Hepatotoxic cyanobacteria in the shallow lake Müggelsee

  • Martin Welker
  • Sigrid Hoeg
  • Christian Steinberg
Part of the Developments in Hydrobiology book series (DIHY, volume 143)


Since 1996 we have studied the occurrence of cyanobacterial hepatotoxins, microcystins (Mcyst), in the shallow lake Müggelsee. Intracellular (cell-bound) Mcyst’s were detectable from late summer to autumn in 2 years, and maximum concentrations of about 3 μg/1 of total cell-bound Mcyst were measured. The concentration of Mcyst’s was correlated to the biovolume of Microcystis sp. (R 2=0.560 in 1996 and R 2=0.483 in 1997; P <0.001). Between years, the ratio of Mcyst’ss per biovolume of Microcystis differed significantly (P <0,001; 0.913 μg/mm3 in 1996 and 0.171 μg/mm3 in 1997). The ratio of the detected Mcyst variants, Mcyst-LR and Mcyst-RR, was constant in all samples with Mcyst-LR:Mcyst-RR ≈ 1:2.9. One out of 22 strains of Microcystis sp. we isolated in 1997 contained Mcyst-LR and Mcyst-RR at a ratio of Mcyst-LR:Mcyst-RR=1:1.6. The Mcyst’ss found in lake samples were likely to have been produced by a number of a few strains that were present in the lake for extended periods of time in both years.

Key words

cyanobacteria Microcystis sp. Aphanizomenon sp. microcystin 


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  1. Carmichael W. W., 1992. Cyanobacteria secondary metabolites — the cyanotoxins. J. Appl. Bact. 72: 445–459.CrossRefGoogle Scholar
  2. Chorus, I., 1993. Algal metabolites and water quality: toxins, allergenes, and taste-and-odor-substances. Mem. ital. Idrobiol. 52: 257–280.Google Scholar
  3. Driescher, E., H. Behrendt, G. Schellenberger & R. Stellmacher, 1993. Lake Müggelsee and its environment — natural conditions and anthropogenic impacts. Int. Revue ges. Hydrobiol. 78: 237343.Google Scholar
  4. Erhard, M., H. von Döhren & P. Jungblut, 1997. Rapid typing and elucidation of new secondary metabolites of intact cyanobacteria using MALDI-TOF mass spectroscopy. Nature Biotechnol. 15: 906–909.CrossRefGoogle Scholar
  5. Fastner, J., M. Erhard, W. W. Carmichael, F. Sun, K. L. Rinehart, H. Rönicke & I. Chorus, 1999. Characterization of microcystins in natural blooms and strains of the genera Microcystis and Planktothrix from German freshwaters. Arch. Hydrobiol. 145: 147–163.Google Scholar
  6. Harada, K., 1996. Chemistry and detection of microcystins. In Watanabe, M. F., K.-I. Harada, W. W. Carmichael & H. Fujiki (eds), Toxic Microcystis. CRC, Boca Raton, U.S.A: 103–148.Google Scholar
  7. Köhler, J., H. Behrendt & S. Hoeg, Phytoplankton development in the shallow lake Müggelsee and the inflowing Spree during the last two decades (in press).Google Scholar
  8. Komarek, J. & K. Anagnostidis, 1999. Cyanoprokaryota-1. Teil: Chroococcales. In Ettl, H., G. Gärtner, H. Heynig, D. Mollenhauer (eds), Süßwasserflora von Mitteleuropa. Gustav Fischer, Jena.Google Scholar
  9. Lawton, L. A., C. Edwards & G. A. Codd,. 1994. Extraction and High-Performance Liquid Chromatography method for the determination of microcystins in raw and treated waters. Analyst 119: 1525–1530.PubMedCrossRefGoogle Scholar
  10. Orr, P. T. & G. J. Jones, 1998. Relationship between microcystin production and cell division rates in nitrogen limited Microcystis aeruginosa cultures. Limnol. Oceanogr. 43: 1604–1614.CrossRefGoogle Scholar
  11. Park, H.-D., C. Iwami, M. F. Watanabe, K.-I. Harada, T. Okino & H. Hayashi, 1998. Temporal variabilities of the concentration of intra-and extracellular microcystin and toxic Microcystis species in a hypertrophic lake, Lake Suwa, Japan (1991–1994). Envir. Toxicol. Water Qual. 13: 61–72.CrossRefGoogle Scholar
  12. Rapala, J., K. Sivonen, C. Lyra & S.I. Niemelä, 1997. Variation of microcystins, cyanobacterial hepatotoxins, in Anabaena spp. as a function of growth stimuli. Apl. envir. Microbiol. 63: 22062212.Google Scholar
  13. Ueno, Y., S. Nagata, T. Tsutsumi, A. Hasewaga, F. Yoshida, M. Suttajit, D. Mebs, M. Putsch & V. M. Vasconcelos, 1996. Survey of microcystins in environmental water by a highly sensitive immunoassay based on monoclonal antibody. Natural Toxins 4: 271–276.PubMedCrossRefGoogle Scholar
  14. Watanabe, M., 1996. Isolation, cultivation, and classification of bloom-forming Microcystis in Japan. In Watanabe, M. F., K.-I. Harada, W. W. Carmichael & H. Fujiki (eds), Toxic Microcystis. 2, CRC, Boca Raton, U.S.A: 13–34.Google Scholar
  15. Watanabe, M. F., 1996. Production of microcystins. In Watanabe, M. F., K.-I. Harada, W. W. Carmichael & H. Fujiki (eds), Toxic Microcystis. CRC, Boca Raton, U.S.A: 35–56.Google Scholar
  16. Zehnder, A. & P. R. Gorham, 1960. Factors influencing the growth of Microcystis aeruginosa KUTZ. emend. ELENKIN. Can J. Microbiol. 6: 645–660.PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media Dordrecht 1999

Authors and Affiliations

  • Martin Welker
    • 1
  • Sigrid Hoeg
    • 1
  • Christian Steinberg
    • 1
  1. 1.Institut für Gewässerökologie und Binnenfischerei (IGB)BerlinGermany

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