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In Vitro Haploid Production in Higher Plants pp 1–10Cite as

The discovery of anther culture technique for the production of haploids

A personal reflection

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Part of the book series: Current Plant Science and Biotechnology in Agriculture ((PSBA,volume 23))

Abstract

The discovery of haploid production by anther culture came partly by accident, and partly by the coincidence of some unusual circumstances. It is my hope that in these memorabilia the readers will find something of interest as most people do not know of the events that led to this discovery.

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References

  • Bajaj, Y.P.S. (Ed.), 1990. Biotechnology in Agriculture and Forestry, Vol. 12: Haploids in Crop Improvement. Part I. Springer-Verlag, Berlin.

    Google Scholar 

  • Blakeslee, A.F., J. Belling, M.E. Farnham and A.D. Bergner, 1922. A haploid mutant in the Jimson weed, Datura stramonium. Science 55: 646–647.

    Article  PubMed  CAS  Google Scholar 

  • Bourgin, J.P. and J.P. Nitsch, 1967. Obtention de Nicotiana haploids à partir d’étamines cultivées in vitro. Ann. Physiol. Veg. 9: 377–382.

    Google Scholar 

  • Campos, F.F. and D.T. Morgan Jr., 1958. Haploid pepper from a sperm. J. Hered. 49: 134137.

    Google Scholar 

  • Chase, S.S., 1947. Techniques for isolating monoploid maize plants. Amer. J. Bot. 34: 582. Clausen, R.E. and W.E. Lammerts, 1929. Interspecific hybridisation in Nicotiana. X. Haploid and diploid mcrogony. Amer. Nat. 43: 279–282.

    Google Scholar 

  • Gerassimova, H., 1939. Experimentelle erhaltene haploide Pflanze von Crepis tectorum. Planta 25: 696–702.

    Article  Google Scholar 

  • Gregory, W.C., 1940. Experimental studies on the cultivation of excised anthers in nutrient solution. Amer. J. Bot. 27: 687–692.

    Google Scholar 

  • Guha, S., R.D. Iyer, N. Gupta and M.S. Swaminathan, 1970. Totipotency of gametic cells and the production of haploids in rice. Curr. Sci. 39: 174–176.

    Google Scholar 

  • Guha, S. and S.C. Maheshwari, 1964. In vitro production of embryos from anthers of Datura. Nature 204: 497.

    Google Scholar 

  • Guha, S. and S.C. Maheshwari, 1966. Cell division and differentiation of embryos in the pollen grains of Datura in vitro. Nature 212: 97–98.

    Article  Google Scholar 

  • Guha, S. and S.C. Maheshwari, 1967. Development of embryoids from pollen grains of Datura in vitro. Phytomorphology 17: 454–461. ( Invited article in P. Maheshwari Memorial Issue. )

    Google Scholar 

  • Hermsen, J.G.Th., 1974. Summation: Haploids in plant breeding. In: K.J. Kasha (Ed.), Haploids in Higher Plants: Advances and Potential, pp. 281–285. Proceedings of the First International Symposium, June 10–14, 1974, University of Guelph, Guelph.

    Google Scholar 

  • Hu, H. (Ed.), 1978. Proceedings of Sino-Australian Symposium on Plant Tissue Culture. Science Press, Peking.

    Google Scholar 

  • Hu, H. and H. Yang (Eds.), 1986. Haploids of Higher Plants In Vitro. China Academic Publishers, Beijing/Springer-Verlag, Berlin.

    Google Scholar 

  • Johannsen, W., 1903. Über Erblichkeit in Populationen und in reinen Linien. Gustav Fischer, Jena.

    Google Scholar 

  • Johannsen, W., 1909. Elemente der exacten Erblichkeitslehre. Gustav Fischer, Jena.

    Google Scholar 

  • Kasha, K.J. (Ed.), 1974. Haploids in Higher Plants: Advances and Potential. Proceedings of the First International Symposium, June 10–14, 1974, University of Guelph, Guelph.

    Google Scholar 

  • Katayama, Y. and M. Nei, 1964. Studies on the haploidy in higher plants. Rep. Lab. Plant Breed., Fac. Agr., Miyazaki Univ., No. 2: 1–78 (in Japanese).

    Google Scholar 

  • Kihara, H. and Y. Tsunewaki, 1962. Use of alien cytoplasm as a new method of producing haploids. Jpn. J. Genet. 37: 310–313.

    Google Scholar 

  • Kimber, G. and R. Riley, 1963. Haploid angiosperms. Bot. Rev. 29: 480–531.

    Google Scholar 

  • Konar, R.N., 1963. A haploid tissue from the pollen of Ephedra foliata Boiss. Phytomorphology 13: 170–174.

    Google Scholar 

  • Konar, R.N. and K. Nataraja, 1965. Production of embryoids from the anther of Ranunculus sceleratus L. Phytomorphology 15: 245.

    Google Scholar 

  • Kostoff, D., 1929. An androgenic Nicotiana haploid. Zeit. Zellforschg. 9: 640–642.

    Google Scholar 

  • Lacadena, J.-R., 1974. Spontaneous and induced parthenogenesis and androgenesis. In: K.J. Kasha (Ed.), Haploids in Higher Plants: Advances and Potential, pp. 13–32. Proceedings of the First International Symposium, June 10–14, University of Guelph, Guelph.

    Google Scholar 

  • Linskens, H.F., 1956. Physiologische Untersuchungen zur Reifeteilung. I. Mitteilung. Über die Änderung einiger physiologischer Zustandsgrossen während der Pollenmeiose und Pollenentwicklung von Lilium henryi. Ber. Deutsch. Bot. Ges. 69: 353–360.

    Google Scholar 

  • Maheshwari, N., 1958. In vitro culture of excised ovules of Papaver somniferum. Science 127: 342.

    Google Scholar 

  • Maheshwari, P., 1950. An Introduction to the Embryology of Angiosperms. McGraw-Hill Book Co., New York/Toronto/London.

    Google Scholar 

  • Maheshwari, P. and N.S. Rangaswamy, 1963. Plant tissue and organ culture from the viewpoint of an embryologist. In: Plant Tissue and Organ Culture–A symposium, pp. 390–420. International Society of Plant Morphologists, Delhi.

    Google Scholar 

  • Maheshwari, S.C., 1982. My odyssey of twenty-five years of research: From morphology towards molecular botany. J. Indian Bot. Soc. 61: 1–8.

    Google Scholar 

  • Maheshwari, S.C., A. Rashid and A.K. Tyagi, 1982. Haploids from pollen grains - retrospect and prospect. Amer. J. Bot. 69: 865–879 (special paper).

    Google Scholar 

  • Maheshwari, S.C., A.K. Tyagi, K. Malhotra and S.K. Sopory, 1980. Induction of haploidy from pollen grains in angiosperms - the current status. Theor. Appt Genet. 58: 193–206.

    Google Scholar 

  • Malhotra, K. and S.C. Maheshwari, 1978. Enhancement by cold treatment of pollen embryoid development in Petunia hybrida. Z. Pflanzenphysiol. 85: 177–180.

    Google Scholar 

  • Muller, H.J., 1927. Artificial transmutation of the genes. Science 66: 84–87.

    Article  PubMed  CAS  Google Scholar 

  • Nakata, K. and M. Tanaka, 1968. Differentiation of embryoids from developing germ cells in anther culture of tobacco. Jpn. J. Genet. 43: 65–71.

    Google Scholar 

  • Nei, M., 1963. Efficiency of haploid method of plant breeding. Heredity 18: 95–100.

    Article  Google Scholar 

  • Niizeki, H. and K. Oono, 1968. Induction of haploid rice plant from anther culture. Proc. Jpn. Acad. 44: 554–557.

    Google Scholar 

  • Nitsch, C., 1974a. La culture de pollen isolé sur milieu synthétique. C.R. Acad. Sci. Paris 278D: 1031–1034.

    Google Scholar 

  • Nitsch, C., 1974b. Pollen culture - a new technique for mass production of haploid and homozygous plants. In: K.J. Kasha (Ed.), Haploids in Higher Plants: Advances and Potential, pp. 123–135. Proceedings of the first international symposium, June 10–14, 1974. University of Guelph, Guelph.

    Google Scholar 

  • Nitsch, C. and B. Norreel, 1973. Effect d’un choc thermique sur la pouvoir embryogène du pollen de Datura innoxia cultivé dans l’anthère ou isolé de l’anthère. C.R. Acad. Sci. Paris 276D: 303–306.

    Google Scholar 

  • Nitsch, J.P. and C. Nitsch, 1969. Haploid plants from pollen grains. Science 163: 85–87. Nitzsche, W. and G. Wenzel, 1977. Haploids in Plant Breeding. Vol. 8. In: Advances in Plant Breeding. Verlag Paul Parey, Berlin.

    Google Scholar 

  • Otten, L., H. De Greve, J.P. Hernalsteens, M. Van Montagu, O. Schieder, J. Straub and J. Schell, 1981. Mendelian transmission of genes introduced into plants by the Ti plasmid of Agrobacterium tumefaciens. Mol. Gen. Gen. 183: 209–213.

    Google Scholar 

  • Pechan, P.M. and J. Schell, 1990. Molecular changes associated with commitment phase of microspore embryogenesis. In: H.J.J. Nijkamp et al. (Eds.), Progress in Plant Cellular and Molecular Biology, pp. 213–221. Kluwer Academic Publishers, Dordrecht.

    Google Scholar 

  • Raghavan, V., 1990. From microspore to embryoid: Faces of the Angiosperm pollen grain. In: H.J.J. Nijkamp et al. (Eds.), Progress in Plant Cellular and Molecular Biology, pp. 407–409. Kluwer Academic Publishers, Dordrecht.

    Google Scholar 

  • Rangan, T.S., 1984. Culture of ovules. In: Cell Culture and Somatic Cell Genetics of Plants, pp. 227–230. Vasil, I.K. (Ed.), Vol. 1. Academic Press, New York.

    Google Scholar 

  • Reynolds, T.L. and S.L. Kitto, 1992. Identification of embryoid-abundant genes that are temporally expressed during pollen embryogenesis in wheat anther cultures. Plant Physiol. 100: 1744–1750. ~

    Google Scholar 

  • Sopory, S.K. and S.C. Maheshwari, 1973. Similar effects of cytokinins and EDDHA in production of haploid plants. Z. Pflanaenphysiol. 69: 97–99.

    CAS  Google Scholar 

  • Sopory, S.K. and S.C. Maheshwari, 1976a. Development of pollen embryoids in Datura innoxia. I. General observations and effect of physical factors. J. Exp. Bot. 27: 49–57.

    Google Scholar 

  • Sopory, S.K. and S.C. Maheshwari, 1976b. Development of pollen embryoids in Datura innoxia. Il. Effect of growth hormones. J. Exp. Bot. 27: 58–68.

    Google Scholar 

  • Sparrow, A.H., V. Pond and S. Kojan, 1955. Microsporogenesis in excised anthers of Trillium erectum grown on sterile media. Amer. J. Bot. 42: 384–394.

    Google Scholar 

  • Sunderland, N., 1974. Anther culture as a means of haploid induction. In: K.J. Kasha (Ed.), Haploids in Higher Plants: Advances and Potential, pp. 91–122. Proceedings of the First International Symposium, June 10–14, 1974. University of Guelph, Guelph.

    Google Scholar 

  • Taylor, J.H., 1950. The duration of differentiation in excised anthers. Amer. J. Bot. 37: 137143.

    Google Scholar 

  • Tulecke, W.R., 1953. A tissue derived from the pollen of Ginkgo biloba. Science 117: 599600.

    Google Scholar 

  • Tulecke, W.R., 1959. The pollen cultures of C.D. LaRue: A tissue from the pollen of Taxus. Bull. Torrey Bot. Club. 86: 283–289.

    Google Scholar 

  • Tulecke, W.R. and N. Sehgal, 1963. Cell proliferation from the pollen of Torreya nucifera. Contrib. Boyce Thompson Inst. 22: 153–163.

    Google Scholar 

  • Tyagi, A.K., A. Rashid and S.C. Maheshwari, 1979. High frequency production of embryoids in Datura innoxia from isolated pollen grains by combined cold treatment and serial culture of anthers in liquid medium. Protoplasma 99: 11–17.

    Article  Google Scholar 

  • Tyagi, A.K., A. Rashid and S.C. Maheshwari, 1980. Enhancement of pollen embryo formation in Datura innoxia by charcoal. Physiol. Plant. 49: 296–298.

    Google Scholar 

  • Tyagi, A.K., A. Rashid and S.C. Maheshwari, 1981. Promotive effect of polyvinylpolypyrrolidone on pollen embryogenesis in Datura innoxia. Physiol. Plant. 53: 405–406.

    Google Scholar 

  • Vasil, I.K., 1960. Physiology of anthers. In: P. Maheshwari et al. (Eds.), Proceedings Summer School of Botany, Darjeeling. Ministry of Scientific Research and Cultural Affairs, Government of India, New Delhi.

    Google Scholar 

  • Veilleux, R.E., 1994. Development of new cultivars via anther culture. HortScience 29: 1238 1241.

    Google Scholar 

  • Wray, J.L., 1986. The molecular genetics of higher plant nitrate assimilation. In: A.D. Blonstein and P.J. King (Eds.), A Genetic Approach to Plant Biochemistry. Springer-Verlag, Berlin.

    Google Scholar 

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Authors
  1. S. C. Maheshwari
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Editors and Affiliations

  1. Plant Production Department, University of Helsinki, Helsinki, Finland

    S. Mohan Jain

  2. School of Life Science, Jawaharlal Nehru University, New Delhi, India

    S. K. Sopory

  3. Department of Horticulture, Virginia Polytechnic Institute and State University, Blacksburg, Virginia, USA

    R. E. Veilleux

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© 1996 Springer Science+Business Media Dordrecht

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Maheshwari, S.C. (1996). The discovery of anther culture technique for the production of haploids. In: Jain, S.M., Sopory, S.K., Veilleux, R.E. (eds) In Vitro Haploid Production in Higher Plants. Current Plant Science and Biotechnology in Agriculture, vol 23. Springer, Dordrecht. https://doi.org/10.1007/978-94-017-1860-8_1

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  • DOI: https://doi.org/10.1007/978-94-017-1860-8_1

  • Publisher Name: Springer, Dordrecht

  • Print ISBN: 978-90-481-4579-9

  • Online ISBN: 978-94-017-1860-8

  • eBook Packages: Springer Book Archive

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