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Utilization of Brassica haploids

  • Chapter
In Vitro Haploid Production in Higher Plants

Part of the book series: Current Plant Science and Biotechnology in Agriculture ((PSBA,volume 25))

Abstract

Haploid embryos can be produced at high frequency by anther and microspore culture of many Brassica species and commercial cultivars (Keller et al.,1984, 1987; Lichter, 1989; Swanson, 1990; Arnison and Keller, 1990; Duijs et al., 1992; Palmer et al.,1994). The procedures for such production are routine although there are still significant genetic limitations to recovery of haploids from some genotypes. The development of procedures for efficient recovery of haploids was driven principally by their potential usefulness in the development of homozygous lines for breeding programs (Wenzel et al.,1977). The high regenerative potential of haploid microspores, especially when cultured in isolation from the anther tissues, provides an ideal system for mutagenesis and the recovery of mutants. In addition, the recovery of doubled haploids through chromosome doubling techniques ensures that mutant genes are fixed in the homozygous state.

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Authors
  1. Constantine E. Palmer
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  2. Wilfred A. Keller
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  3. Paul G. Arnison
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Editor information

Editors and Affiliations

  1. Plant Production Department, University of Helsinki, Helsinki, Finland

    S. Mohan Jain

  2. School of Life Science, Jawaharlal Nehru University, New Delhi, India

    S. K. Sopory

  3. Department of Horticulture, Virginia Polytechnic Institute and State University, College of Agriculture and Life Science, Blacksburg, 24061-0383, Virginia, USA

    R. E. Veilleux

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© 1996 Springer Science+Business Media Dordrecht

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Palmer, C.E., Keller, W.A., Arnison, P.G. (1996). Utilization of Brassica haploids. In: Jain, S.M., Sopory, S.K., Veilleux, R.E. (eds) In Vitro Haploid Production in Higher Plants. Current Plant Science and Biotechnology in Agriculture, vol 25. Springer, Dordrecht. https://doi.org/10.1007/978-94-017-1858-5_10

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  • DOI: https://doi.org/10.1007/978-94-017-1858-5_10

  • Publisher Name: Springer, Dordrecht

  • Print ISBN: 978-90-481-4581-2

  • Online ISBN: 978-94-017-1858-5

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