Protoplast Fusion and DNA Plasmid Characterisation in Rhizoctonia Solani
- 378 Downloads
Protoplasts have been used routinely for physiological and biological investigations. More recently, protoplasts have been frequently used for genetic studies. Two methods have been developed for introducing favorable genes into microbial protoplasts (Figure 1). One method, protoplast fusion (Figure 1) serves as a convenient method for introducing genes from one fungal isolate to another. The protoplast fusion process has been shown to generate a large number of random recombinants. Furthermore, because the protoplast fusion is observed not only among different strains but also different species, this method of gene transfer may be advantageous to improve fungal strains. The other method, genetic transformation of protoplasts by the uptake of recombinant DNA (Figure 1) is widely used.
KeywordsMinimal Medium Protoplast Fusion Anastomosis Group Linear Plasmid Auxotrophic Mutant
Unable to display preview. Download preview PDF.
- Davis, B. (1985) Factors influencing protoplast isolation, in J. F. Peberdy and L. Ferenczy (eds.), Fungal protoplasts. Application in biochemistry and genetics, New York, Marcel Dekker, Inc., pp.45–72. FerenczyGoogle Scholar
- Hashiba, T. (1992) Isolation of fungal protoplast, in D. K. Arora, R. P. Elander, and K. G. Mukerji (eds.), Handbook of applied mycology vol. 4. Fungal biotechnology, New York, Marcel Dekker, Inc., pp.129–148.Google Scholar
- Hashiba, T., Homma, Y., Hyakumachi, M. and Matsuda, I. (1984) Isolation of a DNA plasmid in the fungus Rhizoctonia solani, Jounal of General Microbiology 130, 2067–2070.Google Scholar
- Katsura, K., Suzuki, F., Miyashita, S., Nishi, T., Hirochika, H. and Hashiba, T. (1998) The complete nucleotide sequence and characterization of the linear DNA plasmids pRS64 from the plant pathogenic fungus Rhizoctonia solani, Current Genetics. (in press)Google Scholar
- Wako, T., Ishikawa, T. and Hashiba, T. (1991) Unique DNA plasmid pRS64 associated with chromosomal DNAs of the plant pathogenic fungus Rhizoctonia solani, Journal of General Microbiology 137, 2817–2821.Google Scholar