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Entomopathogenic Bacteria: from Laboratory to Field Application pp 275–295Cite as

Is Bacillus thuringiensis standardisation still possible?

Update and improvement of Bt titration over 20 years

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Abstract

The aim of this review is to clarify the situation surrounding titration of bacterial formulations against Bacillus reference standards and to discuss the importance of standard procedures. Secondly, with the presence of numerous B. thuringiensis Cry toxins, we examine a possible remedy for the absence of classical reference standards. We propose a new way of titrating Bt products combining insect based bioassays and quantitative determination of toxin protein with biochemical methods. Further, we suggest improvement in the standard protocols used for bioassays and the statistical methods applied.

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Author information

Authors and Affiliations

  1. Intelligent Insect Control, 80 rue Paul Ramart, Montpellier, 34070, France

    Ole Skovmand

  2. Laboratoire des Bactéries et Champignons Entomopathogènes, Institut Pasteur, Paris, France

    Isabelle Thiéry

  3. Benzon Research, Carlisle, Pennsylvania, USA

    Gary Benzon

Authors
  1. Ole Skovmand
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  2. Isabelle Thiéry
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  3. Gary Benzon
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Editor information

Editors and Affiliations

  1. Institut Pasteur, Paris, France

    Jean-François Charles , Armelle Delécluse  & Christina Nielsen-Le Roux ,  & 

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Skovmand, O., Thiéry, I., Benzon, G. (2000). Is Bacillus thuringiensis standardisation still possible?. In: Charles, JF., Delécluse, A., Roux, C.NL. (eds) Entomopathogenic Bacteria: from Laboratory to Field Application. Springer, Dordrecht. https://doi.org/10.1007/978-94-017-1429-7_15

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  • DOI: https://doi.org/10.1007/978-94-017-1429-7_15

  • Publisher Name: Springer, Dordrecht

  • Print ISBN: 978-90-481-5542-2

  • Online ISBN: 978-94-017-1429-7

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