Abstract
The original reason for attempting to prepare suspensions of intact isolated hepatocytes was to produce a preparation that would facilitate the study of hepatic metabolism. Forty years on, the great majority of metabolic pathways have been delineated and interest in metabolism has waned. Nevertheless, the manner in which flux is regulated still appears to be a fruitful area for study. In this chapter we review the results of metabolic experiments carried out in our laboratory during the thirty years that intact hepatocyte suspensions have been available for experimental work. Much of our effort during that time has been directed towards the study of mitochondrial-cytoplasmic interactions, in particular the intercompartmental transfer of reducing equivalents. We have endeavoured to elucidate the factors that determine the direction of flow, and have concluded that the relationships we observe cannot be explained solely on the basis of molecular diffusion and random collision. Instead, we put forward the hypothesis that the critical regulatory processes that determine the rate and direction of intercompartmental reducing-equivalent transfer in living cells are dependent on flow through organized systems, which is controlled by energy-dependent mechanisms.
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Phillips, J.W., Grivell, M.B., Grivell, A.R., Berry, M.N. (2000). Studies on mitochondrial-cytoplasmic interactions in isolated hepatocytes. In: Berry, M.N., Edwards, A.M. (eds) The Hepatocyte Review. Springer, Dordrecht. https://doi.org/10.1007/978-94-017-3345-8_21
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DOI: https://doi.org/10.1007/978-94-017-3345-8_21
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