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Use of rep-PCR to fingerprint the genome of Azospirillum spp.

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Molecular Microbial Ecology of the Soil

Part of the book series: Developments in Plant and Soil Sciences ((DPSS,volume 83))

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Abstract

The repetitive sequence based polymerase chain reaction (rep-PCR) was used to fingerprint the genomes of differentAzospirillum strains. Amplification products were produced using primers based on the repetitive element BOX (BOX-AIR), ERIC (ERIC IR, ERIC 2: Enterobacterial Repetitive Intergenic Consensus) and REP (REP IR, REP 2: Repetitive Extragenic Palindromic). The patterns of the resulting PCR products were analyzed on agarose gels. Band patterns generated with ERIC primers were unique for each strain and may be useful for the identification and classification of Azospirillum strains.

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© 1998 Springer Science+Business Media Dordrecht

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Mamaril, J.C., Trinidad, L.C. (1998). Use of rep-PCR to fingerprint the genome of Azospirillum spp.. In: Hardarson, G., Broughton, W.J. (eds) Molecular Microbial Ecology of the Soil. Developments in Plant and Soil Sciences, vol 83. Springer, Dordrecht. https://doi.org/10.1007/978-94-017-2321-3_15

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  • DOI: https://doi.org/10.1007/978-94-017-2321-3_15

  • Publisher Name: Springer, Dordrecht

  • Print ISBN: 978-90-481-5099-1

  • Online ISBN: 978-94-017-2321-3

  • eBook Packages: Springer Book Archive

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