Skip to main content
SpringerLink
Log in

Probe-Based (Hybridization) Methods

  • Chapter
DNA Methods in Clinical Microbiology

  • 212 Accesses

Abstract

The conceptual basis of the probe is outlined on pages 19/20. Probes have a variety of uses, which include:

  • Detection/identification of pathogens

  • Detection of specific (e.g. toxin) genes

  • Localization of an (intracellular) target sequence (in situ hybridization, ISH)

  • Confirmation of correct sequence in the products from nucleic acid amplification procedures (e.g. Chapter 4)

  • Probe-based amplification (ligase chain reaction: Chapter 6)

  • Typing (Chapter 7)

  • Detection of single-base mutations, e.g. those conferring resistance to antibiotics (line probe assay: Chapter 8)

  • Real-time indication of progress in a nucleic-acid amplification procedure (Chapters 4, 5)

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Log in via an institution
Chapter
USD 29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 129.00
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 169.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book
USD 169.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  • Bergmann JS, Yuoh G, Fish G & Woods GL (1999) Clinical evaluation of the enhanced Gen-Probe amplified Mycobacterium tuberculosis direct test for rapid diagnosis of tuberculosis in prison inmates. Journal of Clinical Microbiology 37: 1419–1425.

    PubMed  CAS  Google Scholar 

  • Bolton LF, Kelley LC, Lee MD et al (1999) Detection of multidrug-resistant Salmonella enterica serotype typhimurium based on a gene which confers cross-resistance to florfenicol and chloramphenicol. Journal of Clinical Microbiology 37: 1348–1351.

    PubMed  CAS  Google Scholar 

  • Cage GD & Saubolle MA (1997) Infrequently isolated and unusual mycobacterial species as agents of human disease. Reviews in Medical Microbiology 8: 125–135.

    Article  Google Scholar 

  • Desmond EP (1997) The role of the laboratory in the prevention and control of multidrugresistant tuberculosis. Reviews in Medical Microbiology 8: 81–90.

    Article  Google Scholar 

  • Fontana C, Favaro M, Frezza D & Favalli C (1999) Isolation and characterization of a DNA probe for Staphylococcus aureus subspecies aureus biovar. FEMS Microbiology Letters 172: 47–52.

    Article  PubMed  CAS  Google Scholar 

  • Heiter BJ & Bourbeau PP (1993) Comparison of the Gen-Probe Group A Streptococcus Direct Test with culture and rapid streptococcal antigen detection assay for diagnosis of streptococcal pharyngitis. Journal of Clinical Microbiology 31: 2070–2073.

    PubMed  CAS  Google Scholar 

  • Iwen PC, Walker RA, Warren KL et al (1995) Evaluation of nucleic acid-based test (PACE 2C) for simultaneous detection of Chlamydia trachomatis and Neisseria gonorrhoeae in endocervical specimens. Journal of Clinical Microbiology 33: 2587–2591.

    PubMed  CAS  Google Scholar 

  • Luk JM, Kongmuang U, Tsang RSW & Lindberg AA (1997) An enzyme-linked immunosorbent assay to detect PCR products of the rfbS gene from serogroup D salmonellae. Journal of Clinical Microbiology 35: 714–718.

    PubMed  CAS  Google Scholar 

  • Peterson EM, Darrow V, Blanding J et al (1997) Reproducibility problems with the AMPLICOR PCR Chlamydia trachomatis test. Journal of Clinical Microbiology 35: 957–959.

    PubMed  CAS  Google Scholar 

  • Ramael M, Segers K, Pannemans N et al (1995) Detection of human papillomavirus in cervical scrapings by in situ hybridization and the polymerase chain reaction in relation to cytology. Histochemical Journal 27: 54–59.

    Article  PubMed  CAS  Google Scholar 

  • Richter E, Niemann S, Rüsch-Gerdes S & Hoffner S (1999) Identification of Mycobacterium kansasii by using a DNA probe (AccuProbe) and molecular techniques. Journal of Clinical Microbiology 37: 964–970.

    PubMed  CAS  Google Scholar 

  • Spencer S & Macone A (1998) The appropriate use of molecular diagnostic assays for Chlamydia trachomatis testing. Gen-Probe Update 4: 1–3.

    CAS  Google Scholar 

  • Steed LL, Korgenski EK & Daly JA (1993) Rapid detection of Streptococcus pyogenes in pediatric patient specimens by DNA probe. Journal of Clinical Microbiology 31: 2996–3000.

    PubMed  CAS  Google Scholar 

  • Wollenhaupt J, Schnarr S & Kuipers JG (1998) Bacterial antigens in reactive arthritis and spondarthritis. Rational use of laboratory testing in diagnosis and follow-up. Baillière’s Clinical Rheumatology 12: 627–647.

    Article  CAS  Google Scholar 

Download references

Authors
  1. Paul Singleton
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

Copyright information

© 2000 Springer Science+Business Media Dordrecht

About this chapter

Cite this chapter

Singleton, P. (2000). Probe-Based (Hybridization) Methods. In: DNA Methods in Clinical Microbiology. Springer, Dordrecht. https://doi.org/10.1007/978-94-017-1286-6_3

Download citation

  • DOI: https://doi.org/10.1007/978-94-017-1286-6_3

  • Publisher Name: Springer, Dordrecht

  • Print ISBN: 978-90-481-5456-2

  • Online ISBN: 978-94-017-1286-6

  • eBook Packages: Springer Book Archive

Publish with us

Policies and ethics

Search

Navigation