Abstract
The use of haploid systems for mutant induction and selection has been listed among the most important applications of haploid technologies, since their development (Kasha, 1974). Haploid tissue can facilitate the generation of genetic variation and its identification. The haploid system provides several advantages for the application of mutation techniques in plant breeding and germplasm enhancement, however under the condition that an efficient methodology of doubled haploid production is available for a particular species. Many of these advantages arise from the fact that genotypes and genetic segregation ratios in DH populations are equivalent to those found in gametes. Some of the benefits of applying DH systems for induction and selection of mutants are:
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possibility to screen for both recessive and dominant mutants in the first generation after mutagenic treatment
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immediate fixation of mutated genotypes, which saves time in the production of pure mutant lines
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increased selection efficiency of desired mutants due to the gametic versus zygotic segregation ratios (1:1 vs 3:1, respectively) and the lack of chimerism
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possibility of applying in vitro selection methods at the haploid or doubled haploid level.
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Szarejko, I. (2003). Doubled haploid mutant production. In: Maluszynski, M., Kasha, K.J., Forster, B.P., Szarejko, I. (eds) Doubled Haploid Production in Crop Plants. Springer, Dordrecht. https://doi.org/10.1007/978-94-017-1293-4_48
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