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Cryopreservation of Embryogenic Cultures of Conifers and Its Application to Clonal Forestry

  • Chapter
Somatic Embryogenesis in Woody Plants

Part of the book series: Forestry Sciences ((FOSC,volume 55))

Abstract

Cryopreservation, by providing physico-chemical stability of germplasm, offers the potential for economically and reliably providing long-term storage of genetic resources (Blakesley et al. 1996). It has become a cornerstone of conifer somatic embryogenesis (Adams et al. 1994; Gupta et al. 1994, Smith et al. 1994, Handley et al. 1995), offering advantages over other vegetative propagation systems (Grossnickle et al. 1996, Park et al. 1998). Specifically, cryopreservation maintains juvenility of donor tissue, a factor that is limiting in programs using conventional rooted cuttings (Grossnickle et al. 1996); and has application to a greater array of genotypes than can be achieved with organogenesis (Smith et al. 1994, Menzies and Aimers-Halliday 1997). Within the operational forestry framework, cryopreservation enables the recovery of plants from genotypes that have been selected from long-term clonal or transgenic field trials; and provides the opportunity to establish production clone banks of elite lines for commercial clonal forestry.

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Authors and Affiliations

  1. Forest Biotechnology Centre, BC Research Inc., 3650 Wesbrook Mall, Vancouver, BC, Canada, V6S 2L2

    David R. Cyr

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Editors and Affiliations

  1. Department of Plant Production, University of Helsinki, Helsinki, Finland

    S. Mohan Jain (Book Project Leader) (Book Project Leader)

  2. Weyerhaeuser Inc., Tacoma, Washington, USA

    Pramod K. Gupta

  3. Department of Forest Science, Texas A & M University, College Station, Texas, USA

    Ronald J. Newton

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Cyr, D.R. (1999). Cryopreservation of Embryogenic Cultures of Conifers and Its Application to Clonal Forestry. In: Jain, S.M., Gupta, P.K., Newton, R.J. (eds) Somatic Embryogenesis in Woody Plants. Forestry Sciences, vol 55. Springer, Dordrecht. https://doi.org/10.1007/978-94-017-3032-7_10

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  • DOI: https://doi.org/10.1007/978-94-017-3032-7_10

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