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Cryopreservaton of Embryogenic Calli of Hevea brasiliensis

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Somatic Embryogenesis in Woody Plants

Part of the book series: Forestry Sciences ((FOSC,volume 67))

Abstract

Various problems are encountered in the establishment and maintenance of embryogenic cultures. The production of embryogenic material is often a difficult and lengthy process, as observed notably with oilpalm and coconut (Duval et al. 1995; Verdeil and Buffard-Morel 1995). The progressive decrease of embryogenic potential over time is a phenomenon which is commonly observed with embryogenic cultures (Withers 1985). Moreover, risks of somaclonal variation increase in line with the extension of the culture period (Reisch 1984). Finally, it is difficult and costly to maintain large numbers of proliferating cultures in large-scale production laboratories. Tools which would allow to overcome these difficulties are urgently needed to enhance the applicability of somatic embryogenesis processes for the mass propagation of elite material.

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Authors and Affiliations

  1. International Plant Genetic Resources Institute, IPGRI, Via delle Sette Chiese 142, 00145, Rome, Italy

    Florent Engelmann

  2. (Centro Agronomico Tropical de Investigacíon y Enseñenza/Centre de coopération internationale en recherche agronomique pour le développement-Département cultures pérennes), CATIE/CIRAD-CP, Apartado 11, 7170, Turrialba, Costa Rica

    Hervé Etienne

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  1. Florent Engelmann
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  2. Hervé Etienne
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Editors and Affiliations

  1. Plant Breeding and Genetics Section, FAO/IAEA Division, International Atomic Energy Agency, Vienna, Austria

    S. Mohan Jain

  2. Weyerhaeuser Inc., Tacoma, Washington, USA

    Pramod K. Gupta

  3. Department of Biology, East Carolina University Greenville, North Carolina, USA

    Ronald J. Newton

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Engelmann, F., Etienne, H. (2000). Cryopreservaton of Embryogenic Calli of Hevea brasiliensis . In: Jain, S.M., Gupta, P.K., Newton, R.J. (eds) Somatic Embryogenesis in Woody Plants. Forestry Sciences, vol 67. Springer, Dordrecht. https://doi.org/10.1007/978-94-017-3030-3_27

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  • DOI: https://doi.org/10.1007/978-94-017-3030-3_27

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