Abstract
Very little is known about the regulation of fatty acid biosynthesis in the oil palm mesocarp which comprises 44% palmitic acid (C16:0), 39% oleic acid (C18:1) and less than 5% stearic acid (C18:0) in the commercial variety. Based on the fatty acid composition, it was postulated that relatively low activity of 13-ketoacyl ACP synthase (KAS) II and high activity of palmitoyl ACP thioesterase may account for the high level of palmitic acid. Screening of oil palm varieties having a range of fatty acid compositions showed strong positive correlation between KAS II activity and unsaturated fatty acid (C18:1 + C18:2 + C18:3) but negative correlation with palmitic acid thus confirming the postulation. The enzyme was purified 10000-fold by acetone extraction followed by CMSepharose, HR-DEAE cellulose, hydroxylapatite and ACP-Sepharose chromatography.
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References
MacKintosh, R.W., Hardie, D.G. and Slabas, A.R.: A new assay procedure to study the induction of P-ketoacyl ACP synthase I and II and the complete purification of (3ketoacyl ACP synthase I from developing seeds of oilseed rape (Brassicca napus), Biochim. Biophys. Acta. 1002 (1989), 114–124.
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© 1997 Springer Science+Business Media Dordrecht
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Ramli, U.S., Sambanthamurthi, R. (1997). ß-Ketoacyl-Acyl Carrier Protein [ACP] Synthase II in the Oil Palm (Elaeis Guineensis Jacq.) Mesocarp. In: Williams, J.P., Khan, M.U., Lem, N.W. (eds) Physiology, Biochemistry and Molecular Biology of Plant Lipids. Springer, Dordrecht. https://doi.org/10.1007/978-94-017-2662-7_22
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DOI: https://doi.org/10.1007/978-94-017-2662-7_22
Publisher Name: Springer, Dordrecht
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