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Rhizoctonia Species: Taxonomy, Molecular Biology, Ecology, Pathology and Disease Control pp 81–86Cite as

Molecular Analysis of Ribosomal RNA Genes in Rhizoctonia Fungi

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Abstract

The DNA sequences that encode ribosomal RNAs have been useful for understanding phylogenetic and taxonomic relationships and patterns of genetic variation in fungi (Bruns et al.,1992). In fungi, ribosomal genes (rDNA) are located within mitochondria or nuclei and contain numerous regions of high sequence conservation and variability over their entire length (White et al., 1990). The nuclear rDNA genes of fungi are arranged as a series of tandemly repeated units that include genes for the 18S (small), 5.8S and 28S (large) rRNA subunits. Vilgalys and Gonzalez (1990a) determined that Thanatephorus praticola (anamorph = Rhizoctonia solani AG 4) had approximately 59 copies (repeated units) per haploid genome. The 28S and 18S rRNA subunits are separated by an intergenic spacer (IGS) region that usually contains a 5S rRNA subunit and the 5.8S subunit is flanked by two internal transcribed spacer (ITS 1 and ITS2) regions. In contrast to nuclear rDNA genes, mitochondrial (mt) rDNA genes have a lower guanine and cytosine content (25–40%), possess more methylated nucleotides and lack a 5.8S subunit (White et al.,1990). Except for analysis of the small mt rRNA subunit (Liu and Sinclair, 1992), mt rDNA genes have not been critically examined in Rhizoctonia fungi.

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Author information

Authors and Affiliations

  1. Department of Plant Pathology, North Carolina State University, Vernon G. James Research and Extension Center, 207 Research Station Road, Plymouth, NC, 27962, USA

    Marc A. Cubeta

  2. Department of Botany, Duke University, Durham, NC, 27708, USA

    Rytas Vilgalys

  3. Instituto de Ecologia, A.C., 91000, Xalapa, Vera Cruz, Mexico

    Dolores Gonzalez

Authors
  1. Marc A. Cubeta
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  2. Rytas Vilgalys
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  3. Dolores Gonzalez
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Editor information

Editors and Affiliations

  1. Tel Aviv University, Ramat Aviv, Tel Aviv, Israel

    Baruch Sneh

  2. McDonald Campus, McGill University, Quebec, Canada

    Suha Jabaji-Hare

  3. CSIRO, Division of Soils, Adelaide, South Australia, Australia

    Stephen Neate

  4. Research Institute for Plant Protection (IPO-DLO), Wageningen, The Netherlands

    Gerda Dijst

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© 1996 Springer Science+Business Media Dordrecht

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Cubeta, M.A., Vilgalys, R., Gonzalez, D. (1996). Molecular Analysis of Ribosomal RNA Genes in Rhizoctonia Fungi. In: Sneh, B., Jabaji-Hare, S., Neate, S., Dijst, G. (eds) Rhizoctonia Species: Taxonomy, Molecular Biology, Ecology, Pathology and Disease Control. Springer, Dordrecht. https://doi.org/10.1007/978-94-017-2901-7_7

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  • DOI: https://doi.org/10.1007/978-94-017-2901-7_7

  • Publisher Name: Springer, Dordrecht

  • Print ISBN: 978-90-481-4597-3

  • Online ISBN: 978-94-017-2901-7

  • eBook Packages: Springer Book Archive

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