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Genetic Engineering in Forest Trees: State of the Art and Future Perspectives

  • Chapter
Molecular Biology of Woody Plants

Part of the book series: Forestry Sciences ((FOSC,volume 64))

Abstract

Genetic engineering offers prospects of generating novel genotypes at an accelerated rate in the forest tree species. By employing Agrobacterium-mediated gene transfer system, a number of alien genes have been transferred to forest tree species (Fillatti et al. 1987; Klopfenstein et al. 1993; Nilsson et al. 1996; Tzfira et al. 1996; Fladung et al. 1997a; Holland et al. 1997). In addition, gene transfer has also been accomplished in the woody plants by biolistic DNA delivery system (Ellis et al. 1993; Charest et al. 1996; Walter et al. 1998a). Forest trees have long generation cycles with vegetative phases extending from one to several decades. Therefore, it is relevant to ask if the foreign genes would be stably integrated and expressed in the forest trees on a short-term as well as long-term basis (Ahuja, 1988a, 1988b; 1997). Genetic and phenotypic stability of transgenic trees is important for subsequent utility in the commercial forestry. In this direction, it would be desirable to isolate transgenic lines that are capable of moderate to high expression of the transgenes, and remain stable following vegetative and sexual reproduction. The transgenic plants must maintain a high degree of transgene expression during growth in the greenhouse, but most importantly under field conditions.

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Ahuja, M.R. (2000). Genetic Engineering in Forest Trees: State of the Art and Future Perspectives. In: Jain, S.M., Minocha, S.C. (eds) Molecular Biology of Woody Plants. Forestry Sciences, vol 64. Springer, Dordrecht. https://doi.org/10.1007/978-94-017-2311-4_2

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