Abstract
Although the sensitivity of the plasma membrane H+-ATPase to vanadate is well known, the metabolic response of plant cells to vanadate is less well characterised in vivo and its use as an inhibitor in whole plant experiments has had mixed success. Experiments with maize (Zea mays, L.) roots and with purified plasma membrane fractions from the same tissues showed that exposure to vanadate caused: (i) a reduction in the capacity for phosphate uptake; (ii) a reduction in the extractable ATPase activity from the tissue; and (iii) a significant increase in the ATP level. The measurements on the extractable ATPase activity and the ATP level showed that the effect of vanadate developed slowly, apparently reflecting the slow accumulation of intracellular vanadate. The marked effect of vanadate on the ATP level — exposure to 500 uM vanadate for 5 h doubled the ATP content of the roots tips — indicates that there is no stringent control over the ATP level in the roots and that the plasma membrane H+ -ATPase activity is likely to have a significant role in determining the ATP level under normal conditions.
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References
Beauge L 1988 Inhibition of translocation reactions by vanadate. Methods Enzymol. 156, 251–267.
Bowman B J and Slayman C W 1979 The effects of vanadate on the plasma membrane ATPase of Neurospora crassa. J. Biol. Chem. 254, 2928–2934.
Brummell D A, Hall J L and Armstrong C F 1986 Effect of vanadate on microsomal ATPase activity, acidification and auxin stimulated growth in pea and cucumber. Ann. Bot. 57, 727–735.
Cantley L C, Cantley L G and Josephson L 1978 A characterisation of vanadate interactions with the Na+ K+ ATPase. Mechanistic and regulatory implications. J. Biol. Chem. 253, 7361–7368.
Cocucci M, Ballarin-Dente A and Marré M T 1980 Effects of orthovanadate on H+ secretion, K+ uptake, electric potential difference and membrane ATPase activities of higher plant tissues. Plant Sci. Lett. 17, 391–400.
Cowan D S C, Clarkson D T and Hall J L 1993 A comparison between the ATPase and proton pumping activities of plasma membranes isolated from the stele and cortex of Zea mays roots. J. Exp. Bot. 44, 983–989.
Gallagher S R and Leonard R T 1982 Effect of vanadate, molybdate and azide on membrane-associated ATPase and soluble phosphatase activities of corn root. Plant Physiol. 70, 1335–1340.
Grantham J J and Glynn I M 1979 Renal Na+, K+ ATPase: determinants of inhibition by vanadium. Am. J. Physiol. 236, 530–535.
Hooks M A, Clark R A, Nieman R H and Roberts J K M 1989 Compartmentation of nucleotides in corn root tips studied by 31P NMR and HPLC. Plant Physiol. 89, 963–969.
Kime M J, Loughman B C, Ratcliffe R G and Williams R J P 1982 The application of 31P nuclear magnetic resonance to higher plant tissue. I. Detection of spectra. J. Exp. Bot. 33, 656–669.
Kustin K and Toppen D L 1973 Kinetics of complexation of vanadate anions by ethylenediaminetetra acetic acid and 1,2dihydroxyanthraquinone. J. Am. Chem. Soc. 95, 3564–3568.
Lee R B and Ratcliffe R G 1983 Development of an aeration system for use in plant tissue NMR experiments. J. Exp. Bot. 34, 1213–1221.
Leonard R T and Hanson J B 1972 Increased membrane-bound ATPase activity accompanying development of enhanced solute uptake in washed corn root tissue. Plant Physiol. 49, 436–440.
Loughman B C, Ratcliffe R G and Schwabe J W R 1989 Galactose metabolism in Zea mays root tissues observed by 31P NMR spectroscopy. Plant Sci. 59, 11–23.
Loughman B C and White P J 1984 The role of minor nutrients in the control of ion movement across membranes. In Membrane Transport in Plants. Eds. A Walker and J Cram. pp 501–502. Academia, Prague.
Macara I G, Kustin K and Cantley L C 1980 Glutathione reduces cytoplasmic vanadate. Mechanism and physiological implications. Biochim. Biophys. Acta 629, 95–106.
Perlin D S and Spanswick R M 1981 Characteristics of ATPase activity associated with corn leaf plasma membranes. Plant Physiol. 68, 521–526.
Peterson G L 1977 A simplification of the protein assay method of Lowry et al. which is more generally applicable. Anal. Biochem. 83, 346–356.
Peterson G L 1978 A simplified method for analysis of inorganic phosphate in the presence of interfering substances. Anal. Biochem. 84, 164–172.
Ross H H 1976 Theory and application of Cerenkov counting. In Liquid Scintillation Science and Technology. Eds. A A Nouj aim, C Ediss and L I Wiebe. pp 79–91. Academic Press, New York.
Sze H and Hodges T K 1977 Selectivity of alkali cation influx across the plasma membrane of oat roots. Cation specificity of the plasma membrane ATPase. Plant Physiol. 59, 641–646.
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Sklenar, J., Fox, G.G., Loughman, B.C., Pannifer, A.D.B., Ratcliffe, R.G. (1995). Effects of vanadate on the ATP content, ATPase activity and phosphate absorption capacity of maize roots. In: Baluška, F., Čiamporová, M., Gašparíková, O., Barlow, P.W. (eds) Structure and Function of Roots. Developments in Plant and Soil Sciences, vol 58. Springer, Dordrecht. https://doi.org/10.1007/978-94-017-3101-0_15
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DOI: https://doi.org/10.1007/978-94-017-3101-0_15
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