Abstract
We have been using microspore culture of Brassica at the Institute of Horticulture and Crop Sciences, Agricultural University of Norway, since 1989, our focus has been on Brassica oleracea (cabbage), Brassica napus (swede) and Brassica rapa (chinese cabbage and summer turnip rapeseed). The protocol presented here is based on over 12 years of experience. In the very beginning we visited Centre for Plant Breeding and Research, Wageningen, The Netherlands, the Horticultural Research International, Wellesbourne, UK and University of Guelph, Canada to discuss an optimal protocol for microspore culture in Brassica and our protocol includes components from all three places.
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Selected references for microspore culture in Brassica
Gland, A., R. Lichter and H.G. Schweiger, 1988. Genetic and exogenous factors affecting embryogenesis in isolated microspore cultures of Brassica napus L. J.Plant Physiol. 132: 613–617.
Guo, Y.-D. and S. Pulli, 1996. High-frequency embryogenesis in Brassica campestris microspore culture. Plant Cell Tiss.Org.Cult. 46: 219–225.
Hansen, M., 2000. ABA treatment and desiccation of microspore-derived embryos of cabbage (Brassica oleracea ssp. capitata L.) improves plant development. J.Plant Physiol. 156: 164–167.
Hansen, M. and K. Svinnset, 1993. Microspore culture of swede (Brassica napus ssp. rapifera) and the effects of fresh and conditioned media. Plant Cell Rep. 12: 496–500.
Lichter, R., 1982. Induction of haploid plants from isolated pollen of Brassica napus. Z.Pflanzenphysiol. 105: 427–434.
Rudolf, K., B. Bohanec and M. Hansen, 1999. Microspore culture of white cabbage, Brassica oleracea var. capitata L.: genetic improvement of non-responsive cultivars and effect of genome doubling agents. Plant Breed. 118: 237–241.
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© 2003 Springer Science+Business Media New York
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Hansen, M. (2003). Protocol for microspore culture in Brassica. In: Maluszynski, M., Kasha, K.J., Forster, B.P., Szarejko, I. (eds) Doubled Haploid Production in Crop Plants. Springer, Dordrecht. https://doi.org/10.1007/978-94-017-1293-4_32
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DOI: https://doi.org/10.1007/978-94-017-1293-4_32
Publisher Name: Springer, Dordrecht
Print ISBN: 978-90-481-6393-9
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