Abstract
Selectable marker genes are used in most plant transformation processes. Frequently, however, the selectable marker gene does not confer useful traits on the transgenic plant, and there are a number of advantages to removing the selectable marker gene after regeneration of the plant, including reducing the overall complexity of the transgene array, removal of redundant genetic elements, and improved regulatory and public acceptance, especially when antibiotic resistance marker genes are used as selectable markers. A number of site-specific recombinases of prokaryotic or yeast origin have been shown to function in transgenic plants for marker removal, including Cre/lox from bacteriophage P1 (Hoess and Abremski, 1990). We chose the Cre/lox system to develop technologies for marker removal in commercially important crop species.
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© 2003 Springer Science+Business Media Dordrecht
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Gilbertson, L. et al. (2003). Cre/lox Mediated Marker Gene Excision in Transgenic Crop Plants. In: Vasil, I.K. (eds) Plant Biotechnology 2002 and Beyond. Springer, Dordrecht. https://doi.org/10.1007/978-94-017-2679-5_43
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DOI: https://doi.org/10.1007/978-94-017-2679-5_43
Publisher Name: Springer, Dordrecht
Print ISBN: 978-90-481-6220-8
Online ISBN: 978-94-017-2679-5
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