Skip to main content
SpringerLink
Log in

Conifer Micropropagation : Applied Research and Commercial Aspects

  • Chapter
Cell and Tissue Culture in Forestry

Part of the book series: Forestry Sciences ((FOSC,volume 24-26))

Abstract

Ten years ago, the main method to produce plants of coniferous species for reforestation was by seedling production. Only Cryptomeria japonica, Thuya sp. and Cupressocyparis leylandii and some other ornamental species were propagated by rooted cuttings. However, evolution of vegetative multiplication techniques of woody plants has altered the situation. Today, numerous species of coniferous trees are propagated by rooted cuttings, among them: Picea abies, Picea glauca, Picea sitchensis, Larix sp., Sequoia sempervirens, Sequoiadendron giganteum etc. Often the starting material is selected juvenile seedlings, but in some cases (Sequoia sempervirens, Pinus radiata, etc.) selection and vegetative multiplication of adult trees is possible (we consider trees older than 7 or 8 years to be sufficiently adult for selection). Difficulties linked with vegetative propagation of adult genotypes are correlated with maturation of trees. This is especially true for coniferous species. Generally, cuttings taken from seedlings or young plants root easily and manifest a growth habit and vigor characteristic of plants derived from seeds. Nevertheless with some species, even at this very young stage, problems are encountered in vegetative propagation. Classical examples are Pseudotsuga menziesii, Abies sp., and Araucaria sp. When cuttings are taken from adult material and rooted, the vigor of these plants is poor and they are often plagiotropic (see chapter on rejuvenation). In some species with certain treatments (pruning for example) it is possible to regain eventually an orthotropic growth habit.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Log in via an institution
Chapter
USD 29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 169.00
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 219.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book
USD 219.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. ABOEL NIL M, ZS WOCKOK 1978 Morphogenetic heterogeneity of cultured Douglas fir cotyledons and its correlation with seed weight. Proc 4th Int Congr Plant Tissue Cell Cul. Univ Calgary, Alberta Canada

    Google Scholar 

  2. AITKEN-CHRISTIE J, TA THORPE 1984 Clonal propagation. gymnosperms in cell culture and somatic cell genetics of plants. In Cell Culture and Somatic Cell Genetics of Plants, Vol 1, Academic Press, pp 82–95

    Google Scholar 

  3. BALL E, DM MORRIS, JA RYDELIUS 1978 Cloning of Sequoia sempervirens from mature trees through tissue culture. In Proc Round Table Conf In vitro“ Multiplication Woody Species, Gembloux, Belgium, June 6–8, 1978, pp 181–226

    Google Scholar 

  4. BEKKAOUI F 1983 Microbouturage in vitro du Sequoia sempervirens (Endl). Stade de la rhizogenèse considéré comme critère de juvénilité chez deux clones d’âges différents. DEA Paris VI, 43 p

    Google Scholar 

  5. BEKKAOUI F, A FRANCLET, N WALKER 1985 Culture in vitro de méristèmes de douglas âgés et juvéniles. Ann AFOCEL 1984, pp 5–72

    Google Scholar 

  6. BORNMAN CH, E JANSSON 1980 Organogenesis in cultured Pinus sylvestris tissue. Z Pflanzenphysiol 96: 1–6

    CAS  Google Scholar 

  7. BOULAY M 1976 Recherches sur la propagation du douglas par culture in vitro. Ann AFOCEL 1975, pp 84–145

    Google Scholar 

  8. BOULAY M 1978 Multiplication rapide du Sequoia sempervirens en culture in vitro. Ann AFOCEL 1977, pp 38–65

    Google Scholar 

  9. BOULAY M 1985 Aspects pratiques de la multiplication in vitro des essences foretières. Ann AFOCEL 1984, pp 7–43

    Google Scholar 

  10. CHENG TY 1975 Adventitious bud formation in culture of douglas fir (Pseudotsuga menziesii Mirb.) Franco. Plant Sci Lett 5: 97103

    Google Scholar 

  11. CHOULET B, A ROBERT, T HIGUGHI, F BARNOUD 1964 Quelques aspects chimiques et biochimiques des ligno-celluloses synthétisées dans une souche de Sequoia sempervirens. Actes Symp Int Grenoble, pp 481–490

    Google Scholar 

  12. COLEMAN WK, TA THORPE 1977 In vitro culture of western red cedar (Thuya plicata Don) I–Plantlet formation. Bot Gaz 138: 298–304

    Article  CAS  Google Scholar 

  13. DAVID H, K ISEMUKALI, A DAVID 1978 Obtention de pin maritime (Pinus pinaster Sol.) à partir de brachyblastes ou d’apex caulinaires de très jeunes sujets cultivés in vitro. C R Aced Sci, Paris 287: 245–247

    Google Scholar 

  14. DAVID A, H DAVID, M FAYE, K ISEMUKALI 1979 Culture in vitro et micropropagation du pin maritime (Pinus pinaster Sol.). AFOCEL Etud Rech 12: 33–40

    Google Scholar 

  15. DAVID A, H DAVID, T MATEILLE 1982 In vitro adventitious budding on Pinus pinaster cotyledons and needles. Physiol Plant 56: 102–107

    Article  CAS  Google Scholar 

  16. DINER A, R MOTT 1982 A rapid axenic assay for hypersensitive resistance of Pinus lambertiana to Cronartium ribicola:. Phytopathology 72: 864–865

    Google Scholar 

  17. ENGELMANN F 1982 Multiplication végétative in vitro de clones juvéniles et matures de Cunninghamia lanceolata:. DEA Univ Pierre et Marie Curie, Paris VI, pp 43

    Google Scholar 

  18. FOURET Y 1984 Micropropagation in vitro du Sequoia sempervirens (Endl.): effet de la fréquence des repiquages sur le rajeunissement de trois clones issus de matériel d’âge différent et recherche d’un critère précoce de juvénilité. DEA Univ Pierre et Marie Curie, Paris VI, pp 43

    Google Scholar 

  19. FRANCLET A, A DAVID, H DAVID, M BOULAY 1980 Première mise en évidence morphologique d’un rajeunissement de méristèmes primaires caulinaires de pin maritime âgé (Pinus pinaster Sol.) C R Acad Sci, Paris 290: 927–930

    Google Scholar 

  20. HRIB J, V RYPACEK 1983 In vitro testing for the resistance of conifers to fungus Phaseolus schweinitzii (Fr.) Pat. on callus cultures. Eur J For Pathol 13: 86–91

    Google Scholar 

  21. KIM JH, SK HYUN, SK LEE, KJ LEE, KC KIM 1982 Mass clonal propagation of Pinus rigida x taeda Fl by embryo culture. Res Rep Inst For Genet, Off For, Suweon-Korea, V 18, pp 74–79

    Google Scholar 

  22. KOLEVSKA-PLETIKAPIC B, S JELASKA, J BERLJAK, M VIDAKOVIK 1983 Bud and shoot formation in juvenile tissue culture of Pinus nigra:. Silvae Genet 32: 115–119

    Google Scholar 

  23. La GOUBLAYE de NANTOIS T de 1980 Rajeunissement chez le douglas (Pseudotsuga menziesii) en vue de la propagation végétative. Etude sur la plagiotropie des parties aériennes et racinaires. DEA Univ Paris VI, pp 44

    Google Scholar 

  24. La GOUBLAYE de NANTOIS H de 1981 Vieillissement et rajeunissement chez le Sequoia sempervirens (Endl.) en relation avec la propagation végétative. Thèse 3ème Cycle Univ Paris VI, pp 170

    Google Scholar 

  25. LEACH GN 1979 Growth in soil of plantlets produced by tissue cultured loblolly pine. TAPPI 62: 59–61

    Google Scholar 

  26. LIBBY WJ 1983 The clonal option. Norsk Inst Skogforskning 1432, AS.NLH Norway, pp 3–32

    Google Scholar 

  27. McKEAND SE, HL ALLEN 1984 Nutritional and root development factors affecting growth of tissue cultured plantlets of loblolly pine. Physiol Plant 61: 523–528

    Article  CAS  Google Scholar 

  28. MEHRA-PALTA A, RH SMELTZER, R MOTT 1978 Hormonal control of induced organogenesis. Experiments with excised plant parts of loblolly pine. TAPPI 61: 37–40

    Google Scholar 

  29. MISSON JP, M COUMANS, P GIOT-WIRGOT, Th GASPAR 1982 Induction de bourgeons adventifs de Picea pungens en culture in vitro:. Z Pflanzenphysiol 107: 161–167

    Google Scholar 

  30. MONTEUUIS 0 1984 Etude de la multiplication végétative de Sequoiadendron giganteum ( Lindl.) en vue du clonage. DEA Clermont II, pp 30

    Google Scholar 

  31. MOTT RL, HV AMERSON 1981 A tissue culture process for the clonal production of loblolly pine plantlets. Tech Bull n° 271, North Carolina Agric Res Serv, pp 1–14

    Google Scholar 

  32. POISSONNIER M, A FRANCLET, MJ DUMANT, JY GAUTRY 1980 Enracinement de tigelles issues in vitro de Sequoia sempervirens. Ann AFOCEL 1979, pp 232–253

    Google Scholar 

  33. RANCILLAC M, M FAYE, A DAVID 1982 In vitro rooting of cloned shoots in Pinus pinaster:. Physiol Plant 56: 97–101

    Article  CAS  Google Scholar 

  34. REILLY K, CH BROWN 1976 In vitro studies of bud and shoot formation in Pinus radiata and Pseudotsuga menziesii:. Georgia For Res Pap 86, pp 1–9

    Google Scholar 

  35. SOMMER HE, CH BROWN, P KORMANIK 1975 Differentiation of plantlets in long leaf pine (Pinus palustris) Mill. tissue cultured in vitro. Bot Gaz 136: 196–200

    Article  Google Scholar 

  36. STINE M, H SOMMER 1983 Adventitious shoot formation on embryos of Pinus palustris Mill., Pinus elliottii Engelm. and Pinus palustris x elliottii:. In 17th Southern For Tree Improv Conf, pp 349–350

    Google Scholar 

  37. VON ARNOLD S, T ERIKSSON 1979 Induction of adventitious buds of Norway spruce (Picea abies) grown in vitro. Physiol Plant 45: 29–34

    Article  Google Scholar 

  38. WALKER N, E DUMAS, A FRANCLET, F BEKKAOUI 1985 Techniques de culture in vitro des méristèmes de Sequoia sempervirens et Pinus pinaster. Ann AFOCEL 1984: pp 87–109

    Google Scholar 

  39. YOUNG PM, AS HUTCHINS, ML CANFIELD 1984 Use of antibiotics to control bacteria in shoot cultures of woody plants. Plant Sci Lett 34: 203–209

    Article  CAS  Google Scholar 

  40. ZOBEL B 1981 Vegetative propagation in forest management operations.In Proc 16th Southern For Tree Improv Conf Blacksburg VA, pp 149–159

    Google Scholar 

Download references

Authors
  1. M. Boulay
    View author publications

    You can also search for this author in PubMed Google Scholar

Editor information

Editors and Affiliations

  1. Canadian Forestry Service, Canada

    J. M. Bonga

  2. Davis, Department of Pomology, University of California, 1035 Wickson Hall, 95616, Davis, Calif., USA

    Don J. Durzan

Rights and permissions

Reprints and permissions

Copyright information

© 1987 Springer Science+Business Media Dordrecht

About this chapter

Cite this chapter

Boulay, M. (1987). Conifer Micropropagation : Applied Research and Commercial Aspects. In: Bonga, J.M., Durzan, D.J. (eds) Cell and Tissue Culture in Forestry. Forestry Sciences, vol 24-26. Springer, Dordrecht. https://doi.org/10.1007/978-94-017-0992-7_15

Download citation

  • DOI: https://doi.org/10.1007/978-94-017-0992-7_15

  • Publisher Name: Springer, Dordrecht

  • Print ISBN: 978-90-481-8301-2

  • Online ISBN: 978-94-017-0992-7

  • eBook Packages: Springer Book Archive

Publish with us

Policies and ethics

Search

Navigation