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Molecular Diagnostics for Loma Salmonae and Nucleospora Salmonis (Microsporidia)

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Book cover Molecular Diagnosis of Salmonid Diseases

Part of the book series: Reviews: Methods and Technologies in Fish Biology and Fisheries ((REME,volume 3))

Abstract

Microsporidia are some of the smallest intracellular eukaryotic parasites. Some 14 genera of these ubiquitous spore-forming protists have been reported from fishes (Canning and Lom, 1986), with several causing disease in farmed and wild fish populations (reviewed in Shaw and Kent, 1999). Their tiny size (about 2–5 µm long), cryptic habitat within host cells, and difficulty with their in vitro culture necessitates development of molecular tools for highly sensitive and specific diagnostic tests. The traditional utility of electron microscopy for species identification of microsporidians is being superceded by immunological (Luna et al., 1995; Van Gool et al., 1993) and particularly PCR-based diagnostic tools (Da Silva et al., 1996; Hung et al., 1998; Khattra et al., 2000; Malone and McIvor, 1995; Sparfel et al., 1997; Velásquez et al., 1999; Zhu et al., 1993).

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Authors and Affiliations

  1. Department of Zoology, University of British Columbia, Vancouver, BC, Canada

    Amanda M. V. Brown

  2. Center for Salmon Disease Research, Oregon State University, Corvallis, OR, USA

    Michael L. Kent

Authors
  1. Amanda M. V. Brown
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  2. Michael L. Kent
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Editors and Affiliations

  1. FRS Marine Laboratory, Victoria Road, PO Box 101, AB11 9DB, Aberdeen, Scotland

    Carey O. Cunningham

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© 2002 Springer Science+Business Media Dordrecht

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Brown, A.M.V., Kent, M.L. (2002). Molecular Diagnostics for Loma Salmonae and Nucleospora Salmonis (Microsporidia). In: Cunningham, C.O. (eds) Molecular Diagnosis of Salmonid Diseases. Reviews: Methods and Technologies in Fish Biology and Fisheries, vol 3. Springer, Dordrecht. https://doi.org/10.1007/978-94-017-2315-2_10

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  • DOI: https://doi.org/10.1007/978-94-017-2315-2_10

  • Publisher Name: Springer, Dordrecht

  • Print ISBN: 978-90-481-5974-1

  • Online ISBN: 978-94-017-2315-2

  • eBook Packages: Springer Book Archive

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