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In Situ Hybridization in Plants — Methods and Application

  • Chapter
Molecular Techniques in Crop Improvement

Abstract

Cytogenetics is complementary to genetic and molecular analysis of plant genome structure and function. Since its beginning it has been mainly used for identification of chromosomes in plant genomes and karyotype construction (karyotyping). The cytological techniques applied to karyotyping should be easy, reproducible, and should be able to identify major structural rearrangements that can occur between chromosomes. Most significant for the progress in plant cytogenetics was the development of the squash method for chromosome preparation (Darlington, 1937), chromosome banding techniques (Caspersson et al., 1968) and in situ hybridization (ISH) techniques (Pardue and Gall, 1969), especially fluorescent in situ hybridization (FISH) (Pinkel et al., 1986). These methods have had a major impact on investigation and understanding of plant genome organisation.

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  1. Department of Plant Anatomy and Cytology, University of Silesia, Jagiellonska 28, 40-032, Katowice, Poland

    Jolanta Maluszynska

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  1. Jolanta Maluszynska
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  1. FAO/IAEA Joint Division, International Atomic Energy Agency, Vienna, Austria

    S. Mohan Jain

  2. International Rice Research Institute, Los Banos, Philippines

    D. S. Brar

  3. Agriculture and Food Development Authority, Dublin, Ireland

    B. S. Ahloowalia

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Maluszynska, J. (2002). In Situ Hybridization in Plants — Methods and Application. In: Jain, S.M., Brar, D.S., Ahloowalia, B.S. (eds) Molecular Techniques in Crop Improvement. Springer, Dordrecht. https://doi.org/10.1007/978-94-017-2356-5_11

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  • DOI: https://doi.org/10.1007/978-94-017-2356-5_11

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