Abstract
X-ray micro-tomography is a well-established technique for non-invasive imaging and evaluation of heterogeneous materials. An inexpensive X-ray micro-tomography system has been designed and built for the specific purposes of examining root growth and root/soil interactions. The system uses a silver target X-ray source with a focal spot diameter of 80 gm, an X-ray image intensifier with a sampling aperture of about 100 μm,and a sample with a diameter of 25 mm. Pre-germinated wheat and rape seeds were grown for up to 8–10 days in plastic containers in a sandy loam soil sieved to < 250 μm, and imaged with the X-ray system at regular intervals. The quality of 3 D image obtained was good allowing the development and growth of both root axes and some first-order laterals to be observed. The satisfactory discrimination between soil and roots enabled measurements of root diameter (wheat values were 0.48–1.22 mm) in individual tomographic slices and, by tracking from slice to slice, root lengths were also measured. The measurements obtained were generally within 10% of those obtained from destructive samples measured manually and with a flat-bed scanner. Further developments of the system will allow more detailed examination of the root:soil interface.
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References
Adderley W P, Simpson I A and MacLeod G W 2001 Testing high-resolution X-ray computed tomography for the micromorphological analyses of archaeological soils and sediments. Archaeol. Prospect. 8, 107–112.
Asseng S, Aylmore L A G, MacFall J S, Hopmans J W and Gregory P J 2000 Computer-assisted tomography and magnetic resonance imaging. In Techniques for Studying Roots. Eds. A L Smit, A G Bengough, C Engels, M van Noordwijk, S Pellerin and S C van de Geijn. pp. 343–363. Springer, Berlin.
Aylmore L A G 1993 Use of computer-assisted tomography in studying water movement around plant roots. Adv. Agron. 49, 1–54.
Barrett H H and Swindell W 1981 Radiological Imaging: The Theory of Image Formation, Detection and Processing. pp 348–349. Academic Press, London.
Bragg P L, Govi G and Cannell R Q 1983 A comparison of methods, including angled and vertical minirhizotrons, for studying root growth and distribution in a spring oat crop. Plant Soil 73, 435440.
Brown J M, Johnson G A and Kramer P J 1986 In vitro magnetic resonance microscopy of changing water content in Pelargonium hortorum roots. Plant Physiol. 82, 1158–1160.
Gregory P J 1979 A periscope method for observing root growth and distribution in field soil. J. Exp. Bot. 30, 205–214.
Hainsworth J M and Aylmore L A G 1983 The use of computer-assisted tomography to determine spatial distribution of soil water content. Aust. J. Soil Res. 21, 435–443.
Hamza M A and Aylmore L A G 1992 Soil solute concentration and water uptake by single lupin and radish plant roots. I. Water extraction and solute accumulation. Plant Soil 145, 187–196.
Hamza M A, Anderson S H and Aylmore L A G 2001 Studies of soil water drawdowns by single radish roots at decreasing soil water content using computer-assisted tomography. Aust. J. Soil Res. 39, 1387–1396.
Heeraman D A and Juma N G 1993. A comparison of minirhizotron, core and monolith methods for quantifying barley (Hordeum vulgare L.) and fababean (Vicia faba L.) root distribution. Plant Soil 148, 29–41.
Heeraman D A, Hopmans J W and Clausnitzer V 1997 Three dimensional imaging of plant roots in situ with X-ray computed tomography. Plant Soil 189, 167–179.
Hopmans J W, Vogel T and Koblik P D 1992 X-ray tomography of soil water distribution in one-step outflow experiments. Soil Sci. Soc. Am. J. 56, 355–362.
Hounsfield G N 1972 A method of and apparatus for examination of a body by radiation such as X- or gamma radiation. British Pat. No. 1283915, London, UK.
Jarvis R A 1968 Soils of the Reading District. Memoir of the Soil Survey of Great Britain England and Wales. Harpenden, Herts, UK.
Jenneson P M, Morton E J and Gilboy W B 2002 An X-ray micro-tomography system optimised for the low-dose study of living organisms. Applied Rad. Isotopes (in press).
Ketcham R A and Carlson W D 2001 Acquisition, optimization and interpretation of X-ray computed tomographic imagery: applications to the geosciences. Comp. Geosci. 27, 381–400.
Kirchoff G and Pendar CE 1995 Delta-T Scanner — User Manual v2. 0. Ed. N Webb. Delta-T Devices Ltd., Cambridge, U.K.
Livesley S J, Stacey C L, Gregory P J and Buresh R J 1999. Sieve size effects on root length and biomass measurements of maize (Zea mays) and Grevillea robusta. Plant Soil 207, 183–193.
MacFall J S and Johnson G A 1994 Use of magnetic resonance imaging in the study of plants and soils. In Tomography of SoilWater-Root Processes. Eds. S H Anderson and J W Hopmans. pp. 99–113. SSSA Special Publication no. 36. SSSA, Madison.
Macedo A, Crestana S and Vaz C M P 1998 X-ray microtomography to investigate thin layers of soil clod. Soil Till. Res. 49, 249–253.
Pierret A, Capowiez Y, Moran C J and Kretzschmar A 1999 X-ray computed tomography to quantify tree rooting spatial distributions. Geoderma 90, 307–326.
Smit A L, George E and Groenwold J 2000 Root observations and measurements at (transparent) interfaces with soil. In Techniques for Studying Roots. Eds. A L Smit, A G Bengough, C Engels, M van Noordwijk, S Pellerin and S C van de Geijn. pp. 236–271. Springer, Berlin.
Taylor H M 1987 Minirhizotron Observation Tubes: Methods and Applications for Measuring Rhizosphere Dynamics. ASA Special Publ. 50. ASA, CSSA and SSA, Madison, WI.
Young I M, Crawford J W and Rappoldt C 2001 New methods and models for characterising structural heterogeneity of soil. Soil Till. Res. 61, 33–45.
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Gregory, P.J., Hutchison, D.J., Read, D.B., Jenneson, P.M., Gilboy, W.B., Morton, E.J. (2003). Non-invasive imaging of roots with high resolution X-ray micro-tomography. In: Abe, J. (eds) Roots: The Dynamic Interface between Plants and the Earth. Developments in Plant and Soil Sciences, vol 101. Springer, Dordrecht. https://doi.org/10.1007/978-94-017-2923-9_34
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DOI: https://doi.org/10.1007/978-94-017-2923-9_34
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