Abstract
Counting and sizing of biological cells has been used for half a century as a tool in health care and food quality control. This paper describes the employment of a method known as “Coulter sizing” which is based on a small aperture in a membrane. The possibility of microfabrication of silicon provides a cheap and better alternative to current day technologies of aperture manufacture. Silicon technology allows for the aperture to become cheap enough so it can be used as a disposable unit in a Coulter counter. We have manufactured and investigated silicon apertures with different sizes for sizing and counting of particles. One of the places where a simple particle counter would be applicable is on diary farms, where somatic cell counts (SCC) are used for quality control.
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References
A. Y. Fu et al, “A microfabricated fluorescence-activated cell sorter”, Nature Biotechnology, Vol. 17, November 1999, pp. 1109–1111
V. Kachel,“Electrical Resistance Pulse Sizing: Coulter Sizing”, Flow cytometry and sorting, Second edition, pages 45–80, 1990 Wiley-Liss, Inc.
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© 2000 Springer Science+Business Media Dordrecht
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Larsen, U.D., Nørring, H., Telleman, P. (2000). Somatic Cell Counting with Silicon Apertures. In: van den Berg, A., Olthuis, W., Bergveld, P. (eds) Micro Total Analysis Systems 2000. Springer, Dordrecht. https://doi.org/10.1007/978-94-017-2264-3_23
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DOI: https://doi.org/10.1007/978-94-017-2264-3_23
Publisher Name: Springer, Dordrecht
Print ISBN: 978-90-481-5496-8
Online ISBN: 978-94-017-2264-3
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