Van, K. (1981) Formation directe de bourgeon a partir des fragments et des couches cellulaires minces de differents organs chez le Psophocaip.ts tetragonolobus (L.), Z P lanzenphysiol 102, 127–139.

    Google Scholar 

  • Trinh, H.T., Mante, S., Pua, E-C. and Chua, N-H (1987) Rapid production of transgenic flowering shoots and FI progeny from Nicotiana plumbaginifolia epidermal peels, Biotechnology 5, 1081–1084.

    Article  Google Scholar 

  • Villalobo, Y.M., Yeung, E.C. and Thorpe, T.A. (1985) Origin of adventitious shoot formation in excised Rechuta pine cotyledons culture in vitro, Can. J Bot 63, 2172–2176.

    Article  Google Scholar 

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Authors and Affiliations

  1. Institut de Biotechnologie des Plantes, UMR 8168-Université de Paris-Sud, 91405, Orsay Cedex, France

    K. Tran Thanh Van

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  1. K. Tran Thanh Van
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Editors and Affiliations

  1. Institute of Biology in Dalat, Dalat, Lam Dong, Vietnam

    Duong Tan Nhut

  2. University of Ho Chi Minh City, Ho Chi Mih City, Vietnam

    B. Van Le

  3. Institut de Biotechnologie des Plantes, Paris, France

    K. Tran Thanh Van

  4. University of Calgary, Edmonton, Canada

    T. Thorpe

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Van, K.T.T. (2003). Thin Cell Layer Concept. In: Nhut, D.T., Van Le, B., Tran Thanh Van, K., Thorpe, T. (eds) Thin Cell Layer Culture System: Regeneration and Transformation Applications. Springer, Dordrecht. https://doi.org/10.1007/978-94-017-3522-3_1

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  • DOI: https://doi.org/10.1007/978-94-017-3522-3_1

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