Abstract
Flow cytometry was demonstrated on a glass microchip fabricated using standard photolithographic techniques. The channel walls were coated to prevent cell adhesion, and the cells were transported electrophoretically by applying potentials to the fluid reservoirs. The sample stream was electrophoretically focused in two dimensions at a cross intersection to enable single cell interrogation. An Escherichia coli sample was labeled on-chip with a membrane permeable nucleic acid stain Syto 15, and coincident light scattering and fluorescence data were obtained from the these labeled cells.
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References
H.M. Shapiro, “Practical flow cytometry,” Wiley-Liss, New York, (1995).
G. Blankenstein and U.D. Larsen, “Modular concept of a laboratory on a chip for chemical and biochemical analysis,” Biosensors and Bioelectronics, 13, (1998), 427–438.
G. Blankenstein, L. Scampavia, J. Ruzicka, and G.D. Christian, “Coaxial flow mixer for real time monitoring of cellular responses in flow injection cytometry,” Cytotnetry, 13, (1996), 200–204.
A.Y. Fu, C. Spence, A. Scherer, F.H. Arnold, and S.R. Quake, “A microfabricated fluorescence-activated cell sorter,” Nature Biotechnology,17, (1999), 1109–1111.
P.C.H. Li and D.J. Harrison, “Transport, manipulation and reaction of biological cells on-chip using electrokinetic effects,” Analytical Chemistry,69, (1997), 1564–1568.
S.C. Jacobson and J.M. Ramsey, “Electrokinetic focusing in microfabricated channel structures,” Analytical Chemistry, 69, (1997), 3212–3217.
D.P. Schrum, C.T. Culbertson, S.C. Jacobson, and J.M. Ramsey, “Microchip flow cytometry using electrokinetic focusing,” Analytical Chemistry, 71, (1999), 4173–4177.
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© 2000 Springer Science+Business Media Dordrecht
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Jacobson, S.C., McClain, M.A., Culbertson, C.T., Ramsey, J.M. (2000). Microfabricated Fluidic Devices for Cellular Assays. In: van den Berg, A., Olthuis, W., Bergveld, P. (eds) Micro Total Analysis Systems 2000. Springer, Dordrecht. https://doi.org/10.1007/978-94-017-2264-3_24
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DOI: https://doi.org/10.1007/978-94-017-2264-3_24
Publisher Name: Springer, Dordrecht
Print ISBN: 978-90-481-5496-8
Online ISBN: 978-94-017-2264-3
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