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Microflow Analysis Based on Seqential and Bead Injection

  • Conference paper
Book cover Micro Total Analysis Systems 2000

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Abstract

While use of the continuous forward flow is essential for success of chromatographic separations, its use for automation of the reagent-based assays has numerous drawbacks. This is why the first generation of flow injection (FI) methodology is presently being replaced by sequential injection (SI) that uses reversed/stop/forward flow format, and by bead injection (BI) that employs micro spheres as reagent carriers as well as sensing and separation medium. This communication introduces these two novel concepts as well as the device for their implementation into the toolbox of µTAS technology. The device, nicknamed “ Lab-on-Valve” is a multipurpose central sample processing unit (CSPU) served by a conventional sized peripherals. The performance of the system, configured for UV-VIS and fluorescence spectroscopy is characterized by dye injection and its performance is demonstrated on enzymatic reaction rate assay of glucose and Bioligand Interaction assay of anti-mouse IgG with recombinant protein G.

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References

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Author information

Authors and Affiliations

  1. Department of Chemistry, University of Washington, Seattle, WA, 98115, USA

    Jaromir Ruzicka

Authors
  1. Jaromir Ruzicka
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Editor information

Editors and Affiliations

  1. MESA+ Research Institute, University of Twente, Enschede, The Netherlands

    Albert van den Berg (Conference Chairman), W. Olthuis  & Piet Bergveld (Conference Chairman),  & 

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© 2000 Springer Science+Business Media Dordrecht

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Ruzicka, J. (2000). Microflow Analysis Based on Seqential and Bead Injection. In: van den Berg, A., Olthuis, W., Bergveld, P. (eds) Micro Total Analysis Systems 2000. Springer, Dordrecht. https://doi.org/10.1007/978-94-017-2264-3_1

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  • DOI: https://doi.org/10.1007/978-94-017-2264-3_1

  • Publisher Name: Springer, Dordrecht

  • Print ISBN: 978-90-481-5496-8

  • Online ISBN: 978-94-017-2264-3

  • eBook Packages: Springer Book Archive

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