Abstract
Total chromosomal DNAs from 20 Bradyrhizobium spp. strains (10 strains isolated from Vigna radiata and 10 from Arachis hypogaea) and 18 B. japonicum strains isolated from Glycine max were extracted. These DNAs served as templates for REP, ERIC and RAPD primers in PCR analyses. The patterns of the resulting PCR products were analyzed and highly specific for each strain, especially when grouped together with their antibiotic-resistance profiles. A method for extracting DNA directly from soil was developed. Recovery was approximately 30 µg DNA g−1 soil and the procedure yielded DNA suitable for PCR amplification.
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© 1998 Springer Science+Business Media Dordrecht
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Teaumroong, N., Boonkerd, N. (1998). Detection of Bradyrhizobium spp. and B. japonicum in Thailand by primer-based technology and direct DNA extraction. In: Hardarson, G., Broughton, W.J. (eds) Molecular Microbial Ecology of the Soil. Developments in Plant and Soil Sciences, vol 83. Springer, Dordrecht. https://doi.org/10.1007/978-94-017-2321-3_12
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DOI: https://doi.org/10.1007/978-94-017-2321-3_12
Publisher Name: Springer, Dordrecht
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