Abstract
Phosphate is an important macronutrient of higher plants, the molecular transport mechanisms of which have not been investigated until recently. Genetical optimizing of arable crops in their phosphate acquisition efficiency is highly desirable, in order to take advantage of their full genetic yield potential. Phosphate greatly influences the yield of crop plants because its lack has detrimental effects on growth, flowering, fruit setting and ripening. Genetic modification however, requires knowledge of the molecules involved in this process. To this end we isolated three cDNAs from potato that showed homology to a high affinity phosphate transporter from Saccharomyces cerevisiae. This paper summarizes the characterisation of two of these clones so far, using yeast as an heterologous expression system and analysis of the genes at the transcriptional level. The results will be discussed in respect to more recent publications in this field. This summary is part of an oral presentation given at the “Sixth International Symposium on Genetics and Molecular Biology of Plant Nutrition” in Elsinore, Denmark.
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Literature cited
Alarcón, A.L., Madrid, R, and Egea, C. (1997) Hydric and nutrient element nutrition of a tomato crop on rockwool: Ionic interrelationships, J. Plant Nutr. 20, 1811–1828
Bun-ya, M., Nishimura, M., Harashima, S., and Oshima, Y. (1991) The PHO84 gene of Saccharomyces cerevisiae encodes an inorganic phosphate transporter, Mol. Cell. Biol. 11, 3229–3238.
Bun-ya, M., Harashima, S., and Oshima, Y. (1992) Putative GTP-binding protein, Gtrl, associated with the function of the PHO84 inorganic phosphate transporter in Saccharomyces cerevisiae. Mol. Cell. Biol. 12, 2958–2966.
Bun-ya, M., Shikata, K., Nakade, S., Yompakdee, C., Harashima, S., and Oshima, Y. (1996) Two new genes, PHO86 and PHO87, involved in inorganic phosphate uptake in Saccharomyces cerevisiae. Curr.Genet. 29, 344–351.
Clarkson, D.T., Hawkesford, M.J., Davidian, J.-C., and Grignon, C. (1992) Contrasting responses of sulphate and phosphate transport in barley (Hordeum vulgare L.) roots to protein-modifying reagents and inhibition of protein synthesis, Planta 187, 306–314.
Cogliatti, D.H., and Clarkson, D.T. (1983) Physiological changes in, and phosphate uptake by potato plants during development of, and recovery from phosphate deficiency, Physiol. Plant. 58, 287–294.
Daram, P., Brunner, S., Persson, B.L., Amrhein, N., and Bucher, M. (1998) Functional analysis and cell-specific expression of a phosphate transporter from tomato, Planta 206, 225–233.
Desbrosses-Fonrouges, A.-G., Chérel, I., Grignon, C., and Sentenac, H. (1998) Control of K’ channel activity: utilization of yeast to identify proteins interacting with AKT1, in: 11th International Workshop on Plant Membrane Biology, Cambridge 9–14th August 1998, Abstractbook, p131.
Forde, B.G., and Clarkson, D.T. Nitrate and ammonium nutrition of plants: Physiological and molecular perspectives, Adv. in Bot. Res. in press.
Harrison, M. and van Buuren, M.L. (1995) A phosphate transporter from the mycorrhizal fungus Glomus versiiforme, Nature 378, 626–629.
Kai, M., Masuda, Y., Kikuchi, Y., Osaki, M.,, and Tadano, T. (1997) Isolation and characterisation of a eDNA from Catharanthus roseus which is highly homologous with phosphate transporter, Soil Sci. Nutr. 43, 227–235.
Leggewie, G., Willmitzer, L, and Riesmeier, J.W. (1997) Two cDNAs from potato are able to complement a phosphate uptake-deficient yeast mutant: Identification of phosphate transporters from higher plants, Plant Cell 9, 381–392.
Liu, C., Muchal, U.S., Uthappa, M., Kononowicz, A.K., and Raghothama, K.G. (1998) Tomato phosphate transporter genes are differentially regulated in plant tissues by phosphorus, Plant Physiol. 116, 91–99.
Liu, H., Trieu, A.T., Blaylock, L.A., and Harrison, M.J. (1998) Cloning and characterisation of two phosphate transporters from Medicago truncatula roots: Regulation in response to phosphate and to colonization by arbuscular mycorrhizal (AM) fungi, MPMJ 11, 14–22.
Lu, Y.P., Zhen, R.G., and Rea, P.A. (1997) AtPT4: A fourth member of the Arabidopsis phosphate transporter gene family (Accession No. U97546) (PGR 97–082) Plant Physiol. 114, 747.
Martinez, P., and Persson, B.L. (1998) Identification, cloning and characterisation of a derepressible Na-coupled phosphate transporter in Saccharomyces cerevisiae, Mol. Gen. Genet. 258, 628–638.
Marschner, H. (1995) Mineral nutrition in plants, Ed2, Academic Press, San Diego, CA.
Mitsukawa, N., Okumura, S., Shirano, Y., Sato, S., Kato, L., Harashima, S., and Shibata, D. (1997) Overexpression of an Arabidopsis thaliana high-affinity phosphate transporter gene in tobacco cultured cells enhances cell growth under phosphate-limited conditions, Proc. Nat. Acad. Sci. USA 94, 7098–7102.
Muchal, U.S., Pardo, J.M., and Raghothama, K.G. (1996) Phosphate transporters from the higher plant Arabidopsis thaliana, Proc. Natl. Acad. Sci. USA 93, 10519–10523.
Poirier, Y., and Theunissen, H. (1995) Analysis of two proteins in Arabidopsis thaliana showing homology to animal Na+/Pi cotransporters, in: Program and abstracts, 10th International Workshop on Plant Membrane Biology, Regensburg, August 6–11, 1995, poster R34.
Schachtman, D.P., Reid, R.J., and Ayling S.M. (1998) Phosphorus uptake by plants: From soil to cell, Plant Physiol. 116, 447–453.
Schröppel-Meier, G., and Kaiser, W.M. (1988) Ion homeostasis in chloroplasts under salinity and mineral deficiency, Plant Physiol. 87, 828–832.
Smith, F.W., and Jackson, W.A. (1987a) Nitrogen enhancement of phosphate transport in roots of Zea mays L. I. Effects of ammonium and nitrate pretreatment, Plant Physiol. 84, 1314–1318.
Smith. F.W. and Jackson, W.A. (1987b) Nitrogen enhancement of phosphate transport in roots of Zea mays L. II. Kinetic and inhibitor studies, Plant Physiol. 84, 1319–1324.
Smith, F.W., Ealing, P.M., Hawkesford, M.J. and Clarkson, D.T. (1995) Plant members of a family of sulphate transporters reveal functional subtypes, Proc.Natl. Acad. Sci. USA 92, 9373–9377
Smith, F.W., Ealing, P.M., Dong, B., and Delhaize, E. (1997) The cloning of two Arabidopsis genes belonging to a phosphate transporter family, Plant J. 11, 83–92.
Yompakdee, C., Ogawa, N., Harashima, S., and Oshima, Y. (1996) A putative membrane protein, Pho88p, involved in inorganic phosphate transport in Saccharomyces cerevisiae, Mol. Gen. Genet. 251, 580–590
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Leggewie, G., Willmitzer, L., Riesmeier, J.W. (1999). Identification and Characterisation of Two CDNAs Encoding Phosphate Transporters from Solanum Tuberosum . In: Gissel-Nielsen, G., Jensen, A. (eds) Plant Nutrition — Molecular Biology and Genetics. Springer, Dordrecht. https://doi.org/10.1007/978-94-017-2685-6_17
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DOI: https://doi.org/10.1007/978-94-017-2685-6_17
Publisher Name: Springer, Dordrecht
Print ISBN: 978-90-481-5225-4
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