Abstract
DNA polymorphism has became a widespread tool in biotechnology; in fact, they are frequently used for a number of technical approaches as, for example, in agronomic traits identification, variety characterisation, and marker-assisted breeding programmes. Coffea arabica is expected to show polymorphic DNA sequences as any other species and indeed some polymorphisms have been described. However, it has been reported that restriction fragment length polymorphism (RFLP) (Lashermes et al.,1996a) and polymorphism based on polymerase chain reactions (PCR, RAPD) (Orozco-Castillo et al., 1994; Lashermes et al., 1996b) have a relatively low degree of polymorphism (Paillard et al, 1993, 1996). To-date, a high degree of polymorphism has been found only through AFLP (Lashermes et al., 2000). As this species of coffee is autogamous and has a restricted genetic base, its heterozygosity is expected to be relatively low and the probability of finding a polymorphism is correspondingly reduced.
This is a preview of subscription content, log in via an institution.
Buying options
Tax calculation will be finalised at checkout
Purchases are for personal use only
Learn about institutional subscriptionsPreview
Unable to display preview. Download preview PDF.
References
Akkaya M.S., Bhagwat A.A., Cregan P.B. (1992), Length polymorphisms of simple sequence repeat DNA in soybean. Genetics, 132: 1131–1139
Bell C. J., Ecker, J.R. (1994), Assignment of 30 microsatellite loci to the linkage map of Arabidopsis. Genomics, 19: 137–144
Broun, P., Tanksley, S.D. (1996), Characterisation and genetic mapping of simple repeat sequences in the tomato genome. Mol. Gen. Genet, 250: 39–49
Don, R.H., Cox, P. T., Wainwright, B.J., Baker, K., Mattick, J.S. (1991), “Touchdown” PCR to circumvent spurious priming during gene amplification. Nucleic Acids Res, 19: 4008
Hecker, K.H., Roux, K.H. (1996), High and low annealing temperatures increase both specificity and yield in touchdown and stepdown PCR. Biotechniques, 20: 478–485
Kandpal, R.P., Kandpal, G., Weissman, S.M. (1994), Construction of libraries enriched for sequence repeats and jumping clones, and hybridisation selection for region-specific markers. Proc Nail Acad. Sci USA, 91: 88–92
Karagyozov, L., Kalcheva, I.D. Chapman, M. (1993), Construction of random small-insert genomic libraries highly enriched for simple sequence repeats. Nucleic Acids Res, 21: 3911–3912
Katzir, N., Danin-Poleg, Y., Tzuri, G., Karchi, Z., Lavi, U., Cregan, P.B. (1996), Length polymorphism and homologies of microsatellites in several Cucurbitaceae species. Theor Appl Genet, 93: 1282–1290
Lashermes, P., Cros, J., Combes, M.C., Trouslot, P., Anthony, F., Hamon S., Charrier A. (1996a), Inheritance and restriction fragment length polymorphism of chloroplast DNA in the genus Coffea L. Theor. Appl. Genet, 93: 626–632
Lashermes, P., Trouslot, P., Anthony, F., Combes, M.C., Charrier, A. (1996b), Genetic diversity for RAPD markers between cultivated and wild accessions of Coffea arabica. Euphytica, 87: 59–64
Lashermes, P., Andrzejewski, S., Bertrand, B., Combes, M.C., Dussert, S., Graziosi, G., Trouslot, P., Anthony, F. (2000), Molecular analysis of introgressive breeding in coffee (Coffea arabica L.). Theor Appl Genet, 100: 139–146
Levinson, G., Gutman, G.A. (1987), High frequencies of short frameshifts in poly-CA/TG tandem repeats borne by bacteriophage M13 in Escherichia coli K-12. Nucleic Acids Res, 15: 5323–5338
Ma, Z.Q., Roder, M., Sorrells, M. E. (1996), Frequencies and sequence characteristics of di-, tri-, and tetra-nucleotide microsatellites in wheat. Genome, 39: 123–130
Mellersh C., Sampson, J. (1993), Simplifying detection of microsatellite length polymorphisms. Biotechniques, 15: 582–584
Morgante M., Olivieri A.M. (1993), PCR-amplified microsatellites as markers in plant genetics. Plant J, 3: 175–182
Morgante, M., Rafalski, A., Biddle, P., Tingey, S., Olivieri, A.M. (1994), Genetic mapping and variability of seven soybean simple sequence repeat loci. Genome, 37: 763–769
Morgante, M., Pfeiffer, A., Jurman, I., Paglia, G., Olivieri, A.M. (1998), Microsatellite markers in plants. In-Molecular tools for screening biodiversity. Plants and animals. Karp, A., Isaac, P. G., Ingram, D.S. (eds.), Chapman and Hall, pp. 206–208 & 288–296
Murray, M.G., Thompson, W.F. (1980), Rapid isolation of high molecular weight plant DNA. Nucleic Acids Res, 8: 4321–4325
Orozco-Castillo, C., Chalmers, K.J., Waugh, R., Powell, W. (1994), Detection of genetic diversity and selective gene introgression in coffee using RAPD markers. Theor Appl Genet, 87: 934–940
Paillard, M., Duchateau, N., Petiard, V. (1993), Diversité génétique de quelques groupes de caféiers: utilisation des outils moléculaires, RFLP et RAPD. Paper presented at ASIC, 15e Colloque, Montpellier, pp. 33–40
Paillard, M., Lashermes, P., Petiard, V, (1996), Construction of a molecular linkage map in coffee. Theor App/ Genet, 93, 41–47
Panaud, O., Chen, X., McCouch, S. R. (1996), Development of microsatellite markers and characterisation of simple sequence length polymorphism (SSLP) in rice (Oryza sativa L.). Mol. Gen. Genet, 252: 597–607
Rafalski, J.A., Vogel, J.M., Morgante, M., Powell, W., Andre, C., Tingey, S.V. (1996), Generating and using DNA markers in plants. In- Nonmammalian genomic analysis: a practical guide, Birren, B., Lai, E. (eds), Academic Press, London New York, pp. 75–134.
Saghi Maroof, M. A., Biyashev, R.M., Yang, G. P., Zhang, Q., Allard, R.W. (1994), Extraordinarily polymorphic microsatellite DNA in barley: species diversity, chromosomal locations and population dynamics. Proc Nail Acad Sc, USA, 91: 5466–5470
Schlotterer, C., Tautz, D. (1992), Slippage synthesis of simple sequence DNA. Nucleic Acids Res, 20: 211–215
Tautz, D., Renz, M. (1984), Simple sequences are ubiquitous repetitive components of eucaryotic genomes. Nucleic Acids Res, 12: 4127–4138
Vascotto, F., Degli Ivanissevich, S., Rovelli, P., Anthony, F., Anzueto, F., Lashermes, P., Graziosi, G. (1999), Microsatellites in Coffea arabica: Construction and Selection of Two Genomic Libraries.Paper presented at III SIBAC, 24–28 May, Londrina, Brazil
Wang, Z., Weber, J.L., Zhong, G., Tanksley, S.D. (1994), Survey of plant short tandem DNA repeats. Theor App! Genet, 88: 1–6
Wu, K.S, Tanksley, S.D. (1993), Abundance, polymorphism and genetic mapping of microsatellites in rice. Mol. Gen. Genet, 241: 225–235
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2000 Springer Science+Business Media Dordrecht
About this chapter
Cite this chapter
Rovelli, P., Mettulio, R., Anthony, F., Anzueto, F., Lashermes, P., Graziosi, G. (2000). Microsatellites in Coffea Arabica L.. In: Sera, T., Soccol, C.R., Pandey, A., Roussos, S. (eds) Coffee Biotechnology and Quality. Springer, Dordrecht. https://doi.org/10.1007/978-94-017-1068-8_9
Download citation
DOI: https://doi.org/10.1007/978-94-017-1068-8_9
Publisher Name: Springer, Dordrecht
Print ISBN: 978-90-481-5565-1
Online ISBN: 978-94-017-1068-8
eBook Packages: Springer Book Archive