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Techniques of AM Fungus Inoculum Production

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Book cover Techniques in Mycorrhizal Studies

Abstract

The broad applications of AM fungi has been limited by the difficulties of obtaining large quantities of pure inoculum of the obligate fungal symbiont. AM fungi have a variety of propagules (spores, hyphal fragments and hyphae within senesced and living roots). Resting spores extracted from soil are often used as inoculum. Aeroponic culture of AM fungi is a technique that enables efficient production of inoculum and soil free investigations of mycorrhizae. In recent years they have been cultivated in vitro. Root organ culture, whether with transformed or non-transformed roots is expensive, labour intensive and does not give sporulation equivalent to the traditional pot cultures. However it is a valuable tool to study AM fungi. Different techniques of AM fungal inoculum production are given in this paper.

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  1. Microbiology Division, Indian Agricultural Research Institute, New Delhi, 110012, India

    Geeta Singh & K. V. B. R. Tilak

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  1. Department of Botany, University of Delhi, 237, SFS/DDA Mukerji Apts. East Mukerji Nagar, 110009, Delhi, India

    K. G. Mukerji

  2. Applied Mycology and Molecular Plant Pathology Laboratory Department of Botany, Osmania University, 500 007, Hyderabad, A.P., India

    C. Manoharachary

  3. Department of Botany, University of Delhi, Delhi, India

    B. P. Chamola

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Singh, G., Tilak, K.V.B.R. (2002). Techniques of AM Fungus Inoculum Production. In: Mukerji, K.G., Manoharachary, C., Chamola, B.P. (eds) Techniques in Mycorrhizal Studies. Springer, Dordrecht. https://doi.org/10.1007/978-94-017-3209-3_14

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