Abstract
The development of primary liver cell culture techniques has a long standing history that has contributed significantly to our current knowledge about the relevance of individual aspects of the hepatic microenvironment. These aspects include the composition of the extracellular matrix, matrix geometry, the presence of various cocultured cell types and oxygen supply, to name just a few. In this chapter we have tried to explain the rationale for a rather holistic and integrative approach to the problem of culturing primary liver cells. Our group has developed the concept of reconstructing the three-dimensional (3-D) plate configuration of the hepatic microenvironment to include the adjacent portions of the hepatic sinusoid [1].
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References
Bader A, Knop E, Kern A, Boker K, Frühauf N et al. 3-D coculture of hepatic sinusoidal cells with primary hepatocytes-Design of an organotypical model. Exp Cell Res 1996; 226: 223–233.
Knop E, Bader A, Böker K, Pichlmayr R and Sewing KF. Normal ultrastructure of primary hepatocytes depends on bipolar attachment to extracellular matrix. Anatomical Record 1995; 242: 337–349.
Bader A, Knop E, Böker KHW, Crome O, Frühauf N et al. Tacrolimus (FK 506) metabolism in primary rat hepatocytes depends on extracellular matrix geometry. NS Arch Pharm 1996; 353: 461–473.
Schuetz EG, Li D, Omiecinski CJ, Muller EU, Kleinman HK et al. Regulation of gene expression in adult rat hepatocytes cultured on a basement membrane matrix. J Cell Physiol 1988; 134: 309–323.
Dunn JC, Yarmush ML, Koebe HG and Tompkins RG. Hepatocyte function and extracellular matrix geometry: long-term culture in a sandwich configuration. Faseb J 1989; 3: 174–177.
Bouwens L, De Bleser P, Vaderkerken K, Geerts B and Wisse E. Liver cell heterogeneity: functions of non-parenchymal cells. Enzyme 1992; 46: 155–168.
Bouwens L and Wisse E. Recent insights into hepatic “pit cells” or natural killer cells. Reg Immunol 1988; 2: 137–141.
Altin JC and Bygrave FL. Non-parenchymal cells as mediators of physiological responses in the liver. Mol Cell Biochem 1988; 83: 3–14.
Casteleijn E, Kuiper J, Van RH, Koster JF and Van BT. Conditioned media of Kupffer and endothelial liver cells influence protein phosphorylation in parenchymal liver cells. Involvement of prostaglandins. Biochem J 1988; 252: 601–605.
Billiar TR, Curran RD, Williams DL and Kispert PH. Liver non-parenchymal cells are stimulated to provide interleukin 6 for induction of the hepatic acute-phase response in endotoxemia but not in remote localized inflammation. Arch Surg 1992; 127: 31–36.
Villafuerte BC, Koop BL, Pao CI, Birdsong GG and Phillips LS. Coculture of primary rat hepatocytes and non-parenchymal cells permits expression of insulin-like growth factor binding protein-3 in vitro. Endocrinology 1994; 134: 2044–2050.
Shimaoka S, Nakamura T and Ichihara A. Stimulation of growth of primary cultured adult rat hepatocytes without growth factors by coculture with non-parenchymal liver cells. Exp Cell Res 1987; 172: 228–242.
Holstege A, Leser HG, Pausch J and Gerok W. Uridine catabolism in Kupffer cells, endothelial cells, and hepatocytes. Eur J Biochem 1985; 149: 169–173.
Oesch F, Lafranconi M and Glatt HR. Role of parenchymal versus non-parenchymal cells in the control of biologically reactive intermediates. Adv Exp Med Biol 1986; 197: 53–61.
Steinberg P, Schramm H, Schladt L, Robertson LW, Thomas H and Oesch F. The distribution, induction and isoenzyme profile of glutathione S-transferase and glutathione peroxidase in isolated rat liver parenchymal, Kupffer and endothelial cells. Biochem J 1989; 264: 737–744.
Bader A, Rinkes IHB, Closs IE, Ryan CM, Toner M et al. A stable long-term hepatocyte culture system for studies of physiologic processes: Cytokine stimulation of the acute phase response in rat and human hepatocytes. Biotechnol Prog 1992; 8: 219–225.
Reid LM and Jefferson DM. Culturing hepatocytes and other differentiated cells. Hepatology 1984; 4: 548–559.
Geerts A, Schuppan D, Lazeroms S, De Zanger R and Wisse E. Collagen type I and III occur together in hybrid fibrils in the space of Disse of normal rat liver. Hepatology 1990;: 233–241.
Reid LM, Fiorino AS, Sigal SH, Brill S and Holst PA. Extracellular matrix gradients in the space of Disse: relevance to liver biology. Hepatology 1992; 15: 1198–1203.
Reid LM, Gaitmaitan Z, Arias I, Ponce P and Rojkind M. Long-term cultures of normal rat hepatocytes on liver biomatrix. Ann N Y Acad Sci 1980; 349: 70–76.
Bissell DM, Caron JM, Babiss LE and Friedman JM. Transcriptional regulation of the albumin gene in cultured rat hepatocytes. Role of basement-membrane matrix. Mol Biol Med 1990; 7: 187–197.
Jensen MD. Diffusion in tissue culture on gas-permeable and impermeable supports. J Theor Biol 1976; 56: 443–458.
Wallach DF and Sherwood P. Diffusion in tissue cultures on gas-permeable and impermeable supports. J Theor Biol 1976; 56: 443–458.
Holzer C and Maier P. Maintenance of periportal and pericentral oxygen tensions in primary rat hepatocyte cultures: Influence on cellular DNA and protein content monitored by flow cytometry. J Cell Physiol 1987; 133: 297–304.
Bader A, Frühauf N, Tiedge M, Drinkgern M, DeBartolo L et al. Enhanced oxygen delivery reverses anaerobic metabolic states in prolonged sandwich rat hepatocyte culture. Exp Cell Research 1999; 246: 221–232.
Bader A, Frühauf N, Haverich A and Borlak J. Development of a small scale bioreactor for drug metabolism studies. Xenobiotica 1998; 28: 815–825.
Bader A, De Bartolo L and Haverich A. Initial evaluation of the performance of a scaled up flat membrane bioreactor (FMB) with pig liver cells. In: Muraca S, Ed. Bioartificial Liver: The critical issues. Padova: CIC Edizioni Internazionali, 1997: 36–41.
Elsdale T and Bard J. Collagen substrata for studies on cell behavior. J Cell Biol 1972; 54: 626–637.
Hall HG, Farson DA and Bissell MJ. Lumen formation by epithelial cell lines in response to collagen overlay: a morphogenetic model in culture. Proc Natl Acad Sci USA 1982; 79: 4672–4676.
Kern A, Bader A, Pichlmayr R and Sewing K-F. Drug metabolism in hepatocyte sandwich cultures of rats and humans. Biochem Pharmacol 1997; 54: 761–772.
Hawksworth GM. Advantages and disadvantages of using human cells for pharmacological and toxicological studies. Human Exp Toxicol 1994; 13: 568–573.
Daujat M, Pichard L, Fabre I, Pineau T, Fabre G, Bonfils C and Maurel P. Induction protocols for cytochromes P450IIIA in vivo and in primary cultures of animal and human hepatocytes. Meth Enzymol 1991; 206: 345–353.
Ged C, Rouillon JM, Pichard L, Combalbert J, Bressot N et al. The increase in urinary excretion of 6b-hydroxycortisol as a marker of human hepatic cytochrome P450IIIA induction. Br J Clin Pharmac 1989; 28: 373–387.
Zech K, Eltze M, Kilian U, Sanders KH and Kolassa N. Biotransformation of Urapidil: Metabolites in serum and urine and their biological activity in vitro and in vivo. Arch Int Pharm Ther 1984; 272: 180–196.
Berry MN and Friend DS. High-yield preparation of isolated rat liver parenchymal cells. J Cell Biol 1969; 43: 506–520.
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Bader, A., Knop, E., Haverich, A. (2000). Engineering of a miniature 3-D hepatic cell plate culture model. In: Berry, M.N., Edwards, A.M. (eds) The Hepatocyte Review. Springer, Dordrecht. https://doi.org/10.1007/978-94-017-3345-8_8
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DOI: https://doi.org/10.1007/978-94-017-3345-8_8
Publisher Name: Springer, Dordrecht
Print ISBN: 978-90-481-5402-9
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