Abstract
This research was undertaken to study the mode of action of low O2 in delaying ripening of avocado fruit. Hass avocado fruits were treated with propylene in various O2 concentrations (1%, 2%, 5%, and 21%) at 20°C and ethylene production and ripening of the fruit were followed by measuring accumulation of ACC, EFE activity and the polygalacturonase (PG) and cellulase (Cx) activities respectively. Low O2 delayed the softening of propylene treated fruits and prevented the rise in EFE activity. Furthermore, low O2 suppressed the rise in PG and Cx activities. Gel electrophoresis and western blot analysis showed that the levels of PG and Cx proteins, were drastically reduced in fruits held in low O2 (1% and 2%).
This work was conducted to fulfil partly the requirements for M. S. and Ph. D. at Mediterreanean Agronomic Institute-Chania and Aristotle University-Thessaloniki respectively.
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References
Awad, M., and Young, R. E. (1979) Postharvest variation in cellulase, polygalacturonase and pectinmethylesterase in avocado (Persea americana Mill. cv Fuerte) fruits in relation to respiration and ethylene production’, Pl. Physiol. 64, 306–308.
Ben-Aire, R., Kislev, N. and Frenkel, C. (1979), ‘Ultrastructural changes in the cell walls of ripening apple and pear fruit’, Plant Physiol. 64, 197–202.
Bennet, A. B. and Christoffersen R. E. (1986), ‘Synthessis and processing of cellulase from ripening avocado fruit’ Plant Physio1.81. 830–835.
Boller, T., Herner R. C., and Kende H. (1979), ‘ Assay for and enzymatic formation of an ethylene precursor, 1-Aminocyclopropane-l-Carboxylic Acid’ Planta 145, 293–303.
Bradford,M. M. (1976),’A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dyebinding’Anal Biochem 72, 248–254.
Brady, C. J, (1987) ‘Fruit ripening’, Ann. Rev. Plant Physiol. 38, 155–178.
Burg, S. P. and Burg E. A. (1967) ‘Molecular requirements for biological activity of ethylene’, Plant Physiol. 42, 144–152.
Cameron, A. C. C., Fenton A. G., Y. Yu., Adams D., and Yang S.F. (1979), ‘Increased production of ethylene by plant tissues with ACC’ Hort Science 14, 178–180.
Christoffersen, R.E., Tucker M.L. and Laties G.G. (1984) ‘Cellulase gene expression in ripening avocado fruit the accumulation of cellulase mRNA and protein as demonstrated by cDNA hybridizationand immunodetection’, Plant Mol. Biol. 3, 385–392.
Guy, M. and H. Kende (1984) ‘Conversion of 1-aminocyclopropane-l-carboxyl acid to ethylene by isolated vacuoles of Pisum sativum L.’, Planta 160, 281–287.
Kader, A. A. (1986) ‘Biochemical and physiological basis for effects of controlled and modified atmospheres on fruits and vegetables’, Food Tecnology 40, 99–104.
Kanellis, A. K. (1987) ‘Stydies on the suppressive effect of 25% 02 on the rate of ripening, activitiess of hydrolytic enzymes and gene expression in ripening fruit Ph.D, Dissertation, University of Marylant.
Kanellis, A. K. Solomos T., Mattoo A. K. (1989a) ‘Hydrolytic enzyme activities and protein pattern of avocado fruit ripened in air and in low oxygen, with and without ethylene’, Plant Physiol. 90, 257–264.
Kanellis A. K., Solomos, T., Mehta, M.T., Mattoo. A.K. (1989b) ‘Decreased cellulase activity in avocado fruit subjected to 2.5% 02 correlates with lower cellulase protein and gene transcript levels’, Plant Cell Physiol 30, 817–823.
Kidd, F. and West, C. (1945) Respiratory activity and duration of life of apples gathered at different stages of development and subsequently maintained at a constant temperature’, Plant Physiol. 20, 467–504.
Lizada M.C.C. and Yang S. F. (1979) ‘A simple and Sensitive Assay for lAminocyclopropane-1-Carboxylic Acid’, Anal. Biochem. 100, 140–145.
Laemmii, U.K. (1970) ‘Cleavage of structural proteins during the assembly of the head of bacteriophage T, Nature 227, 680–685.
Nelson, N. (1944) ‘A Photometric adaptation of the Somogyi method for the determination of glucose’, J. Biol, Chem 153, 375–380.
Pesis, E., Y. Fuchs, and G. Zaubermn (1978) ‘Cellulose activity and fru it softening in avocado’, Plant. Physiol. 61, 416–419.
Pratt Aloia Ka, Thomson W. W, Young R. E (1980) ‘Ultrastractural changes in the walls of ripening avocados: transmition scaring and freeze fracture microscopy’, Bot Gaz 141, 366–373.
Sachs MM, Freeling M, Okimoto R. (1980) ‘The anaerobic proteins of maize’,Cell 20, 761–676.
Towbin, H., Staeheliin T., and Gordon J. (1979) ‘Electrophoresic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications’, Proc. Nat. Acad. Sci. USA 76, 4350–4354.
Tucker, M. L, Laties, G. G. (1984) ‘Interrelationship of gene expression, polysome prevalence and respiration during ripening of ethylene and/or cyanide treated avocado fruit, Persea americans cultivar Hass’, Plant Physiol. 74, 307–315.
Tucker, M. L., Durbin M. L., Clegg M. T. and Lewis N.L. (1987) ‘Avocado cellulase: nucleotide sequence of a putative full-length cDNA clone and evidence for a small gene familly’, Plant Mol. Biol. 9, 197–203.
Yang, S.F. and Hoffman N.E. (1984) ‘Ethylene biosynthesis and its regulation in hither plants’, Ann. Rev. Plant Physiol. 35, 155–189.
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© 1993 Springer Science+Business Media Dordrecht
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Metzidakis, J., Sfakiotakis, E. (1993). Inhibition of Ethylene Biosynthesis and Supression of Cellulase and Polygalacturonase in Avocado Fruit Subjected to Low Oxygen Storage. In: Pech, J.C., Latché, A., Balagué, C. (eds) Cellular and Molecular Aspects of the Plant Hormone Ethylene. Current Plant Science and Biotechnology in Agriculture, vol 16. Springer, Dordrecht. https://doi.org/10.1007/978-94-017-1003-9_26
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DOI: https://doi.org/10.1007/978-94-017-1003-9_26
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