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Vibrio cholerae in recreational beach waters and tributaries of Southern California

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The Ecology and Etiology of Newly Emerging Marine Diseases

Part of the book series: Developments in Hydrobiology ((DIHY,volume 159))

Abstract

Vibrio cholerae is the causative agent of the severe dehydrating diarrheal disease cholera. This bacterium has been detected in many estuaries around the world and the United States. In this study we examine the abundance and distribution of V cholerae in recreational beach waters and tributaries of Southern California. Water samples were taken from 11 beach locations adjacent to freshwater runoff sources between February 8th and March Ist, 1999. Water samples were also taken from rivers, creeks and coastal wetlands along the Southern California coast between May 19th and June 28th, 1999. In addition to the detection of V cholerae, environmental parameters including temperature, salinity, coliphage counts, viable heterotrophic plate counts and total bacterial direct counts were also determined to understand the relationships between the presence of V. cholerae and environmental conditions. A direct colony hybridization method using an oligonucleotide probe specific for the 16S-23S intergenic spacer region of V. cholerae,detected V. cholerae in 3 of the 11 beach samples with the highest concentration (60.9 per liter) at the mouth of Malibu Lagoon. V. cholerae and coliphage were not correlated for beach samples, indicating that the presence of V cholerae is independent of sewage pollution. V cholerae were detected in all samples taken from rivers, creeks and wetlands of coastal Southern California where salinities were between l to 34 parts per thousand (ppt), but was not found at a freshwater sampling site in upper San Juan Creek. The highest density of V cholerae was found in San Diego Creek with a concentration of 4.25 x 105 CFU/L. The geographical distribution of V. cholerae was inversely correlated with salinity. High concentrations of V cholerae were more frequently detected in waters with lower (but above 0) salinity. The results of this study provide insight into the ecology of this aquatic species and are potentially important to the understanding of the epidemiology of cholera on a global scale.

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Authors and Affiliations

  1. Environmental Analysis and Design, University of California, 1367 Social Ecology II, Irvine, CA, 92697-7070, USA

    Sunny C. Jiang

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  1. Sunny C. Jiang
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Editors and Affiliations

  1. Institute of Ecology, University of Georgia, Athens, USA

    James W. Porter

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© 2001 Springer Science+Business Media Dordrecht

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Jiang, S.C. (2001). Vibrio cholerae in recreational beach waters and tributaries of Southern California. In: Porter, J.W. (eds) The Ecology and Etiology of Newly Emerging Marine Diseases. Developments in Hydrobiology, vol 159. Springer, Dordrecht. https://doi.org/10.1007/978-94-017-3284-0_14

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  • DOI: https://doi.org/10.1007/978-94-017-3284-0_14

  • Publisher Name: Springer, Dordrecht

  • Print ISBN: 978-90-481-5930-7

  • Online ISBN: 978-94-017-3284-0

  • eBook Packages: Springer Book Archive

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