Abstract
N2-fixation is sensitive to limitation in the availability of newly synthesised carbohydrates for the nodules. We decided to explore the response of the D. trinervis — Frankia symbiosis to a transient decrease in carbohydrate supply to nodules. Feedback inhibition of nodulation as well as nodule growth was not released by a 6-day dark stress in D. trinervis nodulated plants. However, nitrogen fixation and assimilation were affected by the imposed stress. Nitrogenase activity was totally inhibited after 4 days of darkness although high levels of nitrogenase components were still detected at this time. Degradation of FeMo and Fe nitrogenase subunits — both at similar rates — was observed after 6 days of dark stress, revealing the need for inactivation to precede enhancement of protein turnover. Glutamine synthetase (GS), malate dehydrogenase (MDH) and asparagine synthetase (AS) polypeptides were also degraded during the dark stress, although at a lower rate than nitrogenase. ARA and nitrogenase were totally recovered 8 days after resuming normal illumination. It seems that current nitrogenase activity and ammonium assimilation are not, or are only weakly linked with the feedback control of nodulation in D. trinervis. These observations give support to the persistence of an autoregulatory signal in mature nodules that is not sensitive to transient shortages of carbon supply and sustains the inhibition of nodulation in the transient absence of N2 fixation.
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Abbreviations
- AS:
-
asparagine synthetase
- GS:
-
glutamine synthetase
- MDH:
-
malate dehydrogenase
References
Akkermans A D L, Huss-Danell K and Roloefsen W 1981 Enzymes of the tricarboxilic acid cycle and the malate-aspartate shuttle in the N2-fixing endophyte of Alms glutinosa. Physiol. Plant. 53, 289–294.
Blake M S, Johnston K H, Russell-Jones G J and Gotschlich E C 1984 A rapid, sensitive method for detection of alkaline phosphatase conjugated anti-antibody on western blots. Anal. Biochem. 136, 175–79.
BRENDA. The comprehensive enzyme information system. www. brenda.uni-koeln.de
Burette W N 1981 `Western blotting’: Electrophoretic transfer of proteins from sodium dodccyl sulfate-polyacrylamide gels to unmodified nitrocellulose and radiographic detection with antibody and radioidinated Protein A. Anal. Biochcm. 112, 195–203.
Chaia E 1998 Isolation of an effective strain of Frankia from nodules of Discaria trinervis (Rhamanceae). Plant Soil 205, 99–102.
Egli M A, Griffith S M, Miller S S, Anderson M P and Vance C P 1989 Nitrogen assimilating enzyme activities and enzyme protein during development and senescence of effective and plant gene-controlled ineffective alfalfa nodules. Plant. Physiol. 91, 898–904.
Heim I, Hartwig U A and Nösberger J 1993 Current nitrogen fixation is involved in the regulation of nitrogenase activity in white clover (Trifolium repens L.). Plant Physiol. 103, 1009–1014.
Huss-Danell K 1978 Nitrogenase activity measurements in intact plants of Alnus incana. Physiol. Plant., 43, 342–348.
Huss-Danell K 1997 Actinorhizal symbioses and their N’ fixation New Phytol. 136, 375–405.
Laemmli U K 1970 Cleavage of structural proteins during the assembly of the head of the bacteriophage T4. Nature 227, 680–685.
Lundquist PO and Huss-Danell K 199la Nitrogenase activity and amounts of nitrogenase proteins in a Frankia-Alnus incana symbiosis subjected to darkness. Plant Physiol. 95, 808–813.
Lundquist PO and Huss-Dancll K 1991b Response of nitrogenase to altered carbon supply in a Frankia-Aldus incana symbiosis. Physiol. Plant. 83, 331–338.
Murry M A, Hallenbeck P C, Esteva D and Benemann J R 1983 Nitrogenase inactivation by oxygen and enzyme turnover in Anabaena cvlindrica. Can. J. Microbiol. 29, 1286–1294.
Nittayajarn A and Baker DD 1989 Methods for the quantification of Frankia cell biomass. Plant Soil 1 18, 199–204.
Parsons R, Stanforth A, Raven J A and Sprent J 1993 Nodule growth and activity may be regulated by a feedback mechanism involving phloem nitrogen. Plant Cell Environ. 16, 125–136.
Romanov V I, Gordon A J, Minchin F R, Witty J F, Skot L, James C L and Tikhonovich 1 A 1998 Physiological and biochemical characteristics of FN I, a `fixation impaired’ mutant of pea (Pisum sativum L.). J. Exp. Bot. 49, 1789–1796.
Smith P K, Krohn R 1, Hermanson G T, Mallia A K, Gartner F H, Provenzano M D, Fujimoto E K, Goeke NM, Olson B J and Kienk D C 1985 Measurement of protein using bicinchoninic acid. Anal. Biochem. 150, 76–85.
Swensen S M and Mullin B C 1997 Phylogenetic relationships among actinorhizal plants. The impact of molecular systematics and implications for the evolution of actinorhizal symbiosis. Physiol. Plant. 99, 565–573.
Valverde C and Wall L G I 999a Time course of nodule development in Discaria trinervis (Rhamnaceae)-Frankia symbiosis. New Phytol. 141, 345–354.
Valverde C and Wall L G 1999b Regulation of nodulation in Dis-caria trinervis (Rhamnaceae)-Frankia symbiosis. Can. J. Bot. 77. 1302–1310.
Valverde C 2000 `Regulaciôn de la nodulacion radicular en la simbiosis Discaria trinenis — Frankia’. Ph. D. Thesis, Universidad Nacional de La Plata, Argentina.
Valverde C. Wall L G and Huss-Danell K 2000 Regulation of modulation and nodule mass relative to nitrogenase activity and nitrogen demand in seedlings of Discaria trinervis ( Rhamnaceae ). Symbiosis 28, 49–62.
Valverde C and Wall L G 2003 Ammonium assimilation in Discaria trinervis root nodules. Regulation of enzyme activities and protein levels by the availability of macronutrients (N, P and C). Plant Soil 254, 139–153.
Vance C P, Johnson L E B, Halvorsen A M, IIeichel G H and Barnes D K 1980 Histological and ultrastructural observations of Nledicago saliva root nodule senescence after foliage removal. Can. J. Hot. 58, 295–309.
Vance C P 2000 Amide biosynthesis in root nodules of temperate legumes. In Prokaryotic Nitrogen Fixation: A Model System for Analysis of a Biological Process. Ed. E W Triplett. pp. 589–607. Horizon Scientific Press, Wymondham, UK.
Vikman P -A, Lundquist P -O and Huss-Danell K 1990 Respiratory capacity, nitrogenase activity and structural changes of Frankia in symbiosis with Alnus incana, in response to prolonged darkness. Planta 182, 617–625.
Wall L G 2000 The actinorhizal symbiosis. J. Plant Growth Reg. 19, 167–182.
Wall I,G and Huss-Danell K 1996 Laactividad lijadora de nitrogeno como factor de regulacibn de la nodulaci6n en la simbiosis Aldus incana — Frankia. In Proceedings of the XXI Meeting of the Argentinean Society of Plant Physiology (Mendoza, Argentina, 20–22 March 1996 ). pp. 450–451.
Wheeler C T 1971 The causation of the diurnal changes in nitrogen fixation in the nodules of Alnus glutinosa New Phytol. 70,487— 495.
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Valverde, C., Wall, L.G. (2003). The regulation of nodulation, nitrogen fixation and ammonium assimilation under a carbohydrate shortage stress in the Discaria trinervis-Frankia symbiosis. In: Normand, P., Dawson, J.O., Pawlowski, K. (eds) Frankia Symbiosis. Developments in Plant and Soil Sciences, vol 100. Springer, Dordrecht. https://doi.org/10.1007/978-94-017-1601-7_17
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DOI: https://doi.org/10.1007/978-94-017-1601-7_17
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