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Immobilised Enzymes: Characterisation and Functional Meaning in Soil Amendments of Organic Wastes

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Environmental Monitoring and Biodiagnostics of Hazardous Contaminants
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Abstract

This paper reports on the study of effects on total and immobilised enzyme activities (urease and phosphatase) on amendment of organic waste to arid soil. The organic materials contained considerable values of total urease and phosphatase activity. The fresh organic materials contained the highest values for both total enzymatic activities. The addition of organic waste to soil increased both total enzymatic activities of soil and after 360 days, the soil treated showed values above those of the control. Enzymes immobilised in clay-enzyme or humus-enzyme complexes may have an important role on the microbial nutrition. These enzymes are very stable and are resistant to degradation. The organic wastes form enzyme-humus complex which were higher in the fresh wastes than in the compost, however, the specific enzymatic activities (enzymatic activity by unit of carbon) were similar. The immobilised urease activity was greater in the soil with organic amendments than in the control. At the beginning of the incubation period, the immobilised urease activity was significantly higher in the soil amended with fresh organic wastes than with compost. However, this activity decreased with incubation whilst the compost immobilised urease activity increased with time. The effect of organic amendments on immobilised phosphatase activity was similar to immobilised urease but less significant.

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Pascual, J.A., Hernandez, T., Garcia, C., Lynch, J. (2001). Immobilised Enzymes: Characterisation and Functional Meaning in Soil Amendments of Organic Wastes. In: Healy, M., Wise, D.L., Moo-Young, M. (eds) Environmental Monitoring and Biodiagnostics of Hazardous Contaminants. Springer, Dordrecht. https://doi.org/10.1007/978-94-017-1445-7_17

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  • DOI: https://doi.org/10.1007/978-94-017-1445-7_17

  • Publisher Name: Springer, Dordrecht

  • Print ISBN: 978-90-481-5674-0

  • Online ISBN: 978-94-017-1445-7

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