A Novel Technology for Conversion of In Vivo Tissue Architecture into a Three-Dimensional In Vitro Culture Mode

Abstract

Tissue engineering has advanced remarkably to the point that three-dimensional multicellular organoids can be reconstructed by supplying appropriate scaffold(s) to dispersed cells in culture. However, organoid design reflective of hierarchical tissue architectures based on in vivo cell lineages is still difficult. We created a novel means to convert in vivo hierarchical tissue architectures into three-dimensional in vitro culture modes through our two newly established technologies. We devebped a method of organ engineering to remodel a rat liver into a reconstructed organoid, without separating the majority of liver cells, by a continuous three-step perfusion via the portal vein. Our other method is a breakthrough technology for culturing cells on TOSHI-substrata (substrata made of tissue/organ sections for histopathology) that conserve the microarchitecture and component of the original tissue in vivo.