Abstract
Polycrystalline diamond surfaces have attractive characteristics for bioanalysis, such as the highest thermal conductivity, optical transparency, chemical inertness, and by choice, electrical insulation or semiconduction. This paper is one step in making diamond microfluidic systems for e.g. chromatography.
With a silicon-based microengineering technology, diamond microchips with 40 mm straight columns for chromatography were fabricated. The channels of the diamond chips were filled with continuous polymer beds and used for anion-exchange chromatography of proteins. A sample of myoglobin (horse) conalbumin, ovalbumin (chicken), and trypsin inhibitor (soybean) was separated in about 30 s and a sample of Hemoglobin A0 and β-lactoglobulin A was separated in less than 20 s. The continuous bed was found to be uniformly polymerized, also close to the inner wall, and the resolution of the chromatograms were comparable with separations on continuous beds synthesized in both fused-silica capillaries and quartz microchips.
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© 2000 Springer Science+Business Media Dordrecht
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Björkman, H., Ericson, C., Hjertén, S., Hjort, K. (2000). Diamond Microchip Capillary Chromatography of Proteins. In: van den Berg, A., Olthuis, W., Bergveld, P. (eds) Micro Total Analysis Systems 2000. Springer, Dordrecht. https://doi.org/10.1007/978-94-017-2264-3_44
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DOI: https://doi.org/10.1007/978-94-017-2264-3_44
Publisher Name: Springer, Dordrecht
Print ISBN: 978-90-481-5496-8
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