Abstract
Poor bean yields in the Cunha region of the Mata Atlântica ecosystem in the state of São Paulo, Brazil, are associated with low agronomic inputs, plant disease, and soil erosion. To identify sustainable farming practices that increase production and maximize biological N2 fixation (BNF), the effects of soil fertility and plant cultivar on seed yield and root nodule formation were measured under standard agronomic practices. Results from 16 sites showed that fertilizing with lime and molybdenum increased seed yields to 370% for the landrace Serro Azul. In addition to increased yields, plants grown with fertilizer had more nodules. Marked strains of Rhizobium tropici were tested under controlled environments. An indicator strain of Rhizobium containing the gusA marker gene was used. Our results verify that the indicator strain CM-255 GusA+Hup+ had a high capacity to associate with the five bean varieties tested. Fertilization with P, K, S + micronutrients and liming were essential for better nodulation by the indicator strain. Under low fertility conditions, the landrace variety Serro Azul was poorly nodulated, when associated with native strains or with the indicator strain. However, under better soil fertility conditions, nodulation of Serro Azul by the marked Rhizobium strain was increased. The commercial variety Carioca 80SH showed no increase in nodulation (nodule number).
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References
Barta A L 1982 Response of symbiotic N2 fixation and assimilate partitioning to K supply in alfalfa. Crop Sci. 22, 89–92.
Brady N C 1984 Soil formation, classification and survey. In The Nature and Properties of Soils. 9th ed. MacMillan Publishing Co, New York.
Brodrick S J, Sakala M K and Giller K E 1992 Molybdenum reserves of seed, and growth and N2 fixation by Phaseolus vulgaris L. Biol Fertil Soils 13, 39–44.
Cassman K G, Whitney A S and Fox R L 1981 Phosphorus requirements of soybean and cowpea as affected by mode of N nutrition. Agron. J. 73, 17–22.
Collins M and Duke S H 1981 Influence of potassium-fertilization rate and form on photosynthesis and N2 fixation of alfalfa. Crop Sci 21, 481–485.
Duke S H and Collins M 1985 Role of potassium in legume dinitrogen fixation. In: Potassium in Agriculture. Ed. Robert D, Munson. Am. Soc. Agron. pp 443–466.
Franco A A and Day J M 1980 Effects of lime and molybdenum on nodulation and nitrogen fixation of Phaseolus vulgaris L. in acid soils of Brazil. Turrialba 30, 990–1005.
Gates C T and Müller W J 1979 Nodule and plant development in the soybean, Glycine max (L) Merr.: Growth response to nitrogen, phosphorus and sulflur. Aust. J. Bot. 27, 203–215.
Gates C T and Wilson W J 1979 The interaction of nitrogen and phosphorus on the growth, nutrient status and nodulation of Stylosanthes humilis H.B.K. (Townsville Stylo). Plant Soil 41, 325–333.
Graham P H, Viteri S E, Mackie F, Vargas A A T, Palacios A 1982 Variation in acid soil tolerance among strain of Rhizobium phaseoli. Field Crops Res. 5, 121–128.
Graham R D 1983 Effects of nutrient stress on susceptibility of plants to disease with particular reference to the trace elements. Adv. Bot. Res. 10, 221–276.
Hernandez B S and Focht DD 1985 Effects of phosphorus, calcium, and Hup-and Hup-rhizobia on pigeon pea yields in an infertile tropical soil. Agron. J. 77, 867–871.
Lynd J G and Ansman T R 1989 Effects of P, Ca with four K levels on nodule histology, nitrogenase activity and improved spanto’ peanut yields. J. Plant Nutr. 12, 65–84.
Lynd J G and Ansman T R 1990 Soil conditions with distinctive coralloid nodulation and nitrogen fixation of `Mecca’ alfalfa. J. Plant Nutr. 13, 77–94.
Martínez-Romero E, Segovia L, Mercante F M, Franco A A, Graham P H and Pardo M A 1991 Rhizobium tropici, a novel species nodulating Phaseolus vulgaris and Leucaena sp. Trees. Int. J. Syst. Bacteriol. 41, 417–426.
Navarro R B, Vargas A A T, Schröder E C and van Berkum P 1993 Uptake hydrogenase (Hup) in common bean (Phaseolus vulgaris) symbioses. Appl. Environ. Microbiol. 59, 4161–4165.
Pereira P A A, Araujo R S, Moreira R G, Steinmets S 1984 Capacidade de genótipos de feijoeiro de fixar N2 atmosférico. Pesq. Agropecu. Bras. 19, 811–815.
Sessitsch A 1995 Manual for the Gus Gene Marking Kit. Joint FAO/IAEA Division, IAEA- Vienna, Austria. 37 p.
Streit W, Botero L, Werner D and Beck D 1995 Competition for nodule occupancy on Phaseolus vulgaris by Rhizobium etli and Rhizobium tropici strains can be efficiently monitored in an ultisol during the early stages of growth using a constitutive gus gene fusion. Biol. Fertil. Soils 27, 1075–1081.
Thung M 1991 Bean Agronomy in Monoculture in Common Beans: Research for Crop Improvement. Eds. A van Schoonhoven and O Voyset. CAB International, Wallingford.
Tsai S M, Bonetti R, Agbala S M and Rossetto R 1993 Minimizing the effect of mineral nitrogen on biological nitrogen fixation in common bean by increasing nutrient levels. Plant Soil 152: 131–138
Vieira R F 1994 Effects of rhizobial inoculation, N and Mo applications on the nitrogen nutrition of common bean (Phaseolus vulgaris L.). Dr. Dissertation, ESALQ/USP-Piracicaba — S.P., 189 p.
Wolyn D J, Attewell J, Ludden P W and Bliss F A 1989. Indirect measures of N2 fixation in common bean ( Phaseolus vulgaris L.) under field conditions: The role of lateral root nodules. Plant Soil 113, 181–187.
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© 1998 Springer Science+Business Media Dordrecht
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de Oliveira, W.S., Meinhardt, L.W., Sessitsch, A., Tsai, S.M. (1998). Analysis of Phaseolus-Rhizobium interactions in a subsistence farming system. In: Hardarson, G., Broughton, W.J. (eds) Molecular Microbial Ecology of the Soil. Developments in Plant and Soil Sciences, vol 83. Springer, Dordrecht. https://doi.org/10.1007/978-94-017-2321-3_10
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DOI: https://doi.org/10.1007/978-94-017-2321-3_10
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