Abstract
Laboratory studies of bacterial gene expression have not allowed the investigation of in vivo factors crucial to pathogenesis. Recently, genetic tools have been used to identify in vivo-activated genes. These tools, however, have not allowed the measurement of specific expression levels of virulence-associated bacterial genes in vivo. This report describes a system, previously tested utilizing the fructosyltransferase (ftf)gene of Streptococcus mutans, that allows the measurement of activity of specific virulence-associated streptococcal genes while bacteria are living in the animal environment.
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© 1998 Springer Science+Business Media Dordrecht
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Grey, W.T., Lasker, J.D., Curtiss, R., Hudson, M.C. (1998). Examination of streptococcal gene expression in the mammalian environment. In: Fives-Taylor, P.M., LeBlanc, D.J. (eds) Methods for studying the genetics, molecular biology, physiology, and pathogenesis of the streptococci. Springer, Dordrecht. https://doi.org/10.1007/978-94-017-2258-2_16
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DOI: https://doi.org/10.1007/978-94-017-2258-2_16
Publisher Name: Springer, Dordrecht
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