Protoplast Fusion and DNA Plasmid Characterisation in Rhizoctonia Solani

  • T. Hashiba
  • A. Sasaki
  • K. Katsura


Protoplasts have been used routinely for physiological and biological investigations. More recently, protoplasts have been frequently used for genetic studies. Two methods have been developed for introducing favorable genes into microbial protoplasts (Figure 1). One method, protoplast fusion (Figure 1) serves as a convenient method for introducing genes from one fungal isolate to another. The protoplast fusion process has been shown to generate a large number of random recombinants. Furthermore, because the protoplast fusion is observed not only among different strains but also different species, this method of gene transfer may be advantageous to improve fungal strains. The other method, genetic transformation of protoplasts by the uptake of recombinant DNA (Figure 1) is widely used.


Minimal Medium Protoplast Fusion Anastomosis Group Linear Plasmid Auxotrophic Mutant 
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Copyright information

© Springer Science+Business Media Dordrecht 2001

Authors and Affiliations

  • T. Hashiba
    • 1
  • A. Sasaki
    • 1
  • K. Katsura
    • 1
  1. 1.Faculty of AgricultureTohoku UniversitySendai 981Japan

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