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In Vitro Haploid Production in Higher Plants pp 217–235Cite as

Ovary and ovule culture for haploid production

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Part of the book series: Current Plant Science and Biotechnology in Agriculture ((PSBA,volume 23))

Abstract

The first natural haploid was reported in Datura stramonium (Blakeslee et al., 1922). There are only a few examples of spontaneous production of haploids in field culture of tomato and commercial strains of cotton (Kimber and Riley, 1963). Because of the potential of haploids in breeding and genetic studies, much effort has been made to induce haploid sporophytes by various physical, chemical and biological stimulants or via in vitro culture. In vitro culture of unfertilized ovaries and ovules has been tried without success with Cooperia pedunculata (Sachar and Kapoor, 1958), Zephyranthes (Sachar and Kapoor, 1959) and Papaver somniferum (Maheshwari, 1958; Maheshwari and Lal, 1961). Under conditions of in vitro culture, ovules with the zygote and primary endosperm nucleus developed only to the globular stage of embryo development. The formation of endosperm was also inhibited.

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References

  • Ahmim, M. and J. Vieth, 1986. Production de plantes haploïdes de Gerbera jamesonii par culture in vitro d’ovules. Can. J. Bot. 64: 2355–2357.

    Google Scholar 

  • Asselin de Beauville, M., 1980. Obtention d’haploïdes in vitro à partir d’ovaires non fecondés de riz, Oryza sativa L. C.R. Acad. Sci. Paris 290: 489–492.

    Google Scholar 

  • Badea, E.M., M. Prisecaru and R. Angheluta, 1989. Studies on gynogenesis in intraspecific and interspecific hybrids in the genus Helianthus. Cercetary de Genetica Vegetala si Animala 1: 177–183.

    Google Scholar 

  • Barclay, I.R., 1975. High frequencies of haploid production in wheat (Triticum aestivum) by chromosomal elimination. Nature 256: 410–411.

    Article  Google Scholar 

  • Blakeslee, A.F., J. Belling, M.E. Farnham and A.D. Bergner, 1922. A haploid mutant in the Jimson weed, Datura stramonium. Science 55: 646–647.

    Article  PubMed  CAS  Google Scholar 

  • Bornman, C.H., 1985. Haploidization of sugar beet (Beta vulgaris) via gynogenesis. In Vitro 1: 3.

    Google Scholar 

  • Bossoutrot, D and D. Hosemans, 1985. Gynogenesis in Beta vulgaris L.: From in vitro culture of unpollinated ovules to the production of doubled haploid plants in soil. Plant Cell Rep. 4: 300–303.

    Google Scholar 

  • Bossoutrot, D. and D. Hosemans, 1986. Gynogénèse chez Beta vulgaris L. In: Obtention de haploïdes in vitro: état actuel et perspectives. Colloque de la section française de l’IAPTC, Orsay, 1985, April 30, Bull. Soc. Bot. France, Act. Bot. 133: 79.

    Google Scholar 

  • Bugara, A.M. and L.V. Rusina, 1989. Haploid callus formation in the culture of unfertilized ovules of clary sage. Fiziol. Biokh. Kul’t. Rast. 20: 554–560.

    Google Scholar 

  • Campion, B. and C. Alloni, 1990. Induction of haploid plants in onion (Allium cepa L.) by in vitro culture of unpollinated ovules. Plant Cell Tissue Organ Cult. 20: 1–6.

    Google Scholar 

  • Campion, B. and M T Azzimonti, 1988. Evolution of ploidy level in haploid plants of onion (Album cepa) obtained through in vitro gynogenesis. In: 4th EUCARPIA Allium Symposium, Wellesbourne (UK), September 6–9, 1988, pp. 85–89.

    Google Scholar 

  • Campion, B., M.T. Azzimonti, E. Vicini, M. Schiavi and A. Falavigna, 1992. Advances in haploid plant induction in onion (Allium cepa L.) through in vitro gynogenesis. Plant Sci. 86: 97–104.

    Article  Google Scholar 

  • Cappadocia, M., L. Chretien and G. Laublin, 1988. Production of haploids in Gerbera jamesonii via ovule culture: influence of fall versus spring sampling on callus formation and shoot regeneration. Can. J. Bot. 66: 1107–1110.

    Article  Google Scholar 

  • Castillo, A.M. and L. Cistué, 1993. Production of gynogenic haploids of Hordeum vulgare L. Plant Cell Rep. 12: 139–143.

    Article  CAS  Google Scholar 

  • Chambonnet, D. and R. Dumas de Vaulx, 1985. Obtention of embryos and plants from in vitro culture of unfertilized ovules of Cucurbita pepo. Rep. Cucurbit Genetics Cooperative USA 8: 66.

    Google Scholar 

  • Chen, Z.H., W.B. Li, L.H. Zhang, X.E. Xu and S.J. Zhang, 1988. Production of haploid plantlets in cultures of unpollinated ovules of Hevea brasiliensis Muell.-Arg. In: M.R. Ahuja (Ed.), Proc. IUFRO Working Party S2.04–07, Grosshansdorf, FRG, August 10–13, 1987, pp. 39–44. Kluwer Academic Publishers, Dordrecht.

    Google Scholar 

  • Clapham, D., 1971. In vitro development of callus from the pollen of Lolium and Hordeum. Z. Pflanzenzüchtg. 65: 285–292.

    Google Scholar 

  • Cuny, F., R. Dumas de Vaulx, B. Longhi and R. Siadous, 1992. Analyse des plantes de melon (Cucumis melo L.) issues de croisements avec du pollen irradié à différentes doses. Agronomie 12: 623–630.

    Article  Google Scholar 

  • Cuny, F., M. Grotte, R. Dumas de Vaulx and A. Rieu, 1993. Effects of gamma irradiation of pollen on parthenogenetic haploid production in muskmelon (Cucumis melo L.). Environ. Exp. Bot. 33: 301–312.

    Article  Google Scholar 

  • Darlington, C.D., 1937. Recent Advances in Cytology, 2nd Ed. J.A. Churchill Ltd., London. Davoyan, E.I., 1985. Obtaining haploids in rice by means of in vitro culture of unfertilized ovaries. Doklady Vses. Akad. S.-h. Nauk Im. Lenina 3: 15–16.

    Google Scholar 

  • Deuter, M., 1970. Some remarks on methods for the establishment of the degree of ploidy in sugar beet. Genet. Pol. 11: 219–225.

    Google Scholar 

  • D’Halluin, K. and B. Keimer, 1986. Production of haploid sugarbeets (Beta vulgaris L.) by ovule culture. In: W. Horn, C.J. Jensen, W. Odenbach and O. Schieder (Eds.), Proc. Intl. Symp. EUCARPIA. September 8–13, 1985, Berlin, pp. 307–309.

    Google Scholar 

  • Doctrinal, M., R.S. Sangwan and B.S. Sangwan-Norreel, 1989. In vitro gynogenesis in Beta vulgaris L.: effects of plant growth regulators, temperature, genotypes and season. Plant Cell Tissue Organ Cult. 17: 1–12.

    Google Scholar 

  • Doré, C., 1986. Evaluation du niveau de ploïdie des plantes d’une population de choux de Bruxelles (Brassica oleracea L. ssp. gemmifera) d’origine pollinique. Agronomie 6: 797–801.

    Article  Google Scholar 

  • Doré, C., 1989. Obtention de plantes haploïdes de chou cabus (Brassica oleracea L. ssp. capitata) après culture in vitro d’ovules pollinisés par du pollen irradié. C.R. Acad. Sci. Paris 309: 729–734.

    Google Scholar 

  • Doré, C., L. Bulldard, A. Sauton, J.-C. Rode, F. Cuny, K. Niemirowicz-Szczytt, N. Sari and R. Dumas de Vaulx, 1994. Interest of irradiated pollen for obtaining haploid vegetables. In: Proc. XXIV. Intl. Hortic. Congress, Kyoto, 1994 (in press).

    Google Scholar 

  • Dumas de Vaulx. R., 1979. Obtention de plantes haploïdes chez le melon (Cucumis melo L.) après pollinisation par Cucumis ficifolius A. Rich. C.R. Acad. Sci. Paris 289: 875–878.

    Google Scholar 

  • Dunstan, D.I. and K.S. Short, 1977. Improved growth of tissue cultures of the onion, Allium cepa. Physiol. Plant. 41: 70–72.

    Article  Google Scholar 

  • Enaleeva, N.K. and N.A. Dushaeva, 1975. Cytoembryological studies in Nicotiana tabacum L. by means of enzymatic maceration. In: Apomixis and Cytoembryology of Plants, Vol. 3, pp. 171–175. Saratov Univ. Press, Saratov.

    Google Scholar 

  • Ferrant, V. and Bouharmont, 1994. Origin of gynogenic embryos of Beta vulgaris L. Sex. Plant Reprod. 7: 12–16.

    Article  Google Scholar 

  • Galatowitsch, M.W. and G.A. Smith, 1990. Regeneration from unfertilized ovule callus of sugarbeet (Beta vulgaris L.). Can. J. Plant Sci. 70: 83–89.

    Article  Google Scholar 

  • Gamborg, O.L., R.A. Miller and K. Ojima, 1968. Nutrient requirements of suspension cultures of soybean root cells. Exp. Cell Res. 50: 157–158.

    Article  Google Scholar 

  • Gelebart, P., 1986. Obtention de plantes haploïdes par culture in vitro d’ovaires non fecondés d’Helianthus annuus L. Bull. Soc. Bot. France. Act. Bot. 133: 4, 71.

    Google Scholar 

  • Gelebart, P. and L.H. San, 1987. Obtention de plantes haploïdes par culture in vitro d’ovaires et d’ovules non fecondés de tournesol (Helianthus annuus). Agronomie 7: 81–86.

    Article  Google Scholar 

  • Gu, Z.P. and K.C. Cheng, 1983. In vitro induction of haploid plantlets from unpollinated young ovaries of lily and embryological observations. Acta Bot. Sin. 25: 24–28.

    Google Scholar 

  • Gu, Z.P. and K.C. Cheng, 1984. In vitro induction of haploid plantlets from unpollinated ovaries of highland barley. Acta Bot. Sin. 26: 549–551.

    Google Scholar 

  • Guha, S. and S.C. Maheshwari, 1964. In vitro production of embryos from anthers of Datura. Nature (London) 204: 497.

    Google Scholar 

  • Gürsöz, N., K. Abak, M. Pitrat, J.-C. Rode and R. Dumas de Vaulx, 1991. Obtention of haploid plants induced by irradiated pollen in watermelon (Citrullus lanatus). Cucurbitae Genetics Coop. 14: 109.

    Google Scholar 

  • Gusakovskaya, M.A. and M.A. Nadzhar, 1994. Gynogenesis in the culture of unfertilized wheat ovaries and ovules. Bot. Zum. 79: 70–79.

    Google Scholar 

  • Hayase, H., 1954. Cucurbita crosses. Occurrence of a haploid twin pair from a F1 progeny of C. maxima x C. moschata. Jpn. J. Breed. 4: 55.

    Google Scholar 

  • Hosemans, D. and D. Bossoutrot, 1983. Induction of haploid plants from in vitro culture of unpollinated beet ovules (Beta vulgaris). Z. Pflanzenzüchtg. 91: 74–77.

    Google Scholar 

  • Herr, J.M. Jr., 1971. A new clearing-squash technique for the study of ovule development in angiosperms. Am. J. Bot. 58: 785–790.

    Article  Google Scholar 

  • Huang, B.Q. and S.D. Russel, 1989. Isolation of fixed and viable eggs, central cells, and embryo sacs from ovules of Plumbago zeylanica. Plant Physiol. 90: 9–12.

    Article  PubMed  CAS  Google Scholar 

  • Inagaki, M.N., 1990. Wheat haploids through the Bulbosum technique. In: Y.P.S. Bajaj (Ed.), Biotechnology in Agriculture and Forestry. Vol. 13. Wheat, pp. 448–459. Springer-Verlag, Berlin/Heidelberg/New York.

    Google Scholar 

  • Kasha, K.J. and K.N. Kao, 1970. High frequency haploid production in barley (Hordeum vulgare L.). Nature 225: 874–876.

    Article  PubMed  CAS  Google Scholar 

  • Kasha, K.J. and E. Reinbergs, 1982. Recent development in the production and utilization of haploids in barley. Barley Genetics 4: 653–665.

    Google Scholar 

  • Keller, J. 1990a. Results of anther and ovule culture in some species and hybrids in the genus Allium L. Arch. Züchtungsforsch. 20: 189–197.

    Google Scholar 

  • Keller, J., 1990b. Culture of unpollinated ovules, ovaries, and flower buds in some species of the genus Allium and haploid induction via gynogenesis in onion (Allium cepa L). Euphytica 47: 241–247.

    Google Scholar 

  • Keller, J., 1990c. Haploids from unpollinated ovaries of Allium cepa - single plant screening, haploid determination, and long term storage. In: H.J.J. Nijkamp, L.H.W. van der Plas, J. van Aartrijk (Eds.), Progress in Plant Cellular and Molecular Biology. Proc. VII. Intl. Congr. Plant Tissue and Cell Cult., Amsterdam, June 24–29, 1990, pp. 275–279. Kluwer Academic Publishers, Dordrecht.

    Google Scholar 

  • Keller, E.R.J., D.-E. Lesemann, H.I. Maaß, A. Meister, H. Lux and I. Schubert, 1995. Maintenance of an in vitro collection of Allium in the Gatersleben genebank–problems and use. In: M. Terzi, R. Cella, A. Falavigna (Eds.), Current Issues in Plant Molecular and Cellular Biology, Proc. VIIIth Intl. Congr. Plant Tissue Cell Cult., Florence, June 12–17, 1994, pp. 347–352. Kluwer Academic Publishers, Dordrecht.

    Chapter  Google Scholar 

  • Kimber, G. and R. Riley, 1963. Haploid angiosperms. Bot. Rev. 29: 480–531.

    Article  Google Scholar 

  • Kumashiro, T. and T. Oinuma, 1985. Comparison of genetic variability among anther-derived and ovule-derived doubled haploid lines of tobacco. Jpn. J. Breed. 35: 301–310.

    Google Scholar 

  • Kuo, C.S., 1982. The preliminary studies on culture of unfertilized ovary of rice in vitro. Acta Bot. Sin. 24: 33–38.

    Google Scholar 

  • Kuo, C.S., 1983. The culture of unfertilized rice ovaries. In: Cell and Tissue Culture Techniques for Cereal Crop Improvement, p. 438. Science Press, Bejing, IRRI, Manila.

    Google Scholar 

  • Li, M.W. and B. Zhang, 1984. In vitro induction of haploid plantlets from unpollinated ovaries of Coix lacryma-jobi L. Hereditas, China 6: 5–6.

    Google Scholar 

  • Litz, R.E., 1991. Cultivo de embriones y ovulos. In: W.M. Roca and L.A. Mroginski (Eds.), Cultivo de Tejidos en la Agricultura: Fundamentos y Aplicaciones, CIAT Publ. No. 151, pp. 295–312.

    Google Scholar 

  • Liu, Z.L. and C. Zhou, 1984. Investigation of ploidy and other characters of gynogenic plants in Oryza sativa L. Acta Genet. Sin. 11: 113–119.

    Google Scholar 

  • Lu, W.Z. and G.N. Wu, 1986. Current development of biotechnology and its applications to plant breeding in China. J. Agric. Sci. China. 2, Suppl.: 1–9.

    Google Scholar 

  • Lux, H., L. Herrmann and C. Wetzel, 1990. Production of haploid sugar beet (Beta vulgaris L.) by culturing unpollinated ovules. Plant Breed. 104: 177–183.

    Article  Google Scholar 

  • Maheshwari, N., 1958. In vitro culture of excised ovules of Papaver somniferum L. Science 127: 342.

    Google Scholar 

  • Maheshwari, N. and M. Lal, 1961. In vitro culture of excised ovules of Papaver somniferum L. Phytomorphology 11: 307–314.

    Google Scholar 

  • Meynet, J., R. Barrade, A. Duclos and R. Siadous, 1994. Dihaploid plants of roses (Rosa x hybrida, cv. “Sonia”) obtained by parthenogenesis induced using irradiated pollen and in vitro culture of immature seeds. Agronomie 14: 169–175.

    Article  Google Scholar 

  • Meynet, J. and M. Sibi, 1984. Haploid plants from in vitro culture of unfertilized ovules in Gerbera jamesonii. Z. Pflanzenzüchtg. 93: 78–85.

    Google Scholar 

  • Miller, C.O., 1963. Kinetin and kinetin-like compounds. In: H.F. Linskens and M.V. Tracey (Eds.), Moderne Methoden der Pflanzenanalyse. Vol. 6, pp. 194–202. Springer-Verlag, Berlin.

    Google Scholar 

  • Mix, G., 1985. Antheren-und Ovarkultur von Sonnenblumen (Helianthus annuus L.). Landbau-forsch. Völkenrode. 35: 153–156.

    Google Scholar 

  • Mól, R., 1992. In vitro gynogenesis in Melandrium album: from parthenogenetic embryos to mixoploid plants. Plant Sci. 81: 261–269.

    Google Scholar 

  • Murashige, T. and F. Skoog, 1962. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant. 15: 473–495.

    Article  CAS  Google Scholar 

  • Muren, R.C., 1989. Haploid plant induction from unpollinated ovaries in onion. HortScience 24: 833–834.

    Google Scholar 

  • Niemirowicz-Szcytt, K. and R. Dumas de Vaulx, 1989. Preliminary data on haploid cucumber (Cucumis sativus L.) induction. Cucurbitae Genetics Coop. 12: 24.

    Google Scholar 

  • Nikitina, N.I., T.S. Fedorenko and A.Y. Borovkov, 1989. Culture of unfertilized ovules in sunflower. Nauchn. Techn. Bjull. Vsesoyuzn. Nauchn.-Issledovatel’sk. Inst. Maslichnykh Kul’tur 3: 14–16.

    Google Scholar 

  • O’Donoughue, L.S. and M.D. Bennett, 1994. Durum wheat haploid production using maize wide-crossing. Theor. Appl. Genet. 89: 559–566.

    Google Scholar 

  • Pickering, R.A. and A.R. Wallace, 1994. Gibberellic acid + 2,4-D improves seed quality in Hordeum vulgare L. x H. bulbosum L. crosses. Plant Breed. 113: 174–176.

    Article  CAS  Google Scholar 

  • Raquin, C., 1985. Induction of haploid plants by in vitro culture of petunia ovaries pollinated with irradiated pollen. Z. Pflanzenzüchtg. 94: 166–169.

    Google Scholar 

  • Rode, J.C. and R. Dumas de Vaulx, 1987. Obtention de plantes haploïdes de carotte (Daucus carota L.) issues de parthénogénèse induite in situ par du pollen irradié et culture in vitro des graines immatures. C.R. Acad. Sci. Paris 305: 225–229.

    Google Scholar 

  • Sachar, R.C. and M. Kapoor, 1958. Influence of kinetin and gibberellic acid on the test tube seeds of Cooperia pedunculata Herb. Naturwissenschaften 45: 552–553.

    Article  CAS  Google Scholar 

  • Sachar, R.C. and M. Kapoor, 1959. In vitro culture of ovules of Zephyranthes. Phytomorphology 9: 147–156.

    Google Scholar 

  • San, L.H. and Y. Demarly, 1984. Gynogenesis in vitro and biometric studies of doubled haploids obtained by three techniques in Hordeum vulgare L. In: W. Lange, A.C. Zeven and N.G. Hogenboom (Eds.), Efficiency in Plant Breeding. Proc. 10th. Congr. EUCARPIA, Wageningen, June 19–24, 1983, p. 347.

    Google Scholar 

  • San Noeum, L.H., 1976. Haploïdes d’Hordeum vulgare L. par culture in vitro d’ovaires non fecondés. Ann. Amélior. Plantes 26: 751–754.

    Google Scholar 

  • San Noeum, L.H., 1979. In vitro induction of gynogenesis in higher plants. In: A.M. van Harten and A.C. Zeven (Eds.), Proc. Conf. Broadening the Genetic Base of Crops, Wageningen, July 3–7, 1978, pp. 327–329. Pudoc, Wageningen.

    Google Scholar 

  • Sauton, A., 1988. Effect of season and genotype on gynogenetic haploid production in muskmelon (Cucumis melo L.). Sci. Hortic. 35: 71–75.

    Article  Google Scholar 

  • Sauton, A. and R. Dumas de Vaulx, 1987. Obtention de plantes haploïdes chez le melon (Cucumis melo L.) par gynogénèse induite par du pollen irradié. Agronomie 7: 141–148.

    Article  Google Scholar 

  • Sitbon, M., 1981. Production of haploid Gerbera jamesonii plants by in vitro culture of unfertilized ovules. Agronomie 1: 807–812.

    Google Scholar 

  • Speckmann, G.J. Jr., J.P.C. van Geyt and M. Jacobs, 1986. The induction of haploids of sugarbeet (Beta vulgaris L.) using anther and free pollen culture or ovule and ovary culture. In: W. Horn, C.J. Jensen, W. Odenbach and O. Schieder (Eds.), Proc. Intl. Symp. Genetic Manipulation in Plant Breeding, EUCARPIA, September 8–13, 1985, Berlin, pp. 351–353.

    Google Scholar 

  • Symko, S., 1969. Haploid barley from crosses of Hordeum bulbosum (2 x) x H. vulgare (2 x). Can. J. Genet. Cytol. 11: 602–608.

    Google Scholar 

  • Tao, Z.R., M.S. Liu and Z.C. Zhu, 1985. In vitro production of haploid plantlets from unpollinated ovaries of potato. Hereditas 7: 5.

    Google Scholar 

  • Tao, Z.R., M.S. Liu and Z.C. Zhu, 1988. Induction of dihaploid plantlets from unpollinated ovaries of potato in vitro. Acta Genet. Sin. 15: 329–334.

    Google Scholar 

  • Tian, H.Q. and H.Y. Yang, 1989. Haploid embryogeny and plant regeneration in unpollinated ovary culture of Allium tuberosum. Acta Biol. Exp. Sin. 22: 139–147.

    Google Scholar 

  • Tulecke, W., 1964. A haploid tissue culture from the female gametophyte of Ginkgo biloba. Nature 203: 94–95.

    Article  Google Scholar 

  • Uchimiya, H., T. Kameya and N. Takahashi, 1971. In vitro culture of unfertilized ovules in Solanum melongena and ovaries in Zea mays. Jpn. J. Breed. 21: 247–250.

    Google Scholar 

  • Van Geyt, J. and M. Jacobs, 1986. Induction of haploids of sugarbeet (Beta vulgaris L.) by means of androgenesis and gynogenesis. Bull. Soc. Bot. France. Act. Bot. 133: 4, 83.

    Google Scholar 

  • Van Geyt, J., G.J. Speckmann Jr., K. D’Halluin and M. Jacobs, 1987. In vitro induction of haploid plants from unpollinated ovules and ovaries of the sugarbeet (Beta vulgaris L.). Theor. Appl. Genet. 73: 920–925.

    Google Scholar 

  • Wagner, V.T., C.S. Yang, E. Matthys-Rochon and C. Dumas, 1989. Observation on the isolated embryo sac of Zea mays L. Plant Sci. 59: 127–132.

    Article  Google Scholar 

  • Wang, Q.Z., Z.X. Wang and X.H. Zhang, 1981. Initial success in the culture of monoploid plants from the unpollinated ovary of Robinia pseudoacacia. Forest Sci. Technol. Linye Keji Tongxun 7: 5–6.

    Google Scholar 

  • Wang, S., A. Hang and T. Tsuchiya, 1991. Chromosome studies of callus tissues and regenerated plants from an unfertilized ovule culture of sugarbeet (Beta vulgaris L.). J. Genet. Breed. 45: 161–168.

    Google Scholar 

  • Wu, B.J. and K.C. Cheng, 1982. Cytological and embryological studies on haploid plant produc- tion from cultured unpollinated ovaries of Nicotiana tabacum. Acta Bot. Sin. 2: 125–129.

    CAS  Google Scholar 

  • Yan, H., Y. Wu, X.M. Chen, Z.Y. Wie, C. Zhou and H.Y. Yang, 1985. Microscopic observations on embryoid formation in cultured unfertilized ovules of Helianthus annuus. Acta Bot. Sin. 27: 13–18.

    Google Scholar 

  • Yang, H., C. Zhou, D. Cai, H. Yan, Y. Wu and X. Chen, 1986. In vitro culture of unfertilized ovules in Helianthus annuus L. In: H. Hu and H. Yang (Eds.), Haploids of Higher Plants In Vitro, pp. 183–191. Springer-Verlag, Berlin.

    Google Scholar 

  • Zhang, Y.X., Y. Lespinasse and E. Chevreau, 1988. Obtention de plantes haploïdes de pommier (Malus x domestica Borlch.) issues de parthénogénèse induite in situ par du pollen irradié et culture in vitro des pépins immatures. C.R. Acad. Sci. Paris Sér. III. 307: 451–457.

    Google Scholar 

  • Zhou, C. and H.Y. Yang, 1980. In vitro induction of haploid plantlets from unpollinated young ovaries of Oryza sativa. Acta Genet. Sin. 7: 287–288.

    Google Scholar 

  • Zhou, C. and H.Y. Yang, 1981. Induction of haploid rice plantlets by ovary culture. Plant Sci. Lett. 20: 231–237.

    Google Scholar 

  • Zhou, C. and H.Y. Yang, 1986. Isolation of embryo sacs by enzymatic maceration and its potential in haploid study. In: H. Hu and H. Yang (Eds.), Haploids of Higher Plants In Vitro, pp. 192–203. Springer-Verlag, Berlin.

    Google Scholar 

  • Zhou, C., H. Yang, H. Tian, Z. Liu and H. Yan, 1986. In vitro culture of unpollinated ovaries in Oryza sativa L. In: H. Hu and H. Yang (Eds.), Haploids of Higher Plants In Vitro, pp. 165–181. Springer-Verlag, Berlin/Heidelberg/New York.

    Google Scholar 

  • Zhu, Z.C. and H.S. Wu, 1979. Production of haploid plantlets from unpollinated ovaries of Triticum aestivum and Nicotiana tabacum. Acta Genet. Sin. 6: 181–183.

    Google Scholar 

  • Zhu, Z.C., H.S. Wu, Q.K. An and Z.Y. Liu, 1981. Induction of haploid plantlets from unpollinated ovaries of Triticum aestivum cultured in vitro. Acta Genet. Sin. 8: 386–390.

    Google Scholar 

  • Zhu, Z.C., H.S. Wu, Q.K. An and Z.Y. Liu, 1983a. Induction of haploid plantlets from unpollinated wheat ovaries. In: Cell and Tissue Culture Techniques for Cereal Crop Improvement, p. 439. Science Press, Beijing.

    Google Scholar 

  • Zhu, Z.C., H.S. Wu, Z.Y. Liu and Q.K. An, 1983b. Production of haploid plantlets from unpollinated ovaries. In: Cell and Tissue Culture Techniques for Cereal Crop Improvement, p. 441. Science Press, Beijing.

    Google Scholar 

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Authors
  1. E. R. Joachim Keller
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  2. Larissa Korzun
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Editors and Affiliations

  1. Plant Production Department, University of Helsinki, Helsinki, Finland

    S. Mohan Jain

  2. School of Life Science, Jawaharlal Nehru University, New Delhi, India

    S. K. Sopory

  3. Department of Horticulture, Virginia Polytechnic Institute and State University, Blacksburg, Virginia, USA

    R. E. Veilleux

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Keller, E.R.J., Korzun, L. (1996). Ovary and ovule culture for haploid production. In: Jain, S.M., Sopory, S.K., Veilleux, R.E. (eds) In Vitro Haploid Production in Higher Plants. Current Plant Science and Biotechnology in Agriculture, vol 23. Springer, Dordrecht. https://doi.org/10.1007/978-94-017-1860-8_13

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  • DOI: https://doi.org/10.1007/978-94-017-1860-8_13

  • Publisher Name: Springer, Dordrecht

  • Print ISBN: 978-90-481-4579-9

  • Online ISBN: 978-94-017-1860-8

  • eBook Packages: Springer Book Archive

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