Abstract
This short review summarizes recent experimental data on root plasma-membrane-bound nitrate reductase (PM-NR) mainly from tobacco roots. Although root PM-NR apparently comprises the same domains for partial reactions as leaf PM-NR and the soluble NR of roots and leaves, only this enzyme uses succinate as electron donor, in addition to NADH. Various properties and the regulation of root PM-NR by the preceding nitrate nutrition of the plants differ considerably, not only from soluble NR in the roots, but also from soluble NR and PM-NR in the leaves of the same plants.
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References
Campbell W 1996 Update on biochemistry. Nitrate reductase biochemistry comes of age. Plant Physiol. 111, 355–361.
Kunze M, Riedel J, Lange U, Hurwitz R and Tischner R 1997 Evidence for the presence of GPI-anchored PM-NR in leaves of Beta vulgaris and for PM-NR in barley leaves. Plant Physiol. Biochem. 35, 507–512.
Larsson C 1985 Plasma membranes. In Modern Methods in Plant Analysis, New Series, Vol. 1, Cell Components. Eds HF Linskens and JF Jackson. pp 85–104. Springer, Berlin.
Stöhr C 1999a Relationship of nitrate supply with growth rate, plasma membrane-bound and cytosolic nitrate reductase and tissue nitrate content in tobacco plants. Plant Cell Environ. 22, 169–177.
Stöhr C 1999b Plasma membrane-bound nitrate reductase in algae and higher plants. In Plasma Membrane Redox Systems and Their Role in Biological Stress and Disease. Eds H Asard, A Bérczi and RJ Caubergs. pp 103–119. Kluwer Academic Publishers, Dordrecht, The Netherlands.
Stöhr C, Tischner R and Ward M R 1993 Characterization of the plasma-membrane-bound nitrate reductase in Chlorella saccharophila ( Krüger) Nadson. Planta 191, 79–85.
Stöhr C, Schuler F and Tischner R 1995 Glycosyl-phosphatidylinositol-anchored proteins exist in the plasma membrane of Chlorella saccharophila ( Krüger) Nadson: Plasma-membranebound nitrate reductase as an example. Planta 196, 284–287.
Stöhr C and Ullrich W R 1997 A succinate-oxidising nitrate reductase is located at the plasma membrane of plant roots. Planta 203, 129–132.
Tischner R, Ward M R and Huffaker R C 1989 Evidence for a plasma-membrane-bound nitrate reductase involved in nitrate uptake of Chlorella sorokiniana. Planta 178, 19–24.
Ward M R, Tischner R and Huffaker R C 1988 Inhibition of nitrate transport by anti-nitrate reductase IgG fragments and the identification of plasma membrane associated nitrate reductase in roots of barley seedlings. Plant Physiol. 88, 1141–1145.
Werck-Reichhart D, Benveniste I, Teutsch H, Durst F and Gabriac B 1991 Glycerol allows low-temperature phase separation of membrane proteins solubilized in Triton X-114: Application to the purification of plant cytochromes P-450 and bs. Anal. Biochem. 197, 125–131.
Wienkoop S, Ullrich W R and Stöhr C 1999 Kinetic characteristics of succinate-dependent plasma membrane-bound nitrate reductase in tobacco roots. Physiol. Plant. 105, 609–614.
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© 2001 Springer Science+Business Media Dordrecht
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Stöhr, C., Wienkoop, S., Ullrich, W.R. (2001). Nitrate reductase in roots: Succinate- and NADH-dependent plasma membrane-bound forms. In: Gašparíková, O., Čiamporová, M., Mistrík, I., Baluška, F. (eds) Recent Advances of Plant Root Structure and Function. Developments in Plant and Soil Sciences, vol 90. Springer, Dordrecht. https://doi.org/10.1007/978-94-017-2858-4_14
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DOI: https://doi.org/10.1007/978-94-017-2858-4_14
Publisher Name: Springer, Dordrecht
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