Abstract
Forty-four protocols of doubled haploid production (DH) are presented in this Manual. They are related to at least 33 plant species, not counting protocols on production of DH from interspecific hybrids. The protocols provide procedures for production of DH in such major crops as wheat, maize, barley, rapeseed or potato and also in other crop species where this technology is more advanced, or in other words, where a relatively high frequency of doubled haploids was obtained. However, there have been several approaches to develop doubled haploids in many crops or plant species. We are presenting below the list of publications related to DH production in 226 additional plant species. This list does not include protocols of plant species published in Chapter 2 of this Manual. This means that efforts have been undertaken to develop doubled haploids in more than 250 plant species as the presented list was built on the basis of our own collection of related publications and as a result of searching in publicly available databases. It was impossible to cite all published information. We limited references to those which carried protocols or more recent information on DH production. In the cited papers, presented protocols did not always lead to the regeneration of haploid or doubled haploid plants. However, even negative results can be very helpful for someone who is initiating this work. We hope that publication of this list will help in further application of DH technology in crop improvement and basic research.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
Abou-Mandour, A.A., S. Fischer and F.C. Czygan, 1979. Regeneration of intact plants from haploid and diploid callus cells of Catharanthus roseus. Z.Pflanzenphysiol. 91: 83–88.
Abraham, A., P.N. Krishnan and S. Seeni, 1995. Induction of androgenesis, callus formation and root differentiation in anther culture of cassava (Manihot esculenta Crantz). Indian J.Exp.Biol. 33 (3): 186–189.
Adda, S., T.P. Reddy and P.B. Kavi Kishor, 1994. Androclonal variation in niger (Guizotia abyssinica Cass). Euphytica 79: 59–64.
Ahmin, M. and J. Vieth, 1986. Regeneration of haploid plants of Gerbera jamesonii by ovules cultivated in vitro. Can.J.Bot. 64: 2355–2357.
Ali, M.A. and J.K. Jones, 2000. Microspore culture in Corchorus olitorius: effect of growth regulators, teperature and sucrose on callus formation. Indian J.Exp.Biol. 38 (6): 593–597.
Altamura, M.M., A. Cersosimo, C. Majoli and M Crespan, 1992. Histological study of embryogenesis and organogenesis from anthers of Vitis rupestris du Lot cultured in vitro. Protoplasma 171 (34): 134–141.
Amos, J.A. and R.L. Scholl, 1978. Induction of haploid callus from anthers of four species of Arabidopsis. Z.Pflanzenphysiol. 90: 33–43.
Arrillaga, I., V. Lerma, P. Perez-Bermudez and J. Segura, 1995. Callus and somatic embryogenesis from cultured anthers of service tree (Sorbus domestica L.). HortSci. 30 (5): 1078–1079.
Arya, I.D. and N. Chandra, 1989. Organogenesis in anther-derived callus culture of cowpea (Vigna unguiculata (L.) Walp). Current Science 58 (5): 257–259.
Arzate-Fernandez, A.-M., T. Nakazaki and T. Tanisaka, 1998. Production of diploid and tetraploid interspecific hybrids between Lilium concolor and L. longiflorum by in vitro ovary slice culture. Plant Breed. 117: 479–484.
Assani, A., F. Bakry, F. Kerbellec, R. Haicour, G. Wenzel and B. Foroughi-Wehr, 2003. Production of haploids from anther culture of banana [Musa balbisiana (BB)]. Plant Cell Rep. 21: 511–516
Atanasov, A.I., M. Abadzhieva and V. Becheva, 1980. Study on organogenesis ability in somatic tissue cultures of Datura innoxia Mill. with haploid diploid and tetraploid origin. Genet.Sel. 13: 104–112.
Avetisov, V.A., 1976a. Production of haploids from anther cultures and isolated protoplast cultures of Arabidopsis thaliana (L.) Heynh. Sov.Genet. 12: 408–413.
Avetisov, V.A., 1976b. Production of haploids under in vitro culture of Arabidopsis thaliana (L.) Heynh. anthers and isolated protoplasts. Genetika 12: 17–25.
Babbar, S.B. and S.C. Gupta, 1984. Pathways in pollen sporophyte development in anther cultures of Datura metel and Petunia hybrida. Beitr.Biol.Pflanzen. 59: 475–488.
Babbar, S.B. and S.C. Gupta, 1986a. Effect of carbon source on Datura metel microspore embryogenesis and the growth of callus raised from microspore-derived embryos. Biochem.Physiol.Pflanz.BPP. 181: 331–338.
Babbar, S.B. and S.C. Gupta, 1986b. Induction of androgenesis and callus formation in in vitro cultured anthers of a myrtaceous fruit tree (Psidium guajava L.). Botanical Magazine 99 (1053): 75–83.
Babbar, S.B. and S.C. Gupta, 1986c. Putative role of ethylene in Datura metel microspore embryogenesis. Physiol.Plant. 68: 141–144
Babbar, S.B. and S.C. Gupta, 1990. Phasic requirement of coconut milk for Datura metel microspore embryogenesis. Phytomorphol. 40 (12): 53–57.
Badea, E., M. Iordan and A. Mihalea, 1985. Induction of androgenesis in anther culture of Digitalis lanata. Revue Roumaine de Biologie, Biologie Vegetale 30 (1): 63–71.
Badea, E. and P. Raicu, 1982. In vitro anther and pollen culture in haploids (n=12) and triploids (3n=36) of Datura innoxia Mill. Rev.Roum.Biol.Ser.Biol.Veg. 27: 163–165.
Badigannavar, A.M. and M.S. Kuruvinashetti, 1996. Callus induction and shoot bud formation from cultured anthers in sunflower (Helianthus annuus L.). Helia 19 (25): 39–46.
Bajaj, Y.P.S., 1978. Effect of super-low temperature on excised anthers and pollen-embryos of Atropa, Nicotiana and Petunia. Phytomorphol. 28 (2): 171–176.
Bajaj, Y.P.S. and M.S. Gill, 1989. Pollen-embryogenesis and chromosomal variation in anther culture of a diploid cotton (Gossypium arboreum L.). SABRAO J. 21 (1): 57–63.
Bajaj, Y.P.S. and M.S. Gill, 1997. In vitro induction of haploidy in cotton. In: In Vitro Haploid Production in Higher Plants. Jain,M.S., S.K.Sopory and R.E.Veilleux ( Eds.) Kluwer Academic Publishers, Dordrecht, pp. 165–174.
Bajaj, Y.P.S. and S.S. Gosal, 1987. Pollen embryogenesis and chromosomal variation in cultured anthers of chickpea. Int.Chickpea Newsletter 17: 12–13.
Bajaj, Y.P.S., A.K. Ram, K.S. Labana and H. Singh, 1981. Regeneration of genetically variable plants from the anther-derived callus of Arachis hypogaea and Arachis villosa. Plant Sci.Lett. 23 (1): 35–39.
Bajaj, Y.P.S., H. Singh and S.S. Gosal, 1980. Haploid embryogenesis in anther cultures of pigeon-pea (Cajanus cajan). Theor.Appl.Genet. 58: 157–159.
Baldursson, S., P. Krogstrup, J.V. Norgaard and S.B. Andersen, 1993a. Microspore embryogenesis in anther culture of three species of Populus and regeneration of dihaploid plants of Populus trichocarpa. Can.J.For.Res. 23 (9): 1821–1825.
Baldursson, S., J.V. Norgaard and P. Krogstrup, 1993b. Factors influencing haploid callus initiation and proliferation in megagametophyte cultures of Sitka spruce (Picea sitchensis). Silvae Genetica 42 (23): 79–86.
Ban, Y., T. Kokubu and Y. Miyaji, 1971. Production of haploid plant by anther-culture of Setaria italica. Kagoshima Univ.Fac.Agr.Bull. 21: 77–81.
Banks, D.J., 1988. Characteristics of a rare, monosomic peanut (Arachis hypogaea L.- -Leguminosae), with implications for haploidy discovery. Am.J.Bot. 97–98.
Bansal, U.K., G. Bassi, S.S. Gosal and D.R. Satija, 1991. Induction of pollen embryogenesis and cytological variability in Arachis hypogaea L. through anther culture. Indian J.Genet.Plant Breed. 51 (1): 125–129.
Bapat, V.A. and G. Wenzel, 1982. In vitro haploid plantlet induction in Physalis ixocarpa Brot. through microspore embryogenesis. Plant Cell Rep. 1: 154–156.
Baracaccia, G., C. Tomassini and M. Falcinelli, 1999. Further cytological evidence on the androgenesis pathway in pepper (Capsicum annuum L.). J.Genet.and Breed. 53 (3): 251–254.
Barcelo, P., A. Cabrera, C. Hagel and H. Lörz, 1994. Production of doubled-haploid plants from tritordeum anther culture. Theor.Appl.Genet. 87 (6): 741–745.
Barrow, J.R., 1986. The conditions required to isolate and maintain viable cotton (Gossypium hirsutum L.) microspores. Plant Cell Rep. 5: 405–408.
Bensaad, Z.M., M.J. Hennerty and T.D Roche, 1996. Effects of cold pretreatment, carbohydrate source and gelling agents on somatic embryogenesis from anthers of Vitis vinifera L. cvs. ‘Regina’ and ‘Reichensteiner’. Acta Hort. 440: 504–509.
Bhaskaran, S., R.H. Smith and J.J. Finer, 1983. Ribulose bisphosphate carboxylase activity in antherderived plants of Saintpaulia ionantha Wendl. Shag. Plant Physiol. 73: 639–642.
Bicknell, R.A. and N.K. Borst, 1996. Isolation of reduced genotypes of Hieracium pilosella using anther culture. Plant Cell Tiss.Org.Cult. 45 (1): 37–41.
Binding, H. and G. Mordhorst, 1984. Haploid Solanum dulcamara L.: shoot culture and plant regeneration from isolated protoplasts. Plant Sci.Lett. 35: 77–79.
Bingham, E.T., 1969. Haploids from cultivated alfalfa Medicago sativa L. Nature 221: 865–866.
Bingham, E.T. and C.B. Gillies, 1971. Chromosome pairing, fertility, and crossing behavior of haploids of tetraploid alfalfa, Medicago sativa L. Can.J.Genet.Cytol. 13: 195–202.
Birhman, R.K., S.R. Rivard and M. Cappadocia, 1994. Restriction Fragment Length Polymorphism analysis of anther-culture-derived Solanum chacoense. HortSci. 29 (3): 206–208.
Blaschke, J.R., E. Forche and K.H. Neumann, 1978. Investigations on the cell cycle of haploid and diploid tissue cultures of Datura innoxia Mill. and its synchronization. Planta 144: 7–12.
Bohanec, B., 1997. Haploid induction in buckwheat (Fagopyrum esculentum Moench). In: In Vitro Haploid Production in Higher Plants. Vol. 4. Jain,S.M., S.K.Sopory and R.E.Veilleux (Eds.) Kluwer Academic Publishers, Dordrecht, pp. 163–170.
Borgel, A. and M. Arnaud, 1986. Progress in eggplant breeding, use of haplomethod. Capsicum Newsletter 5: 65–66.
Bouvier, L., Y.X. Zhang and Y. Lespinasse, 1993. Two methods of haploidization in pear, Pyrus communis L.: greenhouse seedling selection and in situ parthenogenesis induced by irradiated pollen. Theor.Appl.Genet. 87: 229–232.
Braniste, N., A. Popescu and T. Coman, 1984. Producing and multiplication of Pyrus communis haploid plants. Acta Hortic. 161: 147–150.
Bueno, M.A., A. Gomez, M Boscaiu, J.A. Manzanera and O. Vicente, 1997. Stress-induced formation of haploid plants through anther culture in cork oak (Quercus suber). Physiol.Plant. 99: 335–341.
Bugara, A.M., L.V. Rusina and S. A. Reznikova, 1986. Embryoidogenesis in anther culture of Salvia sclarea. Fiziol.Biokhim.Kult.Rast. 18: 381–386.
Bui-Dang-Ha, D. and J. Pernes, 1982. Androgenesis in pearl millet. I. Analysis of plants obtained from microspore culture. Z.Pflanzenphysiol. 108 (4): 317–327.
Busso, C.S., C.J. Liu, C.T. Hash, J.R. Witcombe, K.M. Devos, J.M. J. de Wet and M.D. Gale, 1995. Analysis of recombination rate in female and male gametogenesis in pearl millet (Pennisetum glaucum) using RFLP markers. Theor.Appl.Genet. 90: 242–246.
Can, N.D., S. Nakamura, T.A.D. Haryanto and T. Yoshida, 1998. Effects of physiological status of parent plants and culture medium composition on the anther culture of sorghum. Plant Prod.Sci.Kyoto 1 (3): 211–215.
Can, N.D. and T. Yoshida, 1999. Combining ability of callus induction and plant regeneration in sorghum anther culture. Plant Prod.Sci.Kyoto 2 (2): 125–128.
Canhoto, J. M. and G. S. Cruz, 1993. Induction of pollen callus in anther cultures of Feijoa sellowiana Berg. (Myrtaceae). Plant Cell Rep. 13 (1): 45–48.
Canhoto, J.M., M. Ludovina, S. Guimaraes and G.S. Cruz, 1990. In vitro induction of haploid, diploid and tetraploid plantlets by anther culture of Iochroma warscewiczii Regel. Plant Cell Tiss.Org.Cult. 21(22): 171–177.
Cao, M.Q., Y. Li, F. Liu and C. Dore, 1994. Embryogenesis and plant regeneration of pakchoi (Brassica rapa L. ssp. chinensis) via in vitro isolated microspore culture. Plant Cell Rep. 13: 447–450.
Cappadocia, M. and M. Ahmim, 1988. Comparison of two culture methods for the production of haploids by anther culture in Solanum chacoense. Can.J.Bot. 66: 1003–1005.
Cappadocia, M., L. Chretien and G. Laublin, 1988. Production of haploids in Gerbera jamesonii.via ovule culture: influence of fall versus spring sampling on callus formation and shoot regeneration. Can.J.Bot. 66: 1107–1110.
Caredda, S. and C. Clement, 1999. Androgenesis and albinism in Poaceae: influence of genotype and carbohydrates. In: Anther and Pollen. From Biology to Biotechnology. Clement, C., E. Pacini and J.-C.Audran (Eds.) Springer-Verlag, Berlin, pp. 211–228.
Cersosimo, A., M Crespan, G. Paludetti and M.M. Altamura, 1990. Embryogenesis, organogenesis and plant regeneartion from anther culture in Vitis. Acta Hort. 280: 307–314.
Chakravarty, B. and S. Sen, 1989. Regeneration through somatic embryogenesis from anther explants of Scilla indica (Roxb.) Baker. Plant Cell Tiss.Org.Cult. 19 (1): 71–75.
Chaudhari, H.K., 1979. The production and performance of doubled haploids of cotton. Bull.Torrey Bot.Club. 106: 123–130.
Chekurov, V.M. and E.P. Razmakhnin, 1999. Effect of inbreeding and growth regulators on the in vitro androgenesis of wheatgrass, Agropyron glaucum. Plant Breed. 118 (6): 571–573.
Chen, Z.G., 1985. A study on induction of plants from citrus pollen. Fruit.Var.J. 39: 44–50.
Chen, Z.H., W.B. Li, L.H. Zhang, X. Xu and S.J. Zhang, 1988. Production of haploid plantlets in cultures of unpolinated ovules of Hevea brasiliensis Muell.-Arg. In: Somatic Cell Genetics of Woody Plants. Ahuja, M. R. (Ed.), Kluwer Academic Publishers, Dordrecht, pp. 39–44.
Choi, B.H., R.Y Park and R.K. Park, 1997. Haploidy in pearl millet [Pennisetum glaucum (L.) R.Br.]. In: In Vitro Haploid Production in Higher Plants. Vol. 4. Jain, S. M., S. K. Sopory and R. E. Veilleux (Eds.) Kluwer Academic Publishers, Dordrecht, pp. 171–179.
Christensen, J. R., E. Borrino, A. Olesen and S. B. Andersen, 1997. Diploid, tetraploid, and octoploid plants from anther culture of tetraploid orchard grass, Dactylis glomerata L. Plant Breed. 116: 267–270.
Collins, G.B., J.M. Dunwell and N. Sunderland, 1974. Irregular microspore formation in Datura innoxia and its relevance to anther culture. Protoplasma 82: 365–378.
Collins, G.B. and N. Sunderland, 1974. Pollen-derived haploids of Nicotiana knightiana, Nicotiana raimondii, and Nicotiana attenuata. J.Exp.Bot. 25: 1030–1039.
Contolini, C.S. and M.Y. Menzel, 1987. Early development of duplication-deficiency ovules in upland cotton. Crop Sci. 27 (2): 345–348.
Corduan, G., 1975. Regeneration of anther-derived plants of Hyoscyamus niger L. Planta 127: 27–36.
Corduan, G., 1976. Isozyme variation as an indicator for the generative or somatic origin of anther-derived plants of Digitalis purpurea L. Z.Pflanzenphysiol. 76 (1): 47–55.
Coumans, M. and D. Zhong, 1995. Doubled haploid sunflower (Helianthus annuus) plant production by androgenesis: fact or artifact? 2. In vitro isolated microspore culture. Plant Cell Tiss.Org.Cult. 41 (3): 203–209.
Couturon, E., 1982. Obtaining naturally occurring haploids of Coffea canephora Piere by grafting of embroys. Cafe,Cacao,Tee. 26: 155–160.
Crane, C.F., W.D. Beversdorf and E.T. Bingham, 1982. Chromosome pairing and associations at meiosis in haploid soybean (Glycine max). Can.J.Genet.Cytol. 24: 293–300.
Cuny, F., B. Dumas de Vaulx, B. Longhi and R. Siadous, 1992. Analyse des plantes de melon (Cucumis melo L) issues de croisements avec du pollen irradie a differentes doses. Agronomie 12: 623–630.
Custers, J.B.M., E. Ennik, W. Eikelboom, J.J.M. Dons and M.M. Van Lookeren Campagne, 1997. Embrogenesis from isolated microspores of tulip; towards developing F1 hybrid varieties. Acta Hort. 430: 259–266.
Dallos, M.P. and V. Galan-Sauco, 1998. Pollen and anther culture in Musa spp. Acta Hort. 490: 493497.
De, D.N. and P.V.L Rao, 1983. Androgenetic haploid callus of tropical leguminous trees. In: Plant Cell Culture in Crop Improvement. Sen, S. K. and K. L. Giles (Eds.) Plenum Press, New York, pp. 469–474.
Debata, B.K. and S.N. Patnaik, 1983. In vitro culture of anther of Crotalaria pallida Ait. for induction of haploid. Indian J.Exp.Biol. 21: 44–46.
Debata, B.K. and S.N. Patnaik, 1988. Induction of androgenesis in anther cultures of Solanum viarum Dunal. J.Plant Physiol. 133 (1): 124–125.
DeLange, J.H. and A.P. Vincent, 1988. Studies on Citrus pollination using gamma-irradiated pollen. S.Afr.J.Bot. 54 (3): 257–264.
Denissen, C.J.M. and A.P.M. Den Nijs, 1987. Effects of gamma irradiation on in vitro pollen germination of different Cucumis species. Euphytica 36: 651–658.
DeVerna, J.W. and G.B. Collins, 1984. Maternal haploids of Petunia axillaris (Lam.) B.S.P. via culture of placenta attached ovules. Theor.Appl.Genet. 69: 187–192.
Diettrich, B., S. Ernst, and M. Luckner, 2000. Haploid plants regenerated from androgenic cell cultures of Digitalis Janata. Planta Med. 66 (3): 237–240.
Dieu, P. and J.M. Dunwell, 1988. Anther culture with different genotypes of opium poppy (Papaver somniferum L.): effect of cold treatment. Plant Cell Tiss.Org.Cult. 12 (3): 263–271.
Dryanovska, O.A. and I.N. Ilieva, 1983. In vitro anther and ovule cultures in muskmelon (Cucumis melo L.). Comptes Rendus de l’Academie Bulgare de Sciences 36(8): 1107–1110.
Du, L. G., Q.Q. Shao and A.S. Li, 1986. Somatic embryogenesis and plant regeneration from anther culture of Panax quinquifolius (ginseng). Newslet.Int.Plant Biotech.Network. 6: 9.
Dublin, P., 1978. Diploidized haploids and production of fertile homozygous genotypes in cultivated cacao trees (Theobroma cacao). Cafe,Cacao,Tee 22: 275–284.
Dumas-de-Vaulx, R., 1979. Obtaining haploid plants in the melon (Cucumis melo L.) after pollination by Cucumis ficifolius A. Rich. C.R.Hebd.Seances Acad.Sci.Ser.D.Sci.Nat. 289: 875–878.
Dumas-de-Vaulx, R. and D. Chambonnet, 1986. Obtention of embryos and plants from in vitro culture of unfertilized ovules of Cucurbita pepo. In: Genetic Manipulation in Plant Breeding. Horn, W., C.J. Jensen, W. Oldenbach and O. Schieder (Eds.) Walter de Gruyter and Co, Berlin, pp. 295–297.
Dumas-de-Vaulx, R., D. Chambonnet and E. Pochard, 1981. In vitro culture of pepper (Capsicum annuum L.) anthers: high rate plant production from different genotypes by +35 degrees Celsius treatments haploid and diploid plants, cultivars. Agron.Sci.Prod.Veg.Environ. 1: 859–864.
Dunwell, J.M. and N. Sunderland, 1976. Pollen ultrastructure in anther cultures of Datura innoxia. III. Incomplete microspore division. J.Cell Sci. 22: 493–501.
Elkonin, L.A., T.N. Gudova, A.G. Ishin, and U.S. Tyrnov, 1993. Diploidization in haploid tissue cultures of sorghum. Plant Breed. 110: 201–206.
Emershad, R.L., D.W. Ramming and M.D Serpe, 1989. In ovulo embryo development and plant formation from stenospermic genotypes of Vitis vinifera. Aust.J.Bot. 76(3): 397–402. Evans,D.A. and R.Morrison. (1989) Tomato anther culture. (US 4 835 339).
Falque, M., 1994. Pod and seed development and phenotype of M1 plants after pollination and fertilization with irradiated pollen in cacao (Theobroma cacao L.). Euphytica 75: 19–25.
Falque, M., A.A. Kodia, O. Sounigo, A.B. Eskes, and A. Charrier, 1992. Gamma-irradiation of cacao (Theobroma cacao L.) pollen: Effect of pollen viability, germination and mitosis on fruit set. Euphytica 64 (3): 167–172.
Fan, Y.H., S.Y. Zang and J.F. Zhao, 1982. Induction of haploid plants in Lycium chinense Mill. and Lycium barbarum by anther culture. I. Chuan. Hereditas 4: 25–26.
Fankhauser, H., F. Bucher and P.J. King, 1984. Isolation of biochemical mutants using haploid mesophyll protoplasts of Hyoscyamus muticus. IV. Biochemical characterisation of nitrate non-utilizing clones. Planta 160: 415–421.
Faure, O., J. Aarrouf and A Nougarede, 1996a. Ontogenesis, differentiation and precocious germination in anther-derived somatic embryos of grapevine (Vitis vinifera L.): embryonic organogenesis. Ann.Bot. 78 (1): 29–37.
Faure, O., J. Aarrouf and A Nougarede, 1996b. Ontogenesis, differentiation and precocious germination in anther-derived somatic embryos of grapevine (Vitis vinifera L.): proembryogenesis. Ann.Bot. 78 (1): 23–28.
Fedak, G., 1983. Haploids in Triticum ventricosum via intergeneric hybridization with Hordeum bulbosum. Can.J.Genet.Cytol. 25: 104–106.
Ficcadenti, N., S. Sestili, S. Annibali, M. di Marco and M. Schiavi, 1999. In vitro gynogenesis to induce haploid plants in melon Cucumis melo L. J.Genet.and Breed. 53(3): 255–257.
Fitch, M.M. and P.H. Moore, 1984. Production of haploid Saccharum spontaneum L. Comparison of media for cold incubation of panicle branches and for float culture of anthers. J.Plant Physiol. 117: 169–178.
Fitch, M.M.M. and P.H. Moore, 1996. Haploids of sugarcane. In: In Vitro Haploid Production in Higher Plants. Jain, S.M., S.K. Sopory and R.E. Veilleux (Eds.) Kluwer Academic Publishers, Dordrecht, pp. 1–16.
Forche, E., R. Kibler and K.H. Neumann, 1981. The influence of developmental stages of haploid and diploid callus cultures of Datura innoxia on shoot initiation. Z.Pflanzenphysiol. 101: 257–262.
Fougat, R.S., A.R. Pathak and P.S. Bharodia, 1992. Regeneration of haploid callus from anthers of pigeonpea. Gujarat Agr.Univ.Res.J. 17 (2): 151–152.
Fraser, L.G. and C.F. Harvey, 1986. Somatic embryogenesis from anther-derived callus in two Actinidia species. Sci.Hortic. 29: 335–346.
Frederiksen, S., 1989. Chromosome elimination in a hybrid between Taeniatherum caput-medusae and Hordeum bulbosum. Hereditas 110: 87–88.
Friedt, W., T. Nurhidayah, T. Rocher, H. Kohler, R Bergmann and R. Horn, 1997. Haploid production and application of molecular methods in sunflower (Helianthus annuus L.). In: In Vitro Haploid Production in Higher Plants. Vol. 5. Jain, S.M., S.K. Sopory and R.E. Veilleux (Eds.) Kluwer Academic Publishers, Dordrecht, pp. 17–35.
Froelicher, Y. and P. Ollitrault, 2000. Effects of the hormonal balance on Clausena excavata androgenesis. Acta Hort. 535: 139–146.
Fu, L.F. and D.Y. Tong, 1983. Induction pollen plants of litchi tree (Litchi chinensis Soon). Acta Genet. Sin. 10 (5): 369–374.
Gaj, M. and M.D. Gaj, 1985. Dihaploids of Hordeum murinum L. and H. secalinum Schreb. from interspecific crosses with H. bulbosum L. Barley Genet.Newsl. 15: 33–34.
Gavrilenko, T., R. Thieme and V.-M. Rokka, 2001. Cytogenetic analysis of Lycopersicon escullentum (+) Solanum etuberosum somatic hybrids and their androgenetic regenerants. Theor.Appl.Genet. 103: 231–239.
Gelebart, P. and L.H. San, 1987. Production of haploid plants of sunflower (Helianthus annuus L.) by in vitro culture of nonfertilized ovaries and ovules. Agron.Sci.Prod.Veg.Environ. 7: 81–86.
Geraci, G. and A. Starrantino, 1990. Attempts to regenerate haploid plants from “ in vitro” cultures of citrus anthers. Acta Hort. 280: 315–320.
Gerassimowa, H., 1936. Experimantell erhaltene haploide Pflanze von Crepis tectorum L. Planta. 25: 696–702.
Germana, M.A., 1997. Haploidy in Citrus. In: In Vitro Haploid Production in Heigher Plants. Vol. 5. Jain, S.M., S.K. Sopory and R.E. Veilleux (Eds.) Kluwer Academic Publishers, Dordrecht, pp. 195–217.
Germana, M.A., F.G Crescimanno, F. de Pasquale and W.Y. Ying, 1992. Androgenesis in 5 cultivars of Citrus limon L. Burm. f. Acta Hort. 300: 315–324.
Germana, M.A., F.G Crescimanno and A. Motisi, 2000a. Factors affecting androgenesis in Citrus clementina Hort. ex. Tan. Adv.Hort.Sci. 14 (2): 43–51.
Germana, M.A., F.G Crescimanno, G.R. Recupero and M.P. Russo, 2000b. Preliminary characterization of several doubled haploids of Citrus clementina cv. Nules. Acta Hort. 535: 183–190.
Germana, M.A., Y.Y. Wang, M.G. Barbagallo, G. Iannolino and F.G. Crescimanno, 1994. Recovery of haploid and diploid plantlets from anther culture of Citrus clementina Hort. ex Tan. and Citrus reticulata Blanco. J.Hortic.Sci. 69 (3): 473–480.
Gharyal, P.K., A. Rashid and S.C. Maheshwari, 1983a. Androgenic response from cultured anthers of a leguminous tree, Cassia siamea Lam. Protoplasma 118: 91–93.
Gharyal, P.K., A. Rashid and S.C. Maheshwari, 1983b. Production of haploid plantlets in anther cultures of Albizzia lebbeck L. Plant Cell Rep. 2: 308–309.
Gmitter, F.G. Jr. and G.A. Moore, 1986. Plant regeneration from undeveloped ovules and embryogenic calli of Citrus: embryo production, germination, and plant survival. Plant Cell Tiss.Org.Cult. 6 (2): 139–147.
Gonzalez-Melendi, P., P.S. Testillano, P. Ahmadian, B. Fadon, O. Vicente and M.C. Risueno, 1995. In situ characterization of the late vacuolate microspore as a convenient stage to induce embryogenesis in Capsicum. Protoplasma 187(14): 60–71.
Gu, Z.P. and K.C. Cheng, 1983. In vitro induction of haploid plantlets from unpollinated young ovaries of lily and its embryological observations (Lilium davidii). Acta Bot.Sinica 25: 24–28.
Gudu, S., J.D. Procunier, A. Ziauddin and K. J. Kasha, 1993. Anther culture derived homozygous lines in Hordeum bulbosum. Plant Breed. 110: 109–115.
Guedira, M., T. Dubois-Tylski, J. Vasseur and J. Dubois, 1989. Direct somatic embryogenesis obtained from anther culture of Cichorium (Asteraceae). Can.J.Bot. 67: 970–976.
Gulshan, T.M. Varghese and D.R. Sharma, 1981. Studies on anther cultures of tomato–Lycopersicon esculentum Mill. Biol.Plant. 23 (6): 414–420.
Gupta, P.P., 1983. Microspore-derived haploid, diploid and triploid plants in Petunia violacea Lindl. Plant Cell Rep. 2: 255–256.
Gupta, S., 1975. Morphogenetic response of haploid callus tissue of Pisum sativum (var. B22). Indian Agriculturist 19 (4): 11–21.
Gurel, A., K. Nichterlein and W. Friedt, 1991. Shoot regeneration from anther culture of sunflower (Helianthus annuus) and some interspecific hybrids as affected by genotype and culture procedure. Plant Breed. 106 (1): 68–76.
Hammerschlag, F.A., 1993. Factors affecting the frequency of callus formation among cultured peach anthers. HortSci. 18 (2): 210–211.
Han, D.S., Y. Niimi and M Nakano, 1997. Regeneration of haploid plants from anther cultures of the Asiatic hybrid lily ‘Conecticut King’. Plant Cell Tiss.Org.Cult. 47: 153–158.
Han, D.S., Y. Niimi and M. Nakano, 1999. Production of doubled haploid plants through colchicine treatment of anther-derived haploid calli in Asiatic hybrid lily ‘Connecticut King’. J.Japanese Soc.Hortic.Sci. 68 (5): 979–983.
Hanson, M.R., 1984. Anther and pollen culture. Monogr.Theor.Appl.Genet. 9: 138–150.
Hatano, K., Y. Shoyama and I. Nishioka, 1987. Somatic embryogenesis and plant regeneration from the anther of Aconitum carmichaeli Debx. Plant Cell Rep. 6 (6): 446–448.
Haydu, Z. and I.K. Vasil, 1981. Somatic embryogenesis and plant regeneration from leaf tissues and anthers of Penisetum purpureum Schum. Theor.Appl.Genet. 59 (5): 269–273.
Hennerty, M.J. and A.J. Sayegh, 1996. Polyhaploidy in strawberry. In: In Vitro Haploid Production in Higher Plants. Jain, S.M., S.K. Sopory and R.E. Veilleux (Eds.) Kluwer Academic Publishers, Dordrecht, pp. 231–260.
Hermsen, J.G.T., 1969. Induction of haploids and aneuhaploids in colchicine-induced tetraploid Solanum chacoense Bitt. Euphytica 18: 183–189.
Hesemann, C.U., 1980. Haploid cells in calluses from anther culture of Vicia faba. Z.Pflanzenzüchtg. 84: 18–27.
Hidaka, T., 1984. Induction of plantlets from anthers of ‘Trovita’ orange (Citrus sinensis Osbeck). J.Japanese Soc.Hortic.Sci. 53 (1): 1–5.
Hidaka, T., Y.Yamada and T. Shichijo, 1979. In vitro differentiation of haploid plants by anther culture in Poncirus trifoliata (L.) Raf. Japan.J.Breed. 29: 248–254.
Hidaka, T., Y.Yamada and T. Shichijo, 1982. Plantlet formation by anther culture of Citrus aurantium L. Induction of haploid plants. Japan.J.Breed. 32: 247–252.
Hildebrand, D.F., G.C. Phillips and G.B. Collins, 1986. Soybean [Glycine max (L.) Men.]. In: Biotechnology in Agriculture and Forestry 2. Crops I. Bajaj, Y.P.S. (Ed.) Springer-Verlag, Berlin,pp. 283–308.
Hinata, K., 1986. Egg Plant (Solanum melongena L.). In: Biotechnology in Agriculture and Forestry 2. Crop I. Bajaj, Y.P.S. (Ed.) Springer-Verlag, Berlin, pp. 363–370.
Hinchee, M.A.W., A. dela Cruz and A. Maretzki, 1984. Development and biochemical characteristics of cold-treated anthers of Saccharum spontaneum. J.Plant Physiol. 115 (4): 271–284.
Hollo, R. and S. Misik, 2000. Investigations of grape anther culture aiming haploid plant production. Acta Hort. 528: 347–350.
Hongyuan, Y., Z. Chang, C. Detian, Y. Hua, W. Yan and C. Xiaoming, 1986. In vitro culture of unfertilized ovules in Helianthus annuus L. In: Haploids of Higher Plants In Vitro. Hu, H. and H. Yang ( Eds.) China Academic Publishers, Beijing, pp. 182–191.
Honkanen, J., A. Aapola, P. Seppanen, T. Tormala, J.0 de Wit, L.J.M. Strayers and H.F. Esendam, 1992. Production of doubled haploid gerbera clones. Acta Hort. 300: 341–346.
Höfer, M. and V. Hanke, 1990. Induction of androgenesis in vitro in apple and sweet cherry. Acta Hort. 280: 333–336.
Hu, C.Y., G.C. Yin, M. Helena and B Zanettini, 1996. Haploid of soybean. In: In vitro Haploid Production in Higher Plants. Vol. 3. Jain, S.M., S.K. Sopory and R.E. Veilleux (Eds.) Kluwer Academic Publishers, Dordrecht, pp. 377–395.
Huda, S., R. Islam, M.A. Bari and M. Asaduzzaman, 2001. Anther culture of chickpea. Int.Chickpea and Pigeonpea Newslet. 8: 24–26.
Hughes, K. W., S. L. Bell and J. D. Caponetti, 1975. Anther-derived haploids of the African violet. Can.J.Bot. 53: 1442–1444.
Humphreys, M.W., A.G. Zare, I. Pasakinskiene, H. Thomas, W.J. Rogers and H.A. Collin, 1998. Interspecific genomic rearrangements in androgenic plants derived from a Lolium multiflorum x Festuca arundinacea (2n=5x=35) hybrid. Heredity, 80: 78–82.
Hwang, J.K., K.Y. Paek and D.H. Cho, 1998. Breeding resistant pepper lines (Capsicum annuum L.) to bacterial spot (Xanthomonas campestris pv. vesicatoria) through anther culture. Acta Hort. 461: 301–307.
Hyun, S.K., J.H. Kim, E.W. Noh and J.I. Park, 1986. Induction of haploid plants of Populus species. In: Plant Tissue Culture and Its Agricultural Applications. Withers, L.A. and P.G. Alderson (Eds.) Butterworths, London, pp. 413–418.
lankulov, I.K., M.D. Abadzhieva and A.I. Atanasov, 1979. Investigation of experimentally obtained haploid from Datura innoxia Mill. Dokl.Bulg.Akad.Nauk 32: 213–216.
Ikeda, M., T. Masuda, T. Kotobuki, T. Yoshioka, T. Nakanishi and M. Yoshida, 1993. Artificial control of polyembryogenesis and production of hybrids by chronic gamma irradiation on Citrus ovule in vitro. Bull.Natl.Inst.Agrobiol.Resour. 8: 25–46.
Imelda, M., S.H.A. Lubis and S. Sastrapradja, 1988. Anther culture of rambutan (Nephelium sp.). Annales Bogorienses, New Series 1 (1): 7–9.
Inagaki, N., H. Matsunaga, M. Kanechi and S. Maekawa, 1994. In vitro micropropagation of Allium giganteum R. 2. Embryoid and plantlet regeneration through the anther culture of Allium giganteum R. Sci.Rep.Fac.Agric.Kobe.Univ. 21(1): 23–30.
Ishizaka, H., 1998. Production of microspore-derived plants by anther culture of an interspecific F1 hybrid between Cyclamen persicum and C. purpurascens. Plant Cell Tiss.Org.Cult. 54: 21–28.
Isouard, G., C. Raquin and Y. Demarly, 1979. Haploid and diploid plants obtained by culture in vitro from eggplant anthers (Solaum melongena). C.R.Hebd.Seances Acad.Sci.Ser.D.Sci.Nat. 288: 987–989.
Jain, A.K., A. Sarkar and R. K Datta, 1996. Induction of haploid callus and embryogenesis in in vitro cultured anthers of mulberry (Morus indica). Plant Cell Tiss.Org.Cult. 44 (2): 143–147.
Jain, R.K., U. Brune and W. Friedt, 1989. Plant regeneration from in vitro cultures of cotyledon explants and anthers of Sinapis alba and its implications on breeding of crucifers. Euphytica 43 (12): 153–163.
Jain, S.M. and N. Bhalla-Sarin, 1997. Haploidy in Petunia. In: In Vitro Haploid Production Production in Higher Plants. Vol. 5. Jain, S.M., S.K. Sopory and R.E. Veilleux (Eds.) Kluwer Academic Publishers, Dordrecht, pp. 53–71.
Jaiswal, V.S. and P. Narayan, 1981. Induction of pollen embryoids in Solanum torvum Swartz. Haploid induction. Curr.Sci. 50: 998–999.
Jayasree, P.K., M.P. Asokan, S. Sobha, L. S. Ammal, K. Rekha, R. G. Kala and R. Jayasrree, 1999. Somatic embryogenesis and plant regeneration from immature anthers of Hevea brasiliensis (Muell.) Arg. Curr.Sci. 76 (9): 1242–1245.
Jelenkovic, G., O. Shifriss and E. Harrington, 1980. Association and distribution of meiotic chromosomes in a haploid of Ricinus communis L. Cytologia 45: 571–577.
Jelenkovic, G., M.L. Wilson and P.J. Harding, 1984. An evaluation of intergeneric hybridization of Fragaria spp. x Potentilla spp. as a means of haploid production. Euphytica, 33: 143–152.
Jian, Y.Y., D.P. Liu, X.M. Luo and G.L. Zhao, 1986. Studies on induction of pollen plants in Glycine max (L.) Merr. Chiang.su.Nung.Yeh.Hseuh.Pao.J.Agric.Sci. 2: 26–30.
Johansson, L.B., 1986. Effects of activated charcoal, cold treatment and elevated CO2-concentrations on embryogenesis in anther cultures. In: Genetic Manipulation in Plant Breeding. Horn, W., C.J. Jensen, W. Oldenbach and O. Schieder (Eds.), Walter de Gruyter and Co, Berlin, pp. 257–264.
Johansson, L.B., E. Calleberg, and A. Gedin, 1990. Correlation between activated charcoal, Fe-EDTA and other organic media ingredients in cultured anthers of Anemone canadensis. Physiol.Plant. 80 (2): 243–249.
Jorgensen, J., 1991. Androgenesis in Quercus petraea, Fagus sylvatica, and Aesculus hippocastanum. NATO.Adv.Sci.Inst.Ser.Ser.A.Life Sci. 210: 323–354.
Jorgensen, R. B. and R. Bothmer, 1988. Haploids of Hordeum vulgare and H. marinum from crosses between the two species. Hereditas 108: 207–212.
Kadlec, M., J. Suchomelova, V. A Smirnov, and S. L. Nikolajevna, 1991. Anther culture in soybean. Soybean Genet.Newsl. 18: 121–124.
Kaltchuk-Santos, E., J.E. Mariath, E. Mundstock, C.-Y. Hu, and M.H. Bodanese-Zanettini, 1997. Cytological analysis of early microspore divisions and embryo formation in cultured soybean anthers. Plant Cell Tiss.Org.Cult. 49: 107–115.
Kasperbauer, M.J. and R.C. Buckner, 1979. Haploid plants from anthers of Festuca arundinacea cultured with nurse tissue. Agron.Abstr. pp. 66.
Kato, M., T. Suga, and S. Tokumasu, 1980. Effect of 2,4-D and NAA on callus formation and haploid production in anther culture of Pelargonium roseum. Mem.Coll.Agric.Ehime.Univ. 24: 199–207.
Kaur, P. and J.K. Bhalla, 1998. Regeneration of haploid plants from microspore culture of pigeon pea (Cajanus cajan L.). Indian J.Exp.Biol. 36 (7): 736–738.
Kavi Kishor, P.B., T.P. Reddy, A. Sarvesh, and G. Venkatesham, 1997. Haploidy in niger (Guizotia abyssinica Cass). In: In Vitro Haploid Production in Higher Plants. Jain, S.M., S.K. Sopory and R.E. Veilleux (Eds.) Kluwer Academic Publishers, Dordrecht, pp. 37–51.
Kenny, L. and P.D.S. Caligari, 1996. Androgenesis of the salt tolerant shrub Atriplex glauca. Plant Cell Rep. 15: 829–832.
Khan, S.K. and P.D. Ghosh, 1983. In vitro induction of androgenesis and organogenesis in Cicer arietinum L. Current Sci. 52 (18): 891–893.
Khoder, M., P. Villemur, and R. Jonard, 1984. Obtainment of monoploid and triploid plants by in vitro androgenesis in Begonia x hiemalis Fotsch cv. (A). Bull.Soc.Bot.Fr.Lett.Bot. 131: 43–48.
Khushwinder, S., B.S. Sandhu, and S.S. Gosal, 1998. Anther culture response in cotton. Annals Biol.Ludhiana 14 (1): 11–13.
Kihara, M., K. Fukuda, H. Funatsuki, I. Kishinami, and Y. Aida, 1994. Plant regeneration through anther culture of tree wilde species of Hordeum (H. murinum, H. marinum, and H. bulbosum). Plant Breed. 112: 244–247.
Kim, C.K., J.Y. Oh, and J.D. Chyng, 1998. Plant regeneration of Korean native Chinese chive by unpollinated ovule culture. J.Korean Soc.Hortic.Sci. 39 (6): 693–696.
Kim, J.H., H.K. Moon, and J.I. Park, 1986. Haploid plantlet induction through anther culture of Populus maximowiczii. Yon’gu.Pogo.Res.Rep.Inst.For.Genet. 116–121.
Kim, J.H., E.W. Noh, and J.I. Park, 1983. Haploid plantlets formation through anther culture of Populus glandulosa. Yon’gu.Pogo.Res.Rep.Inst.For.Genet. 93–98.
Kim, S.W., N.H. Song, K.H Jung, S.S. Kwak, and J.R. Liu, 1994. High frequency plant regeneration from anther-derived cell suspension cultures via somatic embryogenesis in Catharanthus roseus. Plant Cell Rep. 13 (6): 319–322.
Kintzios, S. and G. Fischbeck, 1994. Anther culture response of Hordeum spontaneum-derived winter barley lines. Plant Cell Tiss.Org.Cult. 37: 165–170.
Kiss, J., M. Kondrak, O. Torjek, E. Kiss, G. Gyulai, K. Mazik Tokei, and L.E. Heszky, 2001. Morphological and RAPD analysis of poplar trees of anther culture origin. Euphytica 118: 213–221
Kiviharju, E. and E. Pehu, 1998. The effect of cold and heat pretreatments on anther culture response of Avena sativa and A. sterilis. Plant Cell Tiss.Org.Cult. 54: 97–104.
Kiviharju, E., M. Puolimatka, and E. Pehu, 1997. Regeneration of anther-derived plants of Avena sterilis. Plant Cell Tiss.Org.Cult. 48: 147–152.
Koltunow, A.M., K. Soltys, N. Nito, and S. McClure, 1995. Anther, ovule, seed, and nuclear embryo development in Citrus sinensis cv. Valencia. Can.J.Bot. 73 (10): 1567–1582.
Kristiansen, K. and S.B. Andersen, 1993. Effects of donor plant temperature, photoperiod, and age on anther culture response of Capsicum annuum L. Euphytica 67 (1/2): 105–109.
Krumbiegel, G., 1979. Response of haploid and diploid protoplasts from Datura innoxia Mill. and Petunia hybrida L. to treatment with X-rays and a chemical mutagen. Environ.Exp.Bot. 19: 99–103.
Kumaravadivel, N. and S.R. Sree Rangasamy, 1994. Plant regeneration from sorghum anther cultures and field evaluation of progeny. Plant Cell Rep. 13: 286–290.
Kusum, M. and S.C. Maheshwari, 1977. Enhancement by cold treatment of pollen embryoid development in Petunia hybrida. Z.Pflanzenphysiol. 85 (2): 177–180.
Kwack, S.N. and K. Fujieda, 1988. Somatic embryogenesis in cultured unfertilized ovules of Cucurbita moschata. J.Japanese Soc.Hortic.Sci. 57 (1): 34–42.
Lanaud, C., P. Lachenaud, and O. Sounigo, 1988. Behavior in the growth of doubled hapolids of cacao plants (Theobroma cacao). Can.J.Bot. 66: 1986–1992.
Lashermes, P., E. Couturon, and A Charrier, 1994a. Combining ability of doubled haploids in Coffea canephora P. Plant Breed. 112: 330–337.
Lashermes, P., E. Couturon, and A. Charrier, 1994b. Doubled haploids of Coffea canephora: development, fertility and agronomic characterictics. Euphytica 74: 149–157.
Laurain, D., J. Tremouillaux-Guiller, and J.-C. Chenieux, 1993. Embryogenesis from microspores of Ginkgo biloba L., a medicinal woody species. Plant Cell Rep. 12: 501–505.
Lazarte, J.E. and C.C. Sasser, 1982. Asexual embryogenesis and plant development in anther culture of Cucumis sativus L. HortSci. 17 (1): 88.
Le Deunff, E. and A. Sauton, 1994. Effect of parthenocarpy on ovule development in cucumber (Cucumis sativus L.) after pollination with normal and irradiated pollen. Sex.Plant Reprod. 7: 221–228.
Le Thi, K., R. Lespinasse, S. Siljak-Yakovlev, T. Robert, N. Khalfallah, and A. San, 1994. Karyotypic modifications in androgenetic plantlets of pearl millet, Pennisetum glaucum (L.) R. Brunken: occurrence of B chromosomes. Caryologia 47: 1–10.
Lee, B.K., J.A. Ko, and Y.S. Kim, 1992. Studies on the thidiazuron of anther culture of Paeonia albiflora. J.Korean Soc.Hortic.Sci. 33 (5): 384–395.
Lee, S.S. and Y.J. Yoon, 1987. Anther culture of XBrassicoraphanus. Cruciferae Newsl. 12: 68.
Lefebvre, V., A. Palloix, C Caranta, and E. Pochard, 1994. Construction of an interspecific integrated linkage map of pepper using molecular markers and doubled-haploid progenies. Genome 38: 112–121.
Lesniewska, A., A. Ponitka, A. Slusarkiewicz-Jarzina, E. Zwierzykowska, Z. Zwierzykowski, A.R. James, H. Thomas, and M.W. Humphreys, 2001. Androgenesis from Festuca pratensis x Lolium multiflorum amphidiploid cultivars in order to select and stabilize rare gene combinations for grass breeding. Heredity 86: 167–176.
Li, C.L. and B.J. Ye, 1995. Successful development of new sweet (hot) pepper cultivars by anther culture. Acta Hort. 402: 442–444.
Li, S.Z., W.P. Wu, Z.H. Zhang, and D.Y. Wang, 1988. Study on anther culture of strawberry (Fragaria ananassa). Genet.Manipulation Crops.Newsl. 4 (1): 52–62.
Liang, G.H., X. Gu, G.L. Yue, Z.S. Shi, and K.D. Kofoid, 1997. Haploidy in sorghum. In: In Vitro Haploid Production in Higher Plants. Jain, S.M., S.K. Sopory and R.E. Veilleux (Eds.) Kluwer Academic Publishers, Dordrecht, pp. 149–161.
Lichter, R., 1989. Efficient yield of embryoids by culture of isolated microspores of different Brassicaceae species. Plant Breed. 103: 119–123.
Ling, J.T., M. Iwamasa, and N. Nito, 1988. Plantlet regeneration by anther culture of Calamondin (Citrus madurensis Lour.). In: Citriculture. Proc. Sixth Int. Citrus Congress, Middle East. Vol. 1. Balaban Publishers, Rehovot, pp. 251–256.
Liu, M.C. and W.H. Chen, 1978. Organogenesis and chromosome number in callus derived from cassava anthers. Can.J.Bot. 56 (10): 1287–1290.
Liu, M.C., W.H. Chen, and L.S. Yang, 1980. Anther culture in sugarcane. I. Structure of anther and its pollen grain development stages. Taiwan Sugar. 27 (3): 86–91.
Livingston, G.K., 1971. Experimental studies on the induction of haploid parthenogenesis in Douglas-fir and the effects of radiation on the germination and growth of Douglas-fir pollen. Ph.D. Thesis, Univ.of Washington, Seattle, pp. 189.
Long, C.M., C.A. Mullinix, and A.F. Iezzoni, 1994. Production of a microspore-derived callus population from sweet cherry. HortSci. 29 (11): 1346–1348.
Lysenko, E.G. and V.A. Sidorov, 1985. Obtainment of androgenous Solanum bulbocastanum haploids and mesophyll protoplast culture. Cytol.Genet. 19: 35–37.
Maheswary, V. and C. Mak, 1993. The influence of genotypes and environments on induction of pollen plants for anther culture of Capsicum annuum L. AsPac.J.Mol.Biol.Biotechnol. 1 (1): 4350.
Mahill, J.F., J.N. Jenkins, J.C.J. McCarty, and W.L. Parrott, 1984. Performance and stability of doubled haploid lines of upland cotton derived via semigany. Crop Sci. 24: 271–277.
Makhmudov, T., 1978. Use of haploids in cotton breeding. Khlopkovodstvo pp. 31–32
Marburger, J.E. and R.R.C. Wang, 1988. Anther culture of some perennial triticeae. Plant Cell Rep. 7: 313–317.
Marinkovic, N. and L. Radojevic, 1992. The influence of bud length, age of the tree and culture media on androgenesis induction in Aesculus carnea Hayne anther culture. Plant Cell Tiss.Org.Cult. 31 (1): 51–59.
Martinez, L.D. and N.I. de Halac, 1995. Organogenesis of anther-derived calluses in long-term cultures of Oenotera hookeri de Vries. Plant Cell Tiss.Org.Cult. 42: 91–96.
Matsubara, S., N. Dohya, and K. Murakami, 1995. Callus formation and regeneration of adventitious embryos from carrot, fennel and mitsuba microspores by anther and isolated microspore cultures. Acta Hort. 392: 129–137.
Mauro, M.C., C. Nef, and J. Fallot, 1986. Stimulation of somatic embryogenesis and plant regeneration from anther culture of Vitis vinifera cv. Cabernet-Sauvignon. Plant Cell Rep. 5: 377–380.
Mazzolani, G., G. Pasqua, and B. Monacelli, 1979. Conditions for the development of haploid plants from in vitro cultures of pollen grains Atropa belladonna, Nicotiana tabacum, tobacco. Ann.Bot.Rome. 38: 107–117.
Mehetre, S.S., 1984. Analysis of chromosome pairing in haploids of cotton (Gossypium spp.). Indian J.Agric.Res. 18: 49–53.
Mehetre, S.S. and M.V. Thombre, 1981. Meiotic studies in the haploids (2n=2X=26) of the tetraploid cottons (2n=4X=52) Gossypium hirsutum, Gossypium barbadense. Proc.Indian Natl. Sci.Acad. Part.B.Biol. Sci. 47: 516–518.
Mehetre, S.S. and M.V. Thombre, 1982. Cytomorphology of haploid Gossypium hirsutum X Gossypium anomalum Cotton. Indian J.Genet.Plant Breed. 42: 144–149.
Meredith, W.R., R.R. Bridge, and J. F. Chism, 1970. Relative performance of F1 and F2 hybrids from doubled haploids and their parent varieties in upland cotton, Gossypium hirsutum L. Crop Sci. 10: 295–298.
Metwally, E.I., S.A. Moustafa, B.I. El-Sawy, and T.A. Shalaby, 1998a. Haploid plantlets derived by anther culture of Cucurbita pepo. Plant Cell Tiss.Org.Cult. 52: 171–176.
Metwally, E.I., S.A. Mustafa, B.I. El-Sawy, S.A. Haroun, and T.A. Shalaby, 1998b. Production of haploid plants from in vitro culture of inpollinated ovules of Cucurbita pepo. Plant Cell Tiss.Org.Cult. 52 (3): 117–121.
Meynet, J., R. Barrade, A. Duclos, and R. Siadous, 1994. Dihaploid plants of roses (Rosa x hybrida, cv. ‘Sonia’) obtained by parthenogenesis induced using irradiated pollen and in vitro culture of immature seeds. Agronomie 2: 169–175.
Meynet, J. and M. Sibi, 1984. Haploid plants from in vitro culture of unfertilized ovules in Gerbera jamesonii. Z.Pflanzenzüchtg. 93: 78–85.
Mezzarobba, A. and R. Jonard, 1986. Effects of the stage of isolation and pretreatments on in vitro development of cultivated sunflower anthers (Helianthus annuus L.). C.R.Acad.Sci.Ser.III.Sci.Vie. 303 (5): 181–186.
Miki-Hirosige, H., S. Nakamura, and I. Tanaka, 1988. Ultrastructural research on cell wall regeneration by cultured pollen protoplasts of Lilium longiflorum. Sex.Plant Reprod. 1: 36–45.
Misra, N.R., T.M. Varghese, N. Maherchandani, and R.K. Jain, 1983. Studies on induction and differentiation of androgenic callus of Solanum melongena L. In: Plant Cell Culture in Crop Improvement. Sen, S.K. and K.L. Giles (Eds.) Plenum Press, New York pp. 465–468.
Mityko, J., A. Andrasfalvy, G. Csillery, and M. Fari, 1995. Anther-culture response in different genotypes and F1 hybrids of pepper (Capsicum annuum L.). Plant Breed. 114 (1): 78–80.
Miyoshi, K., 1996. Callus induction and plantlet formation through culture of isolated microspores of eggplant (Solanum melongena L.). Plant Cell Rep. 15 (6): 391–395.
Miyoshi, K. and N. Asakura, 1996. Callus induction, regeneration of haploid plants and chromosome doubling in ovule cultures of pot gerbera (Gerbera jamesonii). Plant Cell Rep. 16: 1–5.
Mol, R., 1992. In vitro gynogenesis in Melandrium album: from parthenogenetic embryos to mixoploid plants. Plant Sci. 81(2):261–269.
Monfort, S., 1985. Androgenesis of coconut: embryos from anther culture. Z.Pflanzenzüchtg. 94: 251–254.
Moriguchi, T., M. Omura, N. Matsuta, and I.Kozaki, 1987. In vitro adventitious shoot formation from anthers of pomegranate. HortSci. 22: 947–948.
Morrison, R.A., R.E. Koning, and D.A. Evans, 1986. Anther culture of an interspecific hybrid of Capsicum. J.Plant Physiol. 126: 1–9.
Mosquera, T., L.E. Rodriguez, A. Parra, and M. Rodriguez, 1999. In vitro adventive regeneration from carnation (Dianthus caryophyllus) anthers. Acta Hort. 482: 305–308.
Motzo, R. and M. Deidda, 1993. Anther and ovule culture in globe artichoke. J.Genet.and Breed. 47 (3): 263–266.
Mozsar, J. and S. Sule, 1994. A rapid method for somatic embryogenesis and plant regeneration from cultured anthers of Vitis riparia. Vitis. 33 (4): 245–246.
Mukherjee, A., M. Unnikrishnan, and N.G. Nair, 1991. Callus induction, embryogenesis and regeneration from sweet potato anther. J.Root Crops. 17: 302–304.
Muniswamy, B. and H.L. Sreenath, 2000. Plant regeneration through somatic embryogenesis from anther cultures of C x R cultivar of coffee. Journal of Plantation Crops 28 (1): 61–67.
Munoz, L.C. and J.P. Baudoin, 1994. Influence of the cold pretreatment and the carbon source on callus induction from anthers in Phaseolus. Annu.Rep.Bean.Improv.Coop. 37: 129–130.
Munyon, I.P., J.F. Hubstenberger, and G.C. Phillips, 1989. Origin of plantlets and callus obtained from chile pepper anther cultures. In Vitro Cell Dev.Biol.J.Tissue Cult.Assoc. 25: 293–296.
Nagmani, R. and J.M. Bonga, 1985. Embryogenesis in subcultured callus of Larix decidua. Can.J.For.Res. 15: 1088–1091.
Nair, S., P.K. Gupta, and A.F. Mascarenhas, 1983. Haploid plants from in vitro anther culture of Annona squamosa Linn. Plant Cell Rep. 2: 198–200.
Narasimham, M. and P.B.K. Kishor, 1999. Induction of androgenesis in pigeonpea-possible reason for low response of pollen to tissue culture conditions. In: Plant Tissue Culture and Biotechnology: Emerging Trends. Univerities Press Ltd., Hyderabad, India pp. 232–239.
Nenova, N., M. Cristov, and P. Ivanov, 2000. Anther culture regeneration from some wild Helianthus species. Helia 23 (32): 65–72.
Nenova, N., P. Ivanov, and M. Christov, 1992. Anther culture regeneration of F1 hybrids of Helianthus annuus x H. smithii and H. annuus x H. eggerttii. In: Proceedings of the 13th International Sunflower Conference. Vol. 2. Pisa. pp.1509–1514.
Neuenschwander, B. and T.W. Baumann, 1995. Increased frequency of dividing microspores and improved maintenance of multicellular microspores of Goff - ea arabica in medium with coconut milk. Plant Cell Tiss.Org.Cult. 40 (1): 49–54.
Nishiyama, I. and S. Uematsu, 1967. Radiobiological studies in plants-XII. Embryogenesis following X-irradiation of pollen in Lycopersicon pimpinellifolium. Radiation Bot. 7: 481–489.
Nitsch, C., S. Andersen, M. Godard, M.G. Neuffer and W.F. Sheridan, 1982. Production of haploid plants of Zea mays and Pennisetum through androgenesis. In: Variability in Plants Regenerated from Tissue Culture. Earle, E.D. and Y. Demarly (Eds.), Praeger Publishers, New York, pp. 6991
Nitsch, C. and B. Norreel, 1973a. Effect of a thermic shock on the embryogenic power of the pollen of Datura innoxia cultivated in the anther or isolated from the anther. Acad.Sci Paris C.R.Ser.D. 276: 303–306.
Nitsch, C. and B. Norreel, 1973b. Factors favoring the formation of androgenetic embryos in anther culture. In: Genes Enzymes and Populations. International Latin American Symposium, Cali. pp. 129–144.
Nurhidayah, T., R. Horn, T. Röcher, and W. Friedt, 1996. High regeneration rates in anther culture of interspecific sunflower hybrids. Plant Cell Rep. 16: 167–173.
Ormerod, A.J. and P.D.S. Caligari, 1994. Anther and microspore culture of Lupinus albus in liquid culture medium. Plant Cell Tiss.Org.Cult. 36: 227–236.
Owen, H.R. and A.R. Miller, 1996. Haploid plant regeneration from anther cultures of three North American cultivars of strawberry (Fragaria x ananassa Duch.). Plant Cell Rep. 15: 905–909.
Owen, H.R., R.E. Veilleux, F.L. Haynes, and K.G. Haynes, 1988. Photoperiod effects on 2n pollen production, response to anther culture, and net photosynthesis of a diplandrous clone of Solanum phureja. Am.Potato.J. 65: 131–139.
Padmanabhan, C., M. Gurunathan, G. Pathmanabhan, and G. Oblisami, 1977. Induction of haploid plants from anther culture in Datura ferox L. Madras Agric.J. 64: 542–543.
Pal, A., 1983. Isolated microspore culture of the winged bean, Psophocarpus tetragonolobus (L) DC-growth, development and chromosomal status. Indian J.Exp.Biol. 21: 597–603.
Pallares, P., 1984. First results from ’in vitro’ culture of unfertilized cotton ovules (Gossypium hirsutum L.). Cotton et Fibres Tropicales 39 (4): 145–152.
Pandey, K.K., L. Przywara, and P.M. Sanders, 1990. Induced parthenogenesis in kiwifruit (Actinidia deliciosa) through the use of lethally irradiated pollen. Euphytica 51: 1–9
Pasakinskiene, I., K. Anamthawat-Jonsson, M.W. Humphreys, and R.N. Jones, 1997. Novel diploids following chromosome elimination and somatic recombination in Lolium multiflorum x Festuca arundinaceae hybrids. Heredity 78: 464–469.
Paulikova, D. and J. Vagera, 1993. In vitro induced androgenesis in Melandrium album. Biol.Plant. 35(4): 645–647.
Paz, M.M. and R.E. Veilleux, 1999. Influence of culture medium and in vitro conditions on shoot regeneration in Solanum phureja monoploids and fertility of regenerated doubled monoploids. Plant Breed. 118 (1): 53–57.
Pedroso, M.C. and M.S. Pais, 1997. Anther and microspore culture in Cammellia japonica. In: In Vitro Haploid Production in Higher Plants. Vol. 5. Jain, S.M., S.K. Sopory and R.E. Veilleux (Eds.) Kluwer Academic Publishers, Dordrecht, pp. 89–107
Pedroso, M.C. and S. Pais, 1993. Regeneration from anthers of adult Camellia japonica L. In Vitro Cell Dev.Biol.-Plant. 29P(4): 155–159.
Pehu, E., R.E. Veilleux, and K.W. Hilu, 1987. Cluster analysis of anther-derived plants of Solanum phureja (Solanaceae) based on morphological characteristics. Am.J.Bot. 74: 47–52.
Perez-Bermudez, P., M.J. Cornejo, and J. Segura, 1985. Pollen plant formation from anther cultures of Digitalis obscura. Plant Cell Tiss.Org.Cult. 5: 63–68.
Perri, E., M.V. Parlati, R. Mule, and A.S. Fodale, 1994. Attempts to generate haploid plants from in vitro cultures of Olea europaea L. anthers. Acta Hort. 356: 47–50.
Peters, J.E., O.J. Crocomo, W.R. Sharp, E.F. Paddock, I. Tegenkamp, and T. Tegenkamp, 1977. Haploid callus cells from anthers of Phaseolus vulgaris. Phytomorphol. 27: 79–85.
Piccirilli, M. and S. Arcioni, 1991. Haploid plants regenerated via anther culture in wild barley (Hordeum spontaneum C. Kock). Plant Cell Rep. 10 (67): 237–276.
Piper, J.G., B. Charlesworth, and D. Charlesworth, 1986. Breeding system evolution in Primula vulgaris and the role of reproductive assurance. Heredity 56: 207–217.
Pol’kheim, F., 1972. On the problem of selecting for breeding mutation chimeras and mutants in haploids of Pelargonium zonale Kleiner Liebling and Thuja gigantea gracilis. In: Eksperimental’nyi Mutagenez v Selektsii pp. 199–221.
Ponitka, A. and A. Slusarkiewicz-Jarzina, 1987. Induction of gynogenesis in selected plant species from the family Papilionaceae. Genetica Polonica 28 (3): 239–242.
Prakash, D.V.S.S.R., S. Chand, and P.B.K. Kishor, 1999. In vitro response from cultured anthers of Boswellia serrata Roxb. In: Plant Tissue Culture and Biotechnology: Emerging Trends. Universities Press Ltd., Hyderabad, India, pp. 226–231.
Prakash, J. and K.L. Giles, 1986. Production of doubled haploids in oriental lilie. In: Genetic Manipulation in Plant Breeding. Horn, W., C.J. Jensen, W. Oldenbach and O. Schieder (Eds.) Walter de Gruyter and Co, Berlin, pp. 335–337.
Prasad, B.R., M.A. Khadeer, P. Seeta, and S.Y. Anwar, 1991. In vitro induction of androgenic haploids in safflower (Carthamus tinctorius L.). Plant Cell Rep. 10(1):48–51.
Preil, W., W. Huhnke, M. Engelhardt, and M. Hoffmann, 1977. Haploids in Gerbera jamesonii from in vitro cultured capitulum explants. Z.Pflanzenzüchtg. 79: 167–171.
Qin, X. and G.L. Rotino, 1995a. Anther culture of several sweet and hot pepper genotypes. Acta Hort. 402: 313–316.
Qin, X. and G.L. Rotino, 1995b. Chloroplast number in guard cells as ploidy indicator of in vitro-grown androgenic pepper plantlets. Plant Cell Tiss.Org.Cult. 41 (2): 145–149.
Qu, Y., M.C. Mok, D.W.S. Mok, and J.R. Stang, 1988. Phenotypic and cytological variation among plants derived from anther cultures of Lilium longiflorum. In Vitro Cell Dev.Biol.J.Tissue Cult.Assoc. 24: 471–476.
Quarta, R., D. Nati, and F.M. Paoloni, 1992. Strawberry anther culture. Acta Hort. 300: 335–339.
Radijevic, L., N. Marinkovic, and S. Jevremovic, 2000. Influence of the sex of flowers on androgenesis in Aesculus hippocastanum L. anther culture. In Vitro Cell Dev.Biol.-Plant. 36 (6): 464–469.
Radojevic, L., L. Vapa, K. Borojevic, and J. Joksimovic, 1985. Plant regeneration in Saintpaulia ionantha Wendl. anther cultures. Savrem.Poljoprivreda 33: 485–491.
Raghavan, V. and R. Nagmani, 1989. Cytokinin effects on pollen embryogenesis in cultured anthers of Hyoscyamus niger. Can.J.Bot. 67: 247–257.
Raghuramulu, Y. and N.S. Prakash, 1996. Haploidy in coffee. In: In Vitro Haploid Production in Higher Plants. Vol. 3. Jain, S.M., S.K. Sopory and R.E. Veilleux (Eds.) Kluwer Academic Publishers, Dordrecht, pp. 349–363.
Rajasekaran, K. and M.G. Mullins, 1985. Somatic embryo formation by cultured ovules of Cabernet Sauvignon grape: effects of fertilization and of the male gameticide toluidine blue. Vitis 24: 151–157.
Ramulu, K., 1982. Genetic instability at the S-locus of Lycopersicon peruvianum plants regenerated from in vitro culture of anthers: generation of new S-specificities and S-allele reversions. Heredity 49 (3): 319–330.
Ranaweera, K.K.D.S. and R. Pathirana, 1992. Optimization of media and conditions for callus induction from anthers of sesame cultivar MI 3. J.Natn.Sci.Coun.Sri Lanka, 20 (2): 309–316.
Rao, I.U., I.V. Ramanuja, and M. Narasimham, 1986. Induction of androgenesis in the in vitro grown anthers of winged bean (Psophocarpus tetragonolobus). Phytomorphol. 36: 111–116.
Rao, P.V.L. and D.N. De, 1987. Haploid plants from in vitro anther culture of the leguminous tree, Peltophorum pterocarpum (DC) K. Hayne (Copper pod). Plant Cell Tiss.Org.Cult. 11(3): 167177.
Raquin, C., 1985. Induction of haploid plants by in vitro culture of Petunia ovaries pollinated with irradiated pollen. Z.Pflanzenzüchtg. 94: 166–169.
Raquin, C., A. Cornu, E. Farcy, D. Maizonnier, G. Pelletier, and F. Vedel, 1989. Nucleus substitution between Petunia species using gamma ray-induced androgenesis. Theor.Appl.Genet. 78: 337–341.
Ray, I.M. and E.T. Bingham, 1989. Breeding diploid alfalfa for regeneration from tissue culture. Crop Sci. 29: 1545–1548.
Regner, F., 1996. Anther and microspore culture in Capsicum. In: In Vitro Haploid Production in Higher Plants. Vol. 3. Jain, S.M., S.K. Sopory and R.E. Veilleux (Eds.) Kluwer Academic Publishers, Dordrecht, pp. 77–89.
Reynolds, T.L., 1985. Ultrastructure of anomalous pollen development in embryogenic anther cultures of Hyoscyamus niger. Am.J.Bot. 72: 44–51.
Reynolds, T.L., 1990. Interactions between calcium and auxin during pollen androgenesis in anther cultures of Solanum carolinense L. Plant Sci. 72 (1): 109–114.
Rivard, S.R., M.K. Saba-El-Leil, B.S. Landry, and M. Cappadocia, 1994. RFLP analyses and segregation of molecular markers in plants produced by in vitro anther culture, selfing, and reciprocal crosses of two lines of self-incompatible Solanum chacoense. Genome 37: 775–783.
Robert, T., A. San, and J. Pernes, 1989. Haploid selections in pearl millet (Pennisetum typhoides (Bunn.) Stapf et Hubb.): temperature effect. Genome 32: 946–952.
Rokka, V.M., C.A. Ishimaru, N.L.V. Lapitan, and E. Pehu, 1998. Production of androgenic dihaploid lines of the disomic tetraploid potato species Solanum acaule spp. acaule. Plant Cell Rep. 18 (12): 89–93.
Rose, J.B., J.M. Dunwell, and N. Sunderland, 1986a. Anther culture of Sorghum bicolor (L.) Moench. I. Effect of panicle pretreatment, anther incubation temperature and 2,4-D concentration. Plant Cell Tiss.Org.Cult. 6: 15–22.
Rose, J.B., J.M. Dunwell, and N. Sunderland, 1986b. Anther culture of Sorghum bicolor (L.) Moench. II. Pollen development in vivo and in vitro. Plant Cell Tiss.Org.Cult. 6: 23–31.
Rose, J.B., J.M. Dunwell, and N. Sunderland, 1987a. Anther culture of Lolium temulentum, Festuca pratensis and Lolium x Festuca hybrids. I. Influence of pretreatment, culture medium and culture incubation conditions on callus production and differentiation. Ann.Bot. 60: 191–201.
Rose, J.B., J.M. Dunwell, and N. Sunderland, 1987b. Anther culture of Lolium temulentum, Festuca pratensis and Lolium x Festuca hybrids. II. Anther and pollen development in vivo and in vitro. Ann.Bot. 60: 203–214.
Rose, J.B., R.P. Jones, and D.W. Simpson, 1993. Anther culture and intergeneric hybridization of Fragaria x ananassa. Adv.Strawb.Res. 12: 59–64.
Rotino, G.L., 1996. Haploidy in eggplant. In: In Vitro Haploid Production in Higher Plants. Vol. 3. Jain, S.M., S.K. Sopory and R.E. Veilleux (Eds.) Kluwer Academic Publishers, Dordrecht, pp. 115–141.
Rowe, P.R., 1974. Methods of producing haploids: parthenogenesis following interspecific hybridization. In: Haploids in Higher Plants. Advances and Potential. Kasha, K.J. (Ed.) University of Guelph, Guelph. pp. 43–52.
Rudramuniyappa, C.K., 1985. A histochemical study of developing sporogenous tissue and periplasmodial tapetum in the anther of Parthenium (Compositae). Cytologia 50: 891–898.
Rudramuniyappa, C.K. and B.G. Annigeri, 1985. Histochemical observations on the sporogenous tissue and tapetum in the anther of Euphorbia. Cytologia 50: 39–48.
Sacristan, M.D., 1971. Karyotypic changes in callus cultures from haploid and diploid plants of Crepis capillaris (L.) Waller. Chromosoma 33: 273–293.
Sairam, R.V. and N. Seetharama, 1996. Androgenetic response of cultured anthers and microspores of sorghum. Int.Sorghum Millets Newsl. 37: 69–71.
Saji, K.V. and M. Sujatha, 1998. Embryogenesis and plant regeneration in anther culture of sunflower (Helianthus annuus L.). Euphytica 103: 1–7.
Salunkhe, C.K., P.S. Rao, and M. Mhatre, 1999. Plant regeneration via somatic embryogenesis in anther callus of Vitis latifolia L. Plant Cell Rep. 18 (7/8): 670–673.
Samsudeen, K., K.N. Babu, M. Divakaran, and P.N. Ravindran, 2000. Plant regeneration from anther derived callus cultures of ginger (Zingiber officinale Rosc.). J.Hortic.Sci.Biotech. 75(4): 447450.
Sanguineti, M.C., R. Tuberosa, and S. Conti, 1990. Field evaluation of androgenetic lines of eggplant. Acta Hortic. 177–181.
Sangwan, R.S. and H. Camefort, 1984. Cold-treatment related structural modifications in the embryogenic anthers of Datura. Cytologia 49: 473–487.
Sankina, A.S. and L.S. Sankin, 1988. Characteristics of meiosis in the remote currant hybrid Ribes nigrum x Ribes “Holland Red” at the amphihaploid and the amphidiploid levels. Cytol.Genet. 22: 12–16.
Sarvesh, A., T.P. Reddy, and P.B. Kavi Kishor, 1993. Embryogenesis and organogenesis in cultured anthers of an oil yielding crop niger (Guizotia abyssinica Cass). Plant Cell Tiss.Org.Cult. 35: 75–80.
Sato, S., N. Katoh, H. Yoshida, S. Iwai, and M. Hagimori, 2000. Production of doubled haploid plants of carnation (Dianthus caryophyllus L.) by pseudofertilized ovule culture. Sci Hortic. 83 (3/4): 301–310.
Sator, C., G. Mix, and U. Menge, 1982. Investigations on anther culture of Lupinus polyphyllus. Landbauforsch.Volkenrode 32: 37–42.
Sauton, A. and R. Dumas-du-Vaulx, 1987. Induction of gynogenetic haploid plants in muskmelon (Cucumis melo L.) by use of irradiated pollen. Agron.Sci.Prod.Veg.Environ. 7: 141–147.
Savin, F., V. Decomble, M. Le-Couviour, and J. Hallard, 1988. The X-ray detection of haploid embryos arisen in muskmelon (Cucumis melo L.) seeds, and resulting from a parthenogenetic development induced by irradiated pollen. Rep.Cucurbit.Genet.Coop. pp. 39–42.
Schertz, K.F., 1963. Chromosomal, morphological, and fertility characteristics of haploids and their derivatives in Sorghum vulgare Pers. Crop Sci. 3: 445–447.
Schieder, O., 1976. Isolation of mutants with altered pigments after irradiating haploid protoplasts from Datura innoxia Mill. with X-rays. Mol.Gen.Genet. 149: 251–254.
Seran, T.H., K. Hirimburegama, W.K. Hirimburegama, and V. Shanmugarajah, 1999. Callus formation in anther culture of tea clones, Camellia sinensis (L.) O. Kuntze. J.Nat.Sci.Fund.Sri Lanka, 27 (3): 165–175.
Sharma, R. and S. Babber, 1990. In vitro studies of anther culture of Antirrhinum majus. Annals Biol.Ludhiana, 6(2): 175–178.
Shtereva, L.A., N.A. Zagorska, B.D. Dimitrov, M.M. Kruleva, and H.K. Oanh, 1998. Induced androgenesis in tomato (Lycopersicon esculentum Mill). II. Factors affecting induction of androgenesis. Plant Cell Rep. 18 (312): 317.
Sibi, M., R. Dumas-de-Vaulx, and D. Chambonnet, 1979. Obtainment of haploid plants through in vitro androgenesis in sweetpepper (Capsicum annuum L.). Ann.Amelior.Plant. 29: 583–606.
Simpson, E. and J.W. Snape, 1980. Haploid production in Hordeum spontaneum x Hordeum bulbosum crosses barley. Barley Genet.Newsl. 10: 66–67.
Singh, I.S. and A. Cornu, 1976. Research on androgenetic Petunia haploids with gynogenetic cytoplasmic pollen sterility. Ann.Amelior.Plant. 26: 565–568.
Singh, M.N. and R.N. Konar, 1979. In vitro induction of haploid roots and shoots from female gametophyte of Ephedre foliata Boiss. Beitr.Biol.Pflanzen. 55: 169–177.
Sinha, R.R. and K. Das, 1986. Anther-derived callus of Dolichos biflorus L., its protoplast culture and their morphogenic potential. Curr.Sci. 55 (9): 447–452.
Sinha, S., R.P. Roy, and K.K. Jha, 1979. Callus formation and shoot bud differentiation in anther culture of Solanum surattense. Can.J.Bot. 57 (22): 2524–2527.
Sink, K.C. and J.F. Reynolds, 1986. Tomato (Lycopesicon esculentum L.). In: Biotechnology in Agriculture and Forestry 2. Crops I. Bajaj, Y.P.S. (Ed.) Springer-Verlag, Berlin, pp. 319–344.
Sitbon, M., 1981. Production of haploid Gerbera jamesonii plants by in vitro culture of unfertilized ovules. Agron.Sci.Prod.Veg.Environ. 1: 807–812.
Skinner, D.Z. and G.H. Liang, 1996. Haploidy in alfalfa. In: In Vitro Haploid Production in Higher Plants. Vol. 3. Jain, S.M., S.K. Sopory and R.E. Veilleux (Eds.) Kluwer Academic Publishers, Dordrecht, pp. 365–375.
Slusarkiewicz-Jarzina, A. and M. Zenkteler, 1979. Cytological and embryological studies on haploids (n=3) of Crepis capillaris L. Bull. Soc.Amis.Sci.Lett.Poznan.Ser.D.Sti.Bio1. 65–73.
Smagula, J.M. and P.M. Lyrene, 1984. Blueberry. Handb.Plant Cell Cult. 3: 383–401.
Sniezko, R. and T. Visser, 1987. Embryo development and fruit-set in pear induced by untreated and irradiated pollen. Euphytica 36: 287–294.
Sommer, H.E. and H.Y. Wetzstein, 1984. Hardwoods. Handb.Plant Cell Cult. 3: 511–540.
Songstad, D.D. and B.V. Conger, 1988. Factors influencing somatic embryo induction from orchardgrass anther cultures. Crop Sci. 28: 1006–1009.
Starrantino, A. and P. Caponnetto, 1990. Effect of cytokinins on embryogenic callus formation from undeveloped ovules of orange. Acta Hort. 280: 191–194.
Stelly, D.M., J.A. Lee, and W.L. Rooney, 1988. Proposed schemes for mass-extraction of doubled haploids of cotton. Crop Sci. 28: 885–890.
Stoehr, M. and L. Zsuffa, 1990. Genetic evaluation of haploid clonal lines of a single donor plant of Populus maximowiczii. Theor.Appl.Genet. 80 (4): 470–474.
Strauss, A., F. Bucher, and P. J. King, 1981. Isolation of biochemical mutants using haploid mesophyll protoplasts of Hyoscyamus muticus. Planta 153: 75–80.
Suh, S.K. and H.G. Park, 1986. Studies on the anther culture of garlic (Allium sativum L.). I. Callus formation and plant regeneration. J.Korean Soc.Hortic.Sci. 27: 89–95.
Summers, W.L., 1997. Haploid plantlet production in tomato. In: In Vitro Haploid Production in Higher Plants. Vol. 5. Jain, S.M., S.K. Sopory and R.E. Veilleux (Eds.) Kluwer Academic Publishers, Dordrecht, pp. 219–231.
Svensson, M. and L.B. Johansson, 1995. Anther culture of Fragaria x ananassa: environmental factors and medium components affecting microspore divisions and callus production. J.Hortic.Sci. 69 (3): 473–480.
Takahata, Y., H. Komatsu, and N. Kaizyma, 1996. Microspore culture of radish (Raphanus sativus L.): influence of genotype and culture conditions on embryogenesis. Plant Cell Rep. 16: 163–166.
Tan, B.H. and G.M. Halloran, 1982. Pollen dimorphism and the frequency of inductive anthers in anther culture of Triticum monococcum. Biochem.Physiol.Pflanz. 177 (2): 197–202.
Tang, K., X. Sun, Y. He, and Z. Zhang, 1998. Anther culture response of wild Oryza species. Plant Breed. 117: 443–446.
Tanner, G.J., A.E. Moore, and P.J. Larkin, 1988. Reducing the ploidy of lucerne by anther culture or induced parthenogenesis. In: Ninth Australian Plant Breeding Conference, pp. 136.
Tanner, G.J., M. Piccirilli, A.E. Moore, P.J. Larkin, and S. Arcioni, 1990. Initiation of nonphysiological division and manipulation of developmental pathway in cultured microspores of Medicago sp. Protoplasma. 158 (3): 165–175.
Tefera, H., F.J. Zapata-Arias, R. Afza, and A. Kodym, 1999. Response of tef genotypes to anther culture. AgriTopia. 14 (1): 8–9.
Teixeira, J.B., M.R. Sondahl, and E.G. Kirby, 1994. Somatic embryogenesis from immature inflorescences of oil palm. Plant Cell Rep. 13: 247–250.
Teten Snider, K. and R.E. Veilleux, 1994. Factors affecting variability in anther culture and in conversion of androgenic embryos of Solanum phureja. Plant Cell Tiss.Org.Cult. 36: 345–354.
Thanh-Tuyen, N.T. and E.V. de Guzman, 1983a. Formation of pollen embryos in cultured anthers of coconut (Cocos nucifera L.). Plant Sci.Lett. 29 (1): 81–88.
Thanh-Tuyen, N.T. and E.V. de Guzman, 1983b. Pollen development stages for coconut anther culture. Kalikasan, Phylippine J.Biol. 12 (12): 135–144.
Theiler-Hedtrich, R. and C.S. Hunter, 1995. Regeneration of dihaploid chicory (Cichorium intybus L. var.foliosum Hegi) via microspore culture. Plant Breed. 114 (1): 18–23.
Thengane, S.R., M.S. Joshi, S.S. Khuspe, and A.F. Mascarenhas, 1994. Anther culture in Helianthus annuus L., influence of genotype and culture conditions on embryo induction and plant regeneration. Plant Cell Rep. 13: 222–226.
Thomas, T.D., A.K. Bhatanagar, M.K. Razdan, and S.S. Bhojwani, 1999. A reproducible protocol for the rediction of gynogenic haploids of mulberry, Morus alba L. Euphytica 110 (3): 169–173.
Todorova, M. and P. Ivanov, 1999. Induced parthenogenesis in sunflower: effect of pollen donor. Helia 22 (31): 49–56.
Todorova, M., P. Ivanov, P. Shindrova, M. Christov, and I. Ivanova, 1997. Doubled haploid production of sunflower (Helianthus annuus L.) through irradiated pollen-induced parthenogenesis. Euphytica. 97: 249–254.
Todorovic, R.R., P.D. Misic, D.M Petrovic, and M.A. Mirkowic, 1992. Anther culture of peach cultivars ‘Cresthaven’ and ’Vesna’. Acta Hort. 300: 331–333.
Tomasi, P., D.A. Dierig, R.A. Backhaus, and K.B. Pigg, 1999. Floral bud and mean petal length as morphological predictors of microspore cytological stage in lesquerella. HortSci. 34 (7): 1269–1270.
Tomes, D.T. and R.L. Peterson, 1981. Isolation of a dwarf plant responsive to exogenous GA3 from anther cultures of birdsfoot trefoil. Can.J.Bot. 59 (7): 1338–1342.
Torregrosa, L., 1998. A simple and efficient method to obtain stable embryogenic cultures from anthers of Vitis vinifera. Vitis. 37 (2): 91–92.
Tosca, A., L. Arcara, and P Frangi, 1999. Effect of genotype and season on gynogenesis efficiency in Gerbera. Plant Cell Tiss.Org.Cult. 59 (1): 77–80.
Tsay, H.S., J.Y. Hsu, T.P. Yang, and C.R. Yang, 1984. Anther culture of passion fruit (Passiflora edulis). J.Agric.Res.China. 33: 126–131.
Tsay, H. S., P. C. Lai, L. J. Chen, X. S. Cai, B. Z. Lai, and L. Z. Chen, 1982. Organ regeneration from anther callus of sweet potato. J.Agr.Res.China. 31 (2): 123–126.
Tsay, H. S. and C. Y. Su, 1985. Anther culture of papaya (Carica papaya L.). Plant Cell Rep. 4: 2830.
Tsay, H.S., C.C. Yeh, and J.Y. Hsu, 1990. Embryogenesis and plant regeneration from anther culture of bamboo (Sinocalamus latiflora (Munro) McClure). Plant Cell Rep. 9 (7): 349–351.
Tuberosa, R., M.C. Sanguineti, and S. Conti, 1987. Anther culture of egg-plant (Solanum melongena L.) lines and hybrids. Genetica Agraria. 41 (3): 267–274.
Turaev, A.M. and Z.B. Shamina, 1986. Optimization of the medium for cotton anther culture. Sov.Plant Physiol. 33: 439–444.
Turaev, A.M. and Z.B. Shamina, 1993. Cotton microspore culture in agarose. Sov.Plant Physiol. 40 (2): 276–279.
Tyagi, A.K., A. Rashid, and S.C. Maheshwari, 1981. Promotive effect of polyvinylpolypyrrolidone on pollen embryogenesis in Datura innoxia. Physiol.Plant. 53 (4): 495–406.
Tyukavin, G. B., N. A. Shmykova, and M. A. Monakhova, 1999. Cytological study of embryogenesis in cultured carrot anthers. J.Plant Physiol.(Russian). 46 (6): 767–773.
Uddin, M.R., M.M.J. Meyer, and J.J. Jokela, 1988. Plantlet production from anthers of eastern cottonwood (Populus deltoides). Can.J.For.Res. 18: 937–941.
Van-Eck, J.M. and S.L. Kitto, 1990. Callus initiation and regeneration in Mentha. HortSci. 25: 804–806.
van den Bulk, R.W. and J.M. van Tuyl, 1997. In vitro induction of haploid plants from the gametophytes of lily and tulip. In: In Vitro Haploid Production in Higher Plants. Vol.5. Jain, S.M., S.K. Sopory and R.E. Veilleux (Eds.) Kluwer Academic Publishers, Dordrecht, pp. 73–88.
Varghese, T.M. and G. Yadav, 1986. Production of embryoids and calli from isolated microspores of tomato (Lycopersicon esculentum Mill.) in liquid media. Biol.Plant. 28 (2): 126–129.
Vassileva-Dryanovska, O.A., 1966. The induction of haploid embryos and tetraploid endosperm nuclei with irradiated pollen in Lilium. Hereditas 55: 160–165.
Veilleux, R.E., J. Booze-Daniels, and E. Pehu, 1985. Anther culture of a 2n pollen producing clone of Solanum phureja Juz. and Buk. Can.J.Genet.Cytol. 27: 559–564.
Veuskens, J., D. Ye, M. Oliveira, D.D. Ciupercescu, P. Installe, H.A. Verhoeven, and I. Negrutiu, 1992. Sex determination in the dioecious Melandrium album androgenic embryogenesis requires the presence of the X chromosome. Genome 35: 8–16.
Vishukumar, U., M.S. Patil, and S.N. Nayak, 2000. Anther culture studies in pigeonpea. Karnataka J.Agri.Sci. 13 (1): 16–19.
Von-Aderkas, P. and J.M. Bonga, 1988. Formation of haploid embryoids of Larix decidua: early embryogenesis. Am.J.Bot. 75: 690–700.
Von-Aderkas, P., K. Klimaszewska, and J.M. Bonga, 1990. Diploid and haploid embryogenesis in Larix leptolepis, L. decidua, and their reciprocal hybrids. Can.J.For.Res. 20: 9–14.
Wakasa, K. and Y. Watanabe, 1979. Haploid plant of Oryza perennis (spontanea type) induced by anther culture. Japan.J.Breed. 29 (2): 146–150.
Wang, C.C., C.C. Chu, and C.S. Sun, 1975. The induction of Populus nigra pollen-plants. Acta Bot.Sinica 17: 56–59.
Wang, X.H., P.A. Lazzeri, and H. Lörz, 1993. Regeneration of haploid, dihaploid and diploid plants from anther-and embryo-derived cell suspensions of wild barley (Hordeum murinum). J.Plant Physiol. 141: 726–732.
Watanabe, K., 1977. Successful ovary culture and production of F1 hybrids and androgenic haploids in Japanese Chrysanthemum species. J.Hered. 68: 317–320.
Weatherhead, M.A., B.W.W. Grout, and K.C. Short, 1982. Increased haploid production in Saintpaulia ionantha by anther culture African violet. Sci.Hortic. 17: 137–144.
Wemicke, W., H. Lörz, and E. Thomas, 1979. Plant regeneration from leaf protoplasts of haploid Hyoscyamus muticus L. produced via anther culture. Plant Sci.Lett. 15: 239–249.
Wijowska, M., E. Kuta, and L. Przywara, 1999. In vitro culture of unfertilized ovules of Viola odorata L. Acta Biol.Cracoviensia 41: 95–101.
Willcox, M.C., S.M. Reed, J.A. Burns, and J.C. Wynne, 1990. Microsporogenesis in peanut (Arachis hypogaea). Aust.J.Bot. 77 (10): 1257–1259.
Wissemann, V, C. Mollers, and F.H. Hellwig, 1998. Microspore culture in the genus Rosa, further investigations. Angew.Botanik 72 (1–2): 7–9.
Wolff, D.W., R.E. Veilleux, and C.J. Jensen, 1986. Evaluation of anther-derived Streptocarpus x hybridus and their progeny. Plant Cell Tiss.Org.Cult. 6: 167–172.
Woo, S.-C., S.-W. Ko, C.-K. Wong, and X.-C. Wu, 1983. Anther culture of pollen plants derived from cross Oryza sativa L. x O. glaberrima Steud. Botanical Bull.Acad.Sinica 24 (1): 53–58.
Wu, H.N. and S.Z. Zhang, 1986. Effect of acridine yellow on development of anthers of Capsicum frutescens var. longum cultured in vitro. Chiang.su.Nung.Yeh.Hseuh.Pao.J.Agric.Sci. 2: 34–39.
Wu, J.Y., 1981. Obtaining haploid plantlets of crabapple from anther culture in vitro. Yuan.I.Hsueh.Pao.Acta Hortic.Sin. 8: 36.
Xue, G.R., K.W. Fei, and J. Hu, 1981. Induction of haploid plantlets of strawberry (Fragaria orientalis) by anther culture in vitro. Yuan.I.Hsueh.Pao.Acta Hortic.Sin. 8: 9–14.
Yadav, R.C., P.K. Sareen, and J.B. Chowdhury, 1988. High frequency induction of androgenesis in Ethiopian mustard (Brassica carinata A. Br.). Cruciferae Newsl. 13: 77.
Yang, Y.Q. and W.X. Wei, 1984. Induction of longan haploid plantlets from pollen cultured in certain proper media. Acta Genet.Sin. 11 (4): 288–293.
Zagorska, N. and B. Dimitrov, 1995. Induced androgenesis in alfalfa (Medicago saliva L.). Plant Cell Rep. 14: 249–252.
Zagorska, N., B. Dimitrov, P. Gadeva, and P. Robeva, 1997. Regeneration and characterization of plants obtained from anther cultures in Medicago sativa L. In Vitro Cell Dev.Biol.-Plant. 33 (2): 107–110.
Zagorska, N.A., M.D. Abadjieva, and H.K. Oanh, 1986. Factors affectng callus and plant production in anther cultures of tomato. In: Genetic Manipulation in Plant Breeding. Horn, W., C.J. Jensen, W. Oldenbach and O. Schieder (Eds.) Walter de Gruyter and Co., Berlin. pp. 361–363.
Zare, A.G., M. W. Humphreys, W.J. Rogers, and H.A. Collin, 1999. Androgenesis from a Lolium multiflorum x Festuca arundinacea hybrid to generate extreme variation for freezing-tolerance. Plant Breed. 118: 497–501.
Zheleznov, A.V., 1976. Methods of obtaining parthenogenetic haploids in buckwheat. In: Apomiksis i Ego Ispol’zovanie v Selektsii pp. 65–68.
Zhong, D., N. Michaux-Ferriére, and M. Coumans, 1995. Assay for doubled haploid (Helianthus annuus) plant production by androgenesis: fact or artifact? 1. In vitro anther culture. Plant Cell Tiss.Org.Cult. 41 (2): 91–97.
Zhou, C., 1989. Cell divisions in pollen protoplast culture of Hemerocallis fulva L. Plant Sci. 62 (2): 229–235.
Zhou, C., K. Orndorff, R.D. Allen, and A.E. DeMaggio, 1986. Direct observations on generative cells isolated from pollen grains of Haemanthus katherinae Baker. Plant Cell Rep. 5: 306–309.
Zhou, S.Q., D.Q. Qian, and X.Y. Cao, 1989. Haploid breeding and its cytogenetics in cotton (Gossypium hirsutum). In: Review of Advances in Plant Biotechnology, 1985–88. MujeebKazi, A. and L.A. Sitch (Eds.) CIMMYT, IRRI, Mexico, Manila, pp. 323–324.
Zhuang, X.J., C.Y. Hu, Y. Chen, and G.C. Yin, 1991. Embryoids from soybean anther culture. Soybean Genet.Newsl. 18: 265.
Zwierzykowski, Z., A.J. Lukaszewski, A. Lesniewska, and B. Naganowska, 1998. Genomic structure of androgenic progeny of pentaploid hybrids, Festuca arundinacea x Lolium multiflorum. Plant Breed. 117: 457–462.
Zwierzykowski, Z., E. Zwierzykowska, A. Slusarkiewicz-Jarzina, and A. Ponitka, 1999. Regeneration of anther-derived plants from pentaploid hybrids of Festuca arundinacea x Lolium multiflorum. Euphytica 105 (3): 191–195.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2003 Springer Science+Business Media New York
About this chapter
Cite this chapter
Maluszynski, M., Kasha, K.J., Szarejko, I. (2003). Published doubled haploid protocols in plant species. In: Maluszynski, M., Kasha, K.J., Forster, B.P., Szarejko, I. (eds) Doubled Haploid Production in Crop Plants. Springer, Dordrecht. https://doi.org/10.1007/978-94-017-1293-4_46
Download citation
DOI: https://doi.org/10.1007/978-94-017-1293-4_46
Publisher Name: Springer, Dordrecht
Print ISBN: 978-90-481-6393-9
Online ISBN: 978-94-017-1293-4
eBook Packages: Springer Book Archive