Abstract
The following procedure for large-scale production of doubled haploids (DH) in wheat at Svalöf Weibull AB (SW) is based on protocols developed in Sven Bode Andersen’s laboratory in Copenhagen (Andersen et al., 1987; Tuvesson et al.,1989). Since then the tissue culture programmes were rationalised to a few steps in order to speed up DH production and make the procedure cost effective. The described DH method is based on the knowledge that the response of the parental lines to anther culture influences the response of progeny combination (Tuvesson et al.,1989; Zhou and Konzak, 1992). At the same time it allows the practical use of anther culture technique in current breeding programmes. The goal is to produce DH from as many breeding combinations as possible rather than reaching high values for a few selected crosses. With a simple screening procedure of parental lines non-responding types are avoided. Circa 4,000 green wheat haploids are regenerated per year in both the screening experiments and the production. The method has been used successfully in French and Swedish winter wheat breeding programmes.
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© 2003 Springer Science+Business Media New York
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Tuvesson, S., von Post, R., Ljungberg, A. (2003). Wheat anther culture. In: Maluszynski, M., Kasha, K.J., Forster, B.P., Szarejko, I. (eds) Doubled Haploid Production in Crop Plants. Springer, Dordrecht. https://doi.org/10.1007/978-94-017-1293-4_12
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DOI: https://doi.org/10.1007/978-94-017-1293-4_12
Publisher Name: Springer, Dordrecht
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